Job ID = 5790893 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-04-21T23:01:51 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 1,422,259 reads read : 2,844,518 reads written : 2,844,518 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:01:17 1422259 reads; of these: 1422259 (100.00%) were paired; of these: 4452 (0.31%) aligned concordantly 0 times 1132308 (79.61%) aligned concordantly exactly 1 time 285499 (20.07%) aligned concordantly >1 times ---- 4452 pairs aligned concordantly 0 times; of these: 297 (6.67%) aligned discordantly 1 time ---- 4155 pairs aligned 0 times concordantly or discordantly; of these: 8310 mates make up the pairs; of these: 4268 (51.36%) aligned 0 times 1048 (12.61%) aligned exactly 1 time 2994 (36.03%) aligned >1 times 99.85% overall alignment rate Time searching: 00:01:18 Overall time: 00:01:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 121794 / 1396263 = 0.0872 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:05:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5550825/SRX5550825.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5550825/SRX5550825.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5550825/SRX5550825.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5550825/SRX5550825.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:05:42: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:05:42: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:05:47: 1000000 INFO @ Wed, 22 Apr 2020 08:05:52: 2000000 INFO @ Wed, 22 Apr 2020 08:05:55: #1 tag size is determined as 76 bps INFO @ Wed, 22 Apr 2020 08:05:55: #1 tag size = 76 INFO @ Wed, 22 Apr 2020 08:05:55: #1 total tags in treatment: 1296195 INFO @ Wed, 22 Apr 2020 08:05:55: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:05:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:05:56: #1 tags after filtering in treatment: 1159946 INFO @ Wed, 22 Apr 2020 08:05:56: #1 Redundant rate of treatment: 0.11 INFO @ Wed, 22 Apr 2020 08:05:56: #1 finished! INFO @ Wed, 22 Apr 2020 08:05:56: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:05:56: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:05:56: #2 number of paired peaks: 41 WARNING @ Wed, 22 Apr 2020 08:05:56: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:05:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5550825/SRX5550825.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5550825/SRX5550825.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5550825/SRX5550825.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5550825/SRX5550825.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:06:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5550825/SRX5550825.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5550825/SRX5550825.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5550825/SRX5550825.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5550825/SRX5550825.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:06:11: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:06:11: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:06:16: 1000000 INFO @ Wed, 22 Apr 2020 08:06:21: 2000000 INFO @ Wed, 22 Apr 2020 08:06:24: #1 tag size is determined as 76 bps INFO @ Wed, 22 Apr 2020 08:06:24: #1 tag size = 76 INFO @ Wed, 22 Apr 2020 08:06:24: #1 total tags in treatment: 1296195 INFO @ Wed, 22 Apr 2020 08:06:24: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:06:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:06:24: #1 tags after filtering in treatment: 1159946 INFO @ Wed, 22 Apr 2020 08:06:24: #1 Redundant rate of treatment: 0.11 INFO @ Wed, 22 Apr 2020 08:06:24: #1 finished! INFO @ Wed, 22 Apr 2020 08:06:24: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:06:24: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:06:24: #2 number of paired peaks: 41 WARNING @ Wed, 22 Apr 2020 08:06:24: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:06:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5550825/SRX5550825.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5550825/SRX5550825.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5550825/SRX5550825.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5550825/SRX5550825.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:06:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5550825/SRX5550825.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5550825/SRX5550825.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5550825/SRX5550825.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5550825/SRX5550825.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:06:42: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:06:42: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:06:47: 1000000 INFO @ Wed, 22 Apr 2020 08:06:52: 2000000 INFO @ Wed, 22 Apr 2020 08:06:55: #1 tag size is determined as 76 bps INFO @ Wed, 22 Apr 2020 08:06:55: #1 tag size = 76 INFO @ Wed, 22 Apr 2020 08:06:55: #1 total tags in treatment: 1296195 INFO @ Wed, 22 Apr 2020 08:06:55: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:06:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:06:55: #1 tags after filtering in treatment: 1159946 INFO @ Wed, 22 Apr 2020 08:06:55: #1 Redundant rate of treatment: 0.11 INFO @ Wed, 22 Apr 2020 08:06:55: #1 finished! INFO @ Wed, 22 Apr 2020 08:06:55: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:06:55: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:06:55: #2 number of paired peaks: 41 WARNING @ Wed, 22 Apr 2020 08:06:55: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:06:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5550825/SRX5550825.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5550825/SRX5550825.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5550825/SRX5550825.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5550825/SRX5550825.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。