Job ID = 4289125 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 21,967,446 reads read : 43,934,892 reads written : 21,967,446 reads 0-length : 21,967,446 spots read : 27,462,873 reads read : 54,925,746 reads written : 27,462,873 reads 0-length : 27,462,873 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:11 49430319 reads; of these: 49430319 (100.00%) were unpaired; of these: 31108342 (62.93%) aligned 0 times 14828931 (30.00%) aligned exactly 1 time 3493046 (7.07%) aligned >1 times 37.07% overall alignment rate Time searching: 00:07:11 Overall time: 00:07:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 9253679 / 18321977 = 0.5051 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Tue, 10 Dec 2019 13:58:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5486252/SRX5486252.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5486252/SRX5486252.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5486252/SRX5486252.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5486252/SRX5486252.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 13:58:18: #1 read tag files... INFO @ Tue, 10 Dec 2019 13:58:18: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 13:58:30: 1000000 INFO @ Tue, 10 Dec 2019 13:58:41: 2000000 INFO @ Tue, 10 Dec 2019 13:58:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5486252/SRX5486252.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5486252/SRX5486252.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5486252/SRX5486252.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5486252/SRX5486252.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 13:58:47: #1 read tag files... INFO @ Tue, 10 Dec 2019 13:58:47: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 13:58:51: 3000000 INFO @ Tue, 10 Dec 2019 13:58:58: 1000000 INFO @ Tue, 10 Dec 2019 13:59:03: 4000000 INFO @ Tue, 10 Dec 2019 13:59:08: 2000000 INFO @ Tue, 10 Dec 2019 13:59:14: 5000000 BedGraph に変換中... INFO @ Tue, 10 Dec 2019 13:59:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5486252/SRX5486252.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5486252/SRX5486252.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5486252/SRX5486252.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5486252/SRX5486252.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 13:59:17: #1 read tag files... INFO @ Tue, 10 Dec 2019 13:59:17: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 13:59:18: 3000000 INFO @ Tue, 10 Dec 2019 13:59:25: 6000000 INFO @ Tue, 10 Dec 2019 13:59:28: 4000000 INFO @ Tue, 10 Dec 2019 13:59:30: 1000000 INFO @ Tue, 10 Dec 2019 13:59:37: 7000000 INFO @ Tue, 10 Dec 2019 13:59:38: 5000000 INFO @ Tue, 10 Dec 2019 13:59:41: 2000000 INFO @ Tue, 10 Dec 2019 13:59:48: 6000000 INFO @ Tue, 10 Dec 2019 13:59:49: 8000000 INFO @ Tue, 10 Dec 2019 13:59:53: 3000000 INFO @ Tue, 10 Dec 2019 13:59:57: 7000000 INFO @ Tue, 10 Dec 2019 14:00:00: 9000000 INFO @ Tue, 10 Dec 2019 14:00:01: #1 tag size is determined as 50 bps INFO @ Tue, 10 Dec 2019 14:00:01: #1 tag size = 50 INFO @ Tue, 10 Dec 2019 14:00:01: #1 total tags in treatment: 9068298 INFO @ Tue, 10 Dec 2019 14:00:01: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:00:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:00:01: #1 tags after filtering in treatment: 9068298 INFO @ Tue, 10 Dec 2019 14:00:01: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:00:01: #1 finished! INFO @ Tue, 10 Dec 2019 14:00:01: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:00:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:00:02: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 14:00:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:00:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5486252/SRX5486252.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5486252/SRX5486252.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5486252/SRX5486252.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5486252/SRX5486252.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 14:00:04: 4000000 INFO @ Tue, 10 Dec 2019 14:00:07: 8000000 INFO @ Tue, 10 Dec 2019 14:00:15: 5000000 INFO @ Tue, 10 Dec 2019 14:00:17: 9000000 INFO @ Tue, 10 Dec 2019 14:00:18: #1 tag size is determined as 50 bps INFO @ Tue, 10 Dec 2019 14:00:18: #1 tag size = 50 INFO @ Tue, 10 Dec 2019 14:00:18: #1 total tags in treatment: 9068298 INFO @ Tue, 10 Dec 2019 14:00:18: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:00:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:00:18: #1 tags after filtering in treatment: 9068298 INFO @ Tue, 10 Dec 2019 14:00:18: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:00:18: #1 finished! INFO @ Tue, 10 Dec 2019 14:00:18: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:00:18: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:00:19: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 14:00:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:00:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5486252/SRX5486252.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5486252/SRX5486252.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5486252/SRX5486252.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5486252/SRX5486252.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 14:00:25: 6000000 INFO @ Tue, 10 Dec 2019 14:00:36: 7000000 INFO @ Tue, 10 Dec 2019 14:00:47: 8000000 INFO @ Tue, 10 Dec 2019 14:00:58: 9000000 INFO @ Tue, 10 Dec 2019 14:00:58: #1 tag size is determined as 50 bps INFO @ Tue, 10 Dec 2019 14:00:58: #1 tag size = 50 INFO @ Tue, 10 Dec 2019 14:00:58: #1 total tags in treatment: 9068298 INFO @ Tue, 10 Dec 2019 14:00:58: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:00:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:00:59: #1 tags after filtering in treatment: 9068298 INFO @ Tue, 10 Dec 2019 14:00:59: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:00:59: #1 finished! INFO @ Tue, 10 Dec 2019 14:00:59: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:00:59: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:00:59: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 14:00:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:00:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5486252/SRX5486252.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5486252/SRX5486252.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5486252/SRX5486252.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5486252/SRX5486252.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。