Job ID = 4289115 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 12,104,159 reads read : 24,208,318 reads written : 12,104,159 reads 0-length : 12,104,159 spots read : 6,677,213 reads read : 13,354,426 reads written : 6,677,213 reads 0-length : 6,677,213 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:56 18781372 reads; of these: 18781372 (100.00%) were unpaired; of these: 9517040 (50.67%) aligned 0 times 6854941 (36.50%) aligned exactly 1 time 2409391 (12.83%) aligned >1 times 49.33% overall alignment rate Time searching: 00:02:56 Overall time: 00:02:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 3986839 / 9264332 = 0.4303 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Tue, 10 Dec 2019 13:42:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5486250/SRX5486250.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5486250/SRX5486250.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5486250/SRX5486250.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5486250/SRX5486250.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 13:42:24: #1 read tag files... INFO @ Tue, 10 Dec 2019 13:42:24: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 13:42:31: 1000000 INFO @ Tue, 10 Dec 2019 13:42:38: 2000000 INFO @ Tue, 10 Dec 2019 13:42:45: 3000000 INFO @ Tue, 10 Dec 2019 13:42:52: 4000000 INFO @ Tue, 10 Dec 2019 13:42:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5486250/SRX5486250.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5486250/SRX5486250.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5486250/SRX5486250.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5486250/SRX5486250.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 13:42:54: #1 read tag files... INFO @ Tue, 10 Dec 2019 13:42:54: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 13:42:59: 5000000 INFO @ Tue, 10 Dec 2019 13:43:01: #1 tag size is determined as 50 bps INFO @ Tue, 10 Dec 2019 13:43:01: #1 tag size = 50 INFO @ Tue, 10 Dec 2019 13:43:01: #1 total tags in treatment: 5277493 INFO @ Tue, 10 Dec 2019 13:43:01: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 13:43:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 13:43:01: #1 tags after filtering in treatment: 5277493 INFO @ Tue, 10 Dec 2019 13:43:01: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 13:43:01: #1 finished! INFO @ Tue, 10 Dec 2019 13:43:01: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 13:43:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 13:43:01: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 13:43:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 13:43:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5486250/SRX5486250.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5486250/SRX5486250.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5486250/SRX5486250.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5486250/SRX5486250.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 13:43:03: 1000000 INFO @ Tue, 10 Dec 2019 13:43:11: 2000000 INFO @ Tue, 10 Dec 2019 13:43:19: 3000000 BedGraph に変換中... INFO @ Tue, 10 Dec 2019 13:43:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5486250/SRX5486250.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5486250/SRX5486250.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5486250/SRX5486250.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5486250/SRX5486250.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 13:43:24: #1 read tag files... INFO @ Tue, 10 Dec 2019 13:43:24: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 13:43:28: 4000000 INFO @ Tue, 10 Dec 2019 13:43:31: 1000000 INFO @ Tue, 10 Dec 2019 13:43:36: 5000000 INFO @ Tue, 10 Dec 2019 13:43:38: #1 tag size is determined as 50 bps INFO @ Tue, 10 Dec 2019 13:43:38: #1 tag size = 50 INFO @ Tue, 10 Dec 2019 13:43:38: #1 total tags in treatment: 5277493 INFO @ Tue, 10 Dec 2019 13:43:38: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 13:43:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 13:43:38: #1 tags after filtering in treatment: 5277493 INFO @ Tue, 10 Dec 2019 13:43:38: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 13:43:38: #1 finished! INFO @ Tue, 10 Dec 2019 13:43:38: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 13:43:38: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 13:43:39: 2000000 INFO @ Tue, 10 Dec 2019 13:43:39: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 13:43:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 13:43:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5486250/SRX5486250.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5486250/SRX5486250.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5486250/SRX5486250.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5486250/SRX5486250.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 13:43:46: 3000000 INFO @ Tue, 10 Dec 2019 13:43:52: 4000000 INFO @ Tue, 10 Dec 2019 13:43:59: 5000000 INFO @ Tue, 10 Dec 2019 13:44:01: #1 tag size is determined as 50 bps INFO @ Tue, 10 Dec 2019 13:44:01: #1 tag size = 50 INFO @ Tue, 10 Dec 2019 13:44:01: #1 total tags in treatment: 5277493 INFO @ Tue, 10 Dec 2019 13:44:01: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 13:44:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 13:44:01: #1 tags after filtering in treatment: 5277493 INFO @ Tue, 10 Dec 2019 13:44:01: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 13:44:01: #1 finished! INFO @ Tue, 10 Dec 2019 13:44:01: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 13:44:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 13:44:02: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 13:44:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 13:44:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5486250/SRX5486250.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5486250/SRX5486250.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5486250/SRX5486250.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5486250/SRX5486250.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。