Job ID = 2641104 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 68,325,409 reads read : 68,325,409 reads written : 68,325,409 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:59 68325409 reads; of these: 68325409 (100.00%) were unpaired; of these: 3364602 (4.92%) aligned 0 times 54985496 (80.48%) aligned exactly 1 time 9975311 (14.60%) aligned >1 times 95.08% overall alignment rate Time searching: 00:11:59 Overall time: 00:11:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 47964157 / 64960807 = 0.7384 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 22:09:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5431205/SRX5431205.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5431205/SRX5431205.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5431205/SRX5431205.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5431205/SRX5431205.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 22:09:30: #1 read tag files... INFO @ Sat, 24 Aug 2019 22:09:30: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 22:09:39: 1000000 INFO @ Sat, 24 Aug 2019 22:09:47: 2000000 INFO @ Sat, 24 Aug 2019 22:09:55: 3000000 INFO @ Sat, 24 Aug 2019 22:10:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5431205/SRX5431205.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5431205/SRX5431205.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5431205/SRX5431205.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5431205/SRX5431205.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 22:10:01: #1 read tag files... INFO @ Sat, 24 Aug 2019 22:10:01: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 22:10:05: 4000000 INFO @ Sat, 24 Aug 2019 22:10:10: 1000000 INFO @ Sat, 24 Aug 2019 22:10:14: 5000000 INFO @ Sat, 24 Aug 2019 22:10:19: 2000000 INFO @ Sat, 24 Aug 2019 22:10:23: 6000000 INFO @ Sat, 24 Aug 2019 22:10:28: 3000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 22:10:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5431205/SRX5431205.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5431205/SRX5431205.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5431205/SRX5431205.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5431205/SRX5431205.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 22:10:31: #1 read tag files... INFO @ Sat, 24 Aug 2019 22:10:31: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 22:10:31: 7000000 INFO @ Sat, 24 Aug 2019 22:10:40: 8000000 INFO @ Sat, 24 Aug 2019 22:10:41: 4000000 INFO @ Sat, 24 Aug 2019 22:10:42: 1000000 INFO @ Sat, 24 Aug 2019 22:10:48: 9000000 INFO @ Sat, 24 Aug 2019 22:10:52: 2000000 INFO @ Sat, 24 Aug 2019 22:10:53: 5000000 INFO @ Sat, 24 Aug 2019 22:10:57: 10000000 INFO @ Sat, 24 Aug 2019 22:11:03: 3000000 INFO @ Sat, 24 Aug 2019 22:11:06: 11000000 INFO @ Sat, 24 Aug 2019 22:11:06: 6000000 INFO @ Sat, 24 Aug 2019 22:11:14: 12000000 INFO @ Sat, 24 Aug 2019 22:11:17: 4000000 INFO @ Sat, 24 Aug 2019 22:11:18: 7000000 INFO @ Sat, 24 Aug 2019 22:11:23: 13000000 INFO @ Sat, 24 Aug 2019 22:11:29: 5000000 INFO @ Sat, 24 Aug 2019 22:11:29: 8000000 INFO @ Sat, 24 Aug 2019 22:11:33: 14000000 INFO @ Sat, 24 Aug 2019 22:11:40: 6000000 INFO @ Sat, 24 Aug 2019 22:11:40: 9000000 INFO @ Sat, 24 Aug 2019 22:11:41: 15000000 INFO @ Sat, 24 Aug 2019 22:11:50: 16000000 INFO @ Sat, 24 Aug 2019 22:11:51: 7000000 INFO @ Sat, 24 Aug 2019 22:11:51: 10000000 INFO @ Sat, 24 Aug 2019 22:11:59: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 22:11:59: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 22:11:59: #1 total tags in treatment: 16996650 INFO @ Sat, 24 Aug 2019 22:11:59: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 22:11:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 22:11:59: #1 tags after filtering in treatment: 16996650 INFO @ Sat, 24 Aug 2019 22:11:59: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 22:11:59: #1 finished! INFO @ Sat, 24 Aug 2019 22:11:59: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 22:11:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 22:12:00: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 22:12:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 22:12:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5431205/SRX5431205.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431205/SRX5431205.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431205/SRX5431205.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431205/SRX5431205.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 22:12:02: 11000000 INFO @ Sat, 24 Aug 2019 22:12:02: 8000000 INFO @ Sat, 24 Aug 2019 22:12:13: 12000000 INFO @ Sat, 24 Aug 2019 22:12:14: 9000000 INFO @ Sat, 24 Aug 2019 22:12:24: 10000000 INFO @ Sat, 24 Aug 2019 22:12:24: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 24 Aug 2019 22:12:35: 11000000 INFO @ Sat, 24 Aug 2019 22:12:35: 14000000 BigWig に変換しました。 INFO @ Sat, 24 Aug 2019 22:12:46: 12000000 INFO @ Sat, 24 Aug 2019 22:12:47: 15000000 INFO @ Sat, 24 Aug 2019 22:12:57: 16000000 INFO @ Sat, 24 Aug 2019 22:12:57: 13000000 INFO @ Sat, 24 Aug 2019 22:13:08: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 22:13:08: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 22:13:08: #1 total tags in treatment: 16996650 INFO @ Sat, 24 Aug 2019 22:13:08: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 22:13:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 22:13:08: #1 tags after filtering in treatment: 16996650 INFO @ Sat, 24 Aug 2019 22:13:08: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 22:13:08: #1 finished! INFO @ Sat, 24 Aug 2019 22:13:08: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 22:13:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 22:13:08: 14000000 INFO @ Sat, 24 Aug 2019 22:13:09: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 22:13:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 22:13:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5431205/SRX5431205.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431205/SRX5431205.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431205/SRX5431205.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431205/SRX5431205.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 22:13:17: 15000000 INFO @ Sat, 24 Aug 2019 22:13:27: 16000000 INFO @ Sat, 24 Aug 2019 22:13:36: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 22:13:36: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 22:13:36: #1 total tags in treatment: 16996650 INFO @ Sat, 24 Aug 2019 22:13:36: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 22:13:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 22:13:36: #1 tags after filtering in treatment: 16996650 INFO @ Sat, 24 Aug 2019 22:13:36: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 22:13:36: #1 finished! INFO @ Sat, 24 Aug 2019 22:13:36: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 22:13:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 22:13:37: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 22:13:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 22:13:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5431205/SRX5431205.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431205/SRX5431205.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431205/SRX5431205.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431205/SRX5431205.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling