Job ID = 5790886 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 166,919 reads read : 333,838 reads written : 166,919 reads 0-length : 166,919 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:02 166919 reads; of these: 166919 (100.00%) were unpaired; of these: 48 (0.03%) aligned 0 times 150719 (90.29%) aligned exactly 1 time 16152 (9.68%) aligned >1 times 99.97% overall alignment rate Time searching: 00:00:02 Overall time: 00:00:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 4102 / 166871 = 0.0246 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:00:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5550819/SRX5550819.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5550819/SRX5550819.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5550819/SRX5550819.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5550819/SRX5550819.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:00:58: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:00:58: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:00:59: #1 tag size is determined as 76 bps INFO @ Wed, 22 Apr 2020 08:00:59: #1 tag size = 76 INFO @ Wed, 22 Apr 2020 08:00:59: #1 total tags in treatment: 162769 INFO @ Wed, 22 Apr 2020 08:00:59: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:00:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:00:59: #1 tags after filtering in treatment: 162769 INFO @ Wed, 22 Apr 2020 08:00:59: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 08:00:59: #1 finished! INFO @ Wed, 22 Apr 2020 08:00:59: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:00:59: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:00:59: #2 number of paired peaks: 27 WARNING @ Wed, 22 Apr 2020 08:00:59: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:00:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5550819/SRX5550819.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5550819/SRX5550819.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5550819/SRX5550819.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5550819/SRX5550819.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:01:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5550819/SRX5550819.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5550819/SRX5550819.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5550819/SRX5550819.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5550819/SRX5550819.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:01:28: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:01:28: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:01:29: #1 tag size is determined as 76 bps INFO @ Wed, 22 Apr 2020 08:01:29: #1 tag size = 76 INFO @ Wed, 22 Apr 2020 08:01:29: #1 total tags in treatment: 162769 INFO @ Wed, 22 Apr 2020 08:01:29: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:01:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:01:29: #1 tags after filtering in treatment: 162769 INFO @ Wed, 22 Apr 2020 08:01:29: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 08:01:29: #1 finished! INFO @ Wed, 22 Apr 2020 08:01:29: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:01:29: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:01:29: #2 number of paired peaks: 27 WARNING @ Wed, 22 Apr 2020 08:01:29: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:01:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5550819/SRX5550819.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5550819/SRX5550819.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5550819/SRX5550819.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5550819/SRX5550819.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Wed, 22 Apr 2020 08:01:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5550819/SRX5550819.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5550819/SRX5550819.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5550819/SRX5550819.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5550819/SRX5550819.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:01:58: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:01:58: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:01:59: #1 tag size is determined as 76 bps INFO @ Wed, 22 Apr 2020 08:01:59: #1 tag size = 76 INFO @ Wed, 22 Apr 2020 08:01:59: #1 total tags in treatment: 162769 INFO @ Wed, 22 Apr 2020 08:01:59: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:01:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:01:59: #1 tags after filtering in treatment: 162769 INFO @ Wed, 22 Apr 2020 08:01:59: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 08:01:59: #1 finished! INFO @ Wed, 22 Apr 2020 08:01:59: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:01:59: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:01:59: #2 number of paired peaks: 27 WARNING @ Wed, 22 Apr 2020 08:01:59: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:01:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5550819/SRX5550819.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5550819/SRX5550819.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5550819/SRX5550819.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5550819/SRX5550819.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling