Job ID = 2641135 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 16,832,766 reads read : 16,832,766 reads written : 16,832,766 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:31 16832766 reads; of these: 16832766 (100.00%) were unpaired; of these: 1389527 (8.25%) aligned 0 times 9193485 (54.62%) aligned exactly 1 time 6249754 (37.13%) aligned >1 times 91.75% overall alignment rate Time searching: 00:02:31 Overall time: 00:02:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 8732794 / 15443239 = 0.5655 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 21:41:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX559604/SRX559604.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX559604/SRX559604.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX559604/SRX559604.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX559604/SRX559604.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:41:19: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:41:19: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:41:27: 1000000 INFO @ Sat, 24 Aug 2019 21:41:36: 2000000 INFO @ Sat, 24 Aug 2019 21:41:44: 3000000 INFO @ Sat, 24 Aug 2019 21:41:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX559604/SRX559604.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX559604/SRX559604.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX559604/SRX559604.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX559604/SRX559604.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:41:49: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:41:49: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:41:52: 4000000 INFO @ Sat, 24 Aug 2019 21:41:56: 1000000 INFO @ Sat, 24 Aug 2019 21:41:59: 5000000 INFO @ Sat, 24 Aug 2019 21:42:04: 2000000 INFO @ Sat, 24 Aug 2019 21:42:07: 6000000 INFO @ Sat, 24 Aug 2019 21:42:11: 3000000 INFO @ Sat, 24 Aug 2019 21:42:13: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:42:13: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:42:13: #1 total tags in treatment: 6710445 INFO @ Sat, 24 Aug 2019 21:42:13: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:42:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:42:13: #1 tags after filtering in treatment: 6710445 INFO @ Sat, 24 Aug 2019 21:42:13: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:42:13: #1 finished! INFO @ Sat, 24 Aug 2019 21:42:13: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:42:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:42:14: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:42:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:42:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX559604/SRX559604.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX559604/SRX559604.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX559604/SRX559604.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX559604/SRX559604.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... INFO @ Sat, 24 Aug 2019 21:42:18: 4000000 INFO @ Sat, 24 Aug 2019 21:42:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX559604/SRX559604.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX559604/SRX559604.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX559604/SRX559604.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX559604/SRX559604.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:42:19: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:42:19: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:42:24: 5000000 INFO @ Sat, 24 Aug 2019 21:42:28: 1000000 INFO @ Sat, 24 Aug 2019 21:42:31: 6000000 INFO @ Sat, 24 Aug 2019 21:42:36: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:42:36: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:42:36: #1 total tags in treatment: 6710445 INFO @ Sat, 24 Aug 2019 21:42:36: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:42:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:42:36: #1 tags after filtering in treatment: 6710445 INFO @ Sat, 24 Aug 2019 21:42:36: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:42:36: #1 finished! INFO @ Sat, 24 Aug 2019 21:42:36: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:42:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:42:37: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:42:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:42:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX559604/SRX559604.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX559604/SRX559604.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX559604/SRX559604.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX559604/SRX559604.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 21:42:37: 2000000 INFO @ Sat, 24 Aug 2019 21:42:46: 3000000 INFO @ Sat, 24 Aug 2019 21:42:54: 4000000 INFO @ Sat, 24 Aug 2019 21:43:02: 5000000 INFO @ Sat, 24 Aug 2019 21:43:10: 6000000 INFO @ Sat, 24 Aug 2019 21:43:16: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:43:16: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:43:16: #1 total tags in treatment: 6710445 INFO @ Sat, 24 Aug 2019 21:43:16: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:43:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:43:17: #1 tags after filtering in treatment: 6710445 INFO @ Sat, 24 Aug 2019 21:43:17: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:43:17: #1 finished! INFO @ Sat, 24 Aug 2019 21:43:17: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:43:17: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:43:17: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:43:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:43:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX559604/SRX559604.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX559604/SRX559604.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX559604/SRX559604.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX559604/SRX559604.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。