Job ID = 12531714 SRX = SRX5811095 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 32842524 spots for SRR9033911/SRR9033911.sra Written 32842524 spots for SRR9033911/SRR9033911.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:31:30 32842524 reads; of these: 32842524 (100.00%) were paired; of these: 16795206 (51.14%) aligned concordantly 0 times 13015935 (39.63%) aligned concordantly exactly 1 time 3031383 (9.23%) aligned concordantly >1 times ---- 16795206 pairs aligned concordantly 0 times; of these: 1022525 (6.09%) aligned discordantly 1 time ---- 15772681 pairs aligned 0 times concordantly or discordantly; of these: 31545362 mates make up the pairs; of these: 30281305 (95.99%) aligned 0 times 333873 (1.06%) aligned exactly 1 time 930184 (2.95%) aligned >1 times 53.90% overall alignment rate Time searching: 00:31:30 Overall time: 00:31:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 4381685 / 17019817 = 0.2574 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 17 Apr 2021 10:07:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5811095/SRX5811095.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5811095/SRX5811095.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5811095/SRX5811095.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5811095/SRX5811095.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 17 Apr 2021 10:07:10: #1 read tag files... INFO @ Sat, 17 Apr 2021 10:07:10: #1 read treatment tags... INFO @ Sat, 17 Apr 2021 10:07:17: 1000000 INFO @ Sat, 17 Apr 2021 10:07:24: 2000000 INFO @ Sat, 17 Apr 2021 10:07:31: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 17 Apr 2021 10:07:38: 4000000 INFO @ Sat, 17 Apr 2021 10:07:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5811095/SRX5811095.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5811095/SRX5811095.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5811095/SRX5811095.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5811095/SRX5811095.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 17 Apr 2021 10:07:40: #1 read tag files... INFO @ Sat, 17 Apr 2021 10:07:40: #1 read treatment tags... INFO @ Sat, 17 Apr 2021 10:07:45: 5000000 INFO @ Sat, 17 Apr 2021 10:07:47: 1000000 INFO @ Sat, 17 Apr 2021 10:07:52: 6000000 INFO @ Sat, 17 Apr 2021 10:07:55: 2000000 INFO @ Sat, 17 Apr 2021 10:08:00: 7000000 INFO @ Sat, 17 Apr 2021 10:08:03: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 17 Apr 2021 10:08:07: 8000000 INFO @ Sat, 17 Apr 2021 10:08:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5811095/SRX5811095.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5811095/SRX5811095.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5811095/SRX5811095.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5811095/SRX5811095.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 17 Apr 2021 10:08:10: #1 read tag files... INFO @ Sat, 17 Apr 2021 10:08:10: #1 read treatment tags... INFO @ Sat, 17 Apr 2021 10:08:10: 4000000 INFO @ Sat, 17 Apr 2021 10:08:15: 9000000 INFO @ Sat, 17 Apr 2021 10:08:17: 1000000 INFO @ Sat, 17 Apr 2021 10:08:18: 5000000 INFO @ Sat, 17 Apr 2021 10:08:22: 10000000 INFO @ Sat, 17 Apr 2021 10:08:25: 2000000 INFO @ Sat, 17 Apr 2021 10:08:26: 6000000 INFO @ Sat, 17 Apr 2021 10:08:30: 11000000 INFO @ Sat, 17 Apr 2021 10:08:32: 3000000 INFO @ Sat, 17 Apr 2021 10:08:33: 7000000 INFO @ Sat, 17 Apr 2021 10:08:37: 12000000 INFO @ Sat, 17 Apr 2021 10:08:40: 4000000 INFO @ Sat, 17 Apr 2021 10:08:41: 8000000 INFO @ Sat, 17 Apr 2021 10:08:45: 13000000 INFO @ Sat, 17 Apr 2021 10:08:47: 5000000 INFO @ Sat, 17 Apr 2021 10:08:48: 9000000 INFO @ Sat, 17 Apr 2021 10:08:52: 14000000 INFO @ Sat, 17 Apr 2021 10:08:54: 6000000 INFO @ Sat, 17 Apr 2021 10:08:56: 10000000 INFO @ Sat, 17 Apr 2021 10:09:00: 15000000 INFO @ Sat, 17 Apr 2021 10:09:02: 7000000 INFO @ Sat, 17 Apr 2021 10:09:03: 11000000 INFO @ Sat, 17 Apr 2021 10:09:07: 16000000 INFO @ Sat, 17 Apr 2021 10:09:09: 8000000 INFO @ Sat, 17 Apr 2021 10:09:11: 12000000 INFO @ Sat, 17 Apr 2021 10:09:14: 17000000 INFO @ Sat, 17 Apr 2021 10:09:16: 9000000 INFO @ Sat, 17 Apr 2021 10:09:18: 13000000 INFO @ Sat, 17 Apr 2021 10:09:21: 18000000 INFO @ Sat, 17 Apr 2021 10:09:23: 10000000 INFO @ Sat, 17 Apr 2021 10:09:26: 14000000 INFO @ Sat, 17 Apr 2021 10:09:29: 19000000 INFO @ Sat, 17 Apr 2021 10:09:31: 11000000 INFO @ Sat, 17 Apr 2021 10:09:33: 15000000 INFO @ Sat, 17 Apr 2021 10:09:36: 20000000 INFO @ Sat, 17 Apr 2021 10:09:39: 12000000 INFO @ Sat, 17 Apr 2021 10:09:41: 16000000 INFO @ Sat, 17 Apr 2021 10:09:44: 21000000 INFO @ Sat, 17 Apr 2021 10:09:47: 13000000 INFO @ Sat, 17 Apr 2021 10:09:49: 17000000 INFO @ Sat, 17 Apr 2021 10:09:52: 22000000 INFO @ Sat, 17 Apr 2021 10:09:54: 14000000 INFO @ Sat, 17 Apr 2021 10:09:56: 18000000 INFO @ Sat, 17 Apr 2021 10:10:00: 23000000 INFO @ Sat, 17 Apr 2021 10:10:02: 15000000 INFO @ Sat, 17 Apr 2021 10:10:04: 19000000 INFO @ Sat, 17 Apr 2021 10:10:08: 24000000 INFO @ Sat, 17 Apr 2021 10:10:10: 16000000 INFO @ Sat, 17 Apr 2021 10:10:12: 20000000 INFO @ Sat, 17 Apr 2021 10:10:15: 25000000 INFO @ Sat, 17 Apr 2021 10:10:17: 17000000 INFO @ Sat, 17 Apr 2021 10:10:19: 21000000 INFO @ Sat, 17 Apr 2021 10:10:23: 26000000 INFO @ Sat, 17 Apr 2021 10:10:25: 18000000 INFO @ Sat, 17 Apr 2021 10:10:27: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 17 Apr 2021 10:10:28: #1 tag size is determined as 100 bps INFO @ Sat, 17 Apr 2021 10:10:28: #1 tag size = 100 INFO @ Sat, 17 Apr 2021 10:10:28: #1 total tags in treatment: 11846144 INFO @ Sat, 17 Apr 2021 10:10:28: #1 user defined the maximum tags... INFO @ Sat, 17 Apr 2021 10:10:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 17 Apr 2021 10:10:29: #1 tags after filtering in treatment: 6270139 INFO @ Sat, 17 Apr 2021 10:10:29: #1 Redundant rate of treatment: 0.47 INFO @ Sat, 17 Apr 2021 10:10:29: #1 finished! INFO @ Sat, 17 Apr 2021 10:10:29: #2 Build Peak Model... INFO @ Sat, 17 Apr 2021 10:10:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 17 Apr 2021 10:10:29: #2 number of paired peaks: 0 WARNING @ Sat, 17 Apr 2021 10:10:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 17 Apr 2021 10:10:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5811095/SRX5811095.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811095/SRX5811095.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811095/SRX5811095.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811095/SRX5811095.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 17 Apr 2021 10:10:32: 19000000 INFO @ Sat, 17 Apr 2021 10:10:34: 23000000 INFO @ Sat, 17 Apr 2021 10:10:40: 20000000 INFO @ Sat, 17 Apr 2021 10:10:42: 24000000 INFO @ Sat, 17 Apr 2021 10:10:47: 21000000 BigWig に変換しました。 INFO @ Sat, 17 Apr 2021 10:10:49: 25000000 INFO @ Sat, 17 Apr 2021 10:10:55: 22000000 INFO @ Sat, 17 Apr 2021 10:10:57: 26000000 INFO @ Sat, 17 Apr 2021 10:11:02: #1 tag size is determined as 100 bps INFO @ Sat, 17 Apr 2021 10:11:02: #1 tag size = 100 INFO @ Sat, 17 Apr 2021 10:11:02: #1 total tags in treatment: 11846144 INFO @ Sat, 17 Apr 2021 10:11:02: #1 user defined the maximum tags... INFO @ Sat, 17 Apr 2021 10:11:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 17 Apr 2021 10:11:02: 23000000 INFO @ Sat, 17 Apr 2021 10:11:02: #1 tags after filtering in treatment: 6270139 INFO @ Sat, 17 Apr 2021 10:11:02: #1 Redundant rate of treatment: 0.47 INFO @ Sat, 17 Apr 2021 10:11:02: #1 finished! INFO @ Sat, 17 Apr 2021 10:11:02: #2 Build Peak Model... INFO @ Sat, 17 Apr 2021 10:11:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 17 Apr 2021 10:11:03: #2 number of paired peaks: 0 WARNING @ Sat, 17 Apr 2021 10:11:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 17 Apr 2021 10:11:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5811095/SRX5811095.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811095/SRX5811095.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811095/SRX5811095.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811095/SRX5811095.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 17 Apr 2021 10:11:09: 24000000 INFO @ Sat, 17 Apr 2021 10:11:16: 25000000 INFO @ Sat, 17 Apr 2021 10:11:23: 26000000 INFO @ Sat, 17 Apr 2021 10:11:28: #1 tag size is determined as 100 bps INFO @ Sat, 17 Apr 2021 10:11:28: #1 tag size = 100 INFO @ Sat, 17 Apr 2021 10:11:28: #1 total tags in treatment: 11846144 INFO @ Sat, 17 Apr 2021 10:11:28: #1 user defined the maximum tags... INFO @ Sat, 17 Apr 2021 10:11:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 17 Apr 2021 10:11:28: #1 tags after filtering in treatment: 6270139 INFO @ Sat, 17 Apr 2021 10:11:28: #1 Redundant rate of treatment: 0.47 INFO @ Sat, 17 Apr 2021 10:11:28: #1 finished! INFO @ Sat, 17 Apr 2021 10:11:28: #2 Build Peak Model... INFO @ Sat, 17 Apr 2021 10:11:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 17 Apr 2021 10:11:29: #2 number of paired peaks: 0 WARNING @ Sat, 17 Apr 2021 10:11:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 17 Apr 2021 10:11:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5811095/SRX5811095.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811095/SRX5811095.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811095/SRX5811095.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811095/SRX5811095.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling