Job ID = 5790910 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 522,966 reads read : 1,045,932 reads written : 522,966 reads 0-length : 522,966 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:06 522966 reads; of these: 522966 (100.00%) were unpaired; of these: 153 (0.03%) aligned 0 times 465524 (89.02%) aligned exactly 1 time 57289 (10.95%) aligned >1 times 99.97% overall alignment rate Time searching: 00:00:06 Overall time: 00:00:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 40687 / 522813 = 0.0778 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:02:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5550836/SRX5550836.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5550836/SRX5550836.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5550836/SRX5550836.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5550836/SRX5550836.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:02:58: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:02:58: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:03:00: #1 tag size is determined as 76 bps INFO @ Wed, 22 Apr 2020 08:03:00: #1 tag size = 76 INFO @ Wed, 22 Apr 2020 08:03:00: #1 total tags in treatment: 482126 INFO @ Wed, 22 Apr 2020 08:03:00: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:03:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:03:00: #1 tags after filtering in treatment: 482126 INFO @ Wed, 22 Apr 2020 08:03:00: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 08:03:00: #1 finished! INFO @ Wed, 22 Apr 2020 08:03:00: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:03:00: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:03:00: #2 number of paired peaks: 343 WARNING @ Wed, 22 Apr 2020 08:03:00: Fewer paired peaks (343) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 343 pairs to build model! INFO @ Wed, 22 Apr 2020 08:03:00: start model_add_line... INFO @ Wed, 22 Apr 2020 08:03:00: start X-correlation... INFO @ Wed, 22 Apr 2020 08:03:00: end of X-cor INFO @ Wed, 22 Apr 2020 08:03:00: #2 finished! INFO @ Wed, 22 Apr 2020 08:03:00: #2 predicted fragment length is 108 bps INFO @ Wed, 22 Apr 2020 08:03:00: #2 alternative fragment length(s) may be 108 bps INFO @ Wed, 22 Apr 2020 08:03:00: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/SRX5550836/SRX5550836.05_model.r WARNING @ Wed, 22 Apr 2020 08:03:00: #2 Since the d (108) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Wed, 22 Apr 2020 08:03:00: #2 You may need to consider one of the other alternative d(s): 108 WARNING @ Wed, 22 Apr 2020 08:03:00: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Wed, 22 Apr 2020 08:03:00: #3 Call peaks... INFO @ Wed, 22 Apr 2020 08:03:00: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 22 Apr 2020 08:03:02: #3 Call peaks for each chromosome... INFO @ Wed, 22 Apr 2020 08:03:02: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/SRX5550836/SRX5550836.05_peaks.xls INFO @ Wed, 22 Apr 2020 08:03:02: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/SRX5550836/SRX5550836.05_peaks.narrowPeak INFO @ Wed, 22 Apr 2020 08:03:02: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/SRX5550836/SRX5550836.05_summits.bed INFO @ Wed, 22 Apr 2020 08:03:02: Done! pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (529 records, 4 fields): 2 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:03:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5550836/SRX5550836.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5550836/SRX5550836.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5550836/SRX5550836.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5550836/SRX5550836.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:03:28: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:03:28: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:03:31: #1 tag size is determined as 76 bps INFO @ Wed, 22 Apr 2020 08:03:31: #1 tag size = 76 INFO @ Wed, 22 Apr 2020 08:03:31: #1 total tags in treatment: 482126 INFO @ Wed, 22 Apr 2020 08:03:31: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:03:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:03:31: #1 tags after filtering in treatment: 482126 INFO @ Wed, 22 Apr 2020 08:03:31: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 08:03:31: #1 finished! INFO @ Wed, 22 Apr 2020 08:03:31: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:03:31: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:03:31: #2 number of paired peaks: 343 WARNING @ Wed, 22 Apr 2020 08:03:31: Fewer paired peaks (343) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 343 pairs to build model! INFO @ Wed, 22 Apr 2020 08:03:31: start model_add_line... INFO @ Wed, 22 Apr 2020 08:03:31: start X-correlation... INFO @ Wed, 22 Apr 2020 08:03:31: end of X-cor INFO @ Wed, 22 Apr 2020 08:03:31: #2 finished! INFO @ Wed, 22 Apr 2020 08:03:31: #2 predicted fragment length is 108 bps INFO @ Wed, 22 Apr 2020 08:03:31: #2 alternative fragment length(s) may be 108 bps INFO @ Wed, 22 Apr 2020 08:03:31: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/SRX5550836/SRX5550836.10_model.r WARNING @ Wed, 22 Apr 2020 08:03:31: #2 Since the d (108) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Wed, 22 Apr 2020 08:03:31: #2 You may need to consider one of the other alternative d(s): 108 WARNING @ Wed, 22 Apr 2020 08:03:31: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Wed, 22 Apr 2020 08:03:31: #3 Call peaks... INFO @ Wed, 22 Apr 2020 08:03:31: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 22 Apr 2020 08:03:32: #3 Call peaks for each chromosome... INFO @ Wed, 22 Apr 2020 08:03:33: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/SRX5550836/SRX5550836.10_peaks.xls INFO @ Wed, 22 Apr 2020 08:03:33: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/SRX5550836/SRX5550836.10_peaks.narrowPeak INFO @ Wed, 22 Apr 2020 08:03:33: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/SRX5550836/SRX5550836.10_summits.bed INFO @ Wed, 22 Apr 2020 08:03:33: Done! pass1 - making usageList (16 chroms): 0 millis pass2 - checking and writing primary data (315 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:03:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5550836/SRX5550836.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5550836/SRX5550836.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5550836/SRX5550836.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5550836/SRX5550836.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:03:58: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:03:58: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Apr 2020 08:04:02: #1 tag size is determined as 76 bps INFO @ Wed, 22 Apr 2020 08:04:02: #1 tag size = 76 INFO @ Wed, 22 Apr 2020 08:04:02: #1 total tags in treatment: 482126 INFO @ Wed, 22 Apr 2020 08:04:02: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:04:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:04:02: #1 tags after filtering in treatment: 482126 INFO @ Wed, 22 Apr 2020 08:04:02: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 08:04:02: #1 finished! INFO @ Wed, 22 Apr 2020 08:04:02: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:04:02: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:04:02: #2 number of paired peaks: 343 WARNING @ Wed, 22 Apr 2020 08:04:02: Fewer paired peaks (343) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 343 pairs to build model! INFO @ Wed, 22 Apr 2020 08:04:02: start model_add_line... INFO @ Wed, 22 Apr 2020 08:04:02: start X-correlation... INFO @ Wed, 22 Apr 2020 08:04:02: end of X-cor INFO @ Wed, 22 Apr 2020 08:04:02: #2 finished! INFO @ Wed, 22 Apr 2020 08:04:02: #2 predicted fragment length is 108 bps INFO @ Wed, 22 Apr 2020 08:04:02: #2 alternative fragment length(s) may be 108 bps INFO @ Wed, 22 Apr 2020 08:04:02: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/SRX5550836/SRX5550836.20_model.r WARNING @ Wed, 22 Apr 2020 08:04:02: #2 Since the d (108) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Wed, 22 Apr 2020 08:04:02: #2 You may need to consider one of the other alternative d(s): 108 WARNING @ Wed, 22 Apr 2020 08:04:02: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Wed, 22 Apr 2020 08:04:02: #3 Call peaks... INFO @ Wed, 22 Apr 2020 08:04:02: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Wed, 22 Apr 2020 08:04:04: #3 Call peaks for each chromosome... INFO @ Wed, 22 Apr 2020 08:04:04: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/SRX5550836/SRX5550836.20_peaks.xls INFO @ Wed, 22 Apr 2020 08:04:04: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/SRX5550836/SRX5550836.20_peaks.narrowPeak INFO @ Wed, 22 Apr 2020 08:04:04: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/SRX5550836/SRX5550836.20_summits.bed INFO @ Wed, 22 Apr 2020 08:04:04: Done! pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (128 records, 4 fields): 1 millis CompletedMACS2peakCalling