Job ID = 2641100 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 86,688,328 reads read : 86,688,328 reads written : 86,688,328 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:53 86688328 reads; of these: 86688328 (100.00%) were unpaired; of these: 2040792 (2.35%) aligned 0 times 69984617 (80.73%) aligned exactly 1 time 14662919 (16.91%) aligned >1 times 97.65% overall alignment rate Time searching: 00:14:53 Overall time: 00:14:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 36 files... [bam_rmdupse_core] 62538793 / 84647536 = 0.7388 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 22:19:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5431201/SRX5431201.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5431201/SRX5431201.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5431201/SRX5431201.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5431201/SRX5431201.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 22:19:56: #1 read tag files... INFO @ Sat, 24 Aug 2019 22:19:56: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 22:20:02: 1000000 INFO @ Sat, 24 Aug 2019 22:20:08: 2000000 INFO @ Sat, 24 Aug 2019 22:20:14: 3000000 INFO @ Sat, 24 Aug 2019 22:20:20: 4000000 INFO @ Sat, 24 Aug 2019 22:20:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5431201/SRX5431201.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5431201/SRX5431201.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5431201/SRX5431201.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5431201/SRX5431201.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 22:20:25: #1 read tag files... INFO @ Sat, 24 Aug 2019 22:20:25: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 22:20:26: 5000000 INFO @ Sat, 24 Aug 2019 22:20:32: 6000000 INFO @ Sat, 24 Aug 2019 22:20:33: 1000000 INFO @ Sat, 24 Aug 2019 22:20:39: 7000000 INFO @ Sat, 24 Aug 2019 22:20:40: 2000000 INFO @ Sat, 24 Aug 2019 22:20:45: 8000000 INFO @ Sat, 24 Aug 2019 22:20:47: 3000000 INFO @ Sat, 24 Aug 2019 22:20:51: 9000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 22:20:54: 4000000 INFO @ Sat, 24 Aug 2019 22:20:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5431201/SRX5431201.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5431201/SRX5431201.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5431201/SRX5431201.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5431201/SRX5431201.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 22:20:55: #1 read tag files... INFO @ Sat, 24 Aug 2019 22:20:55: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 22:20:58: 10000000 INFO @ Sat, 24 Aug 2019 22:21:01: 5000000 INFO @ Sat, 24 Aug 2019 22:21:02: 1000000 INFO @ Sat, 24 Aug 2019 22:21:04: 11000000 INFO @ Sat, 24 Aug 2019 22:21:08: 6000000 INFO @ Sat, 24 Aug 2019 22:21:09: 2000000 INFO @ Sat, 24 Aug 2019 22:21:11: 12000000 INFO @ Sat, 24 Aug 2019 22:21:15: 7000000 INFO @ Sat, 24 Aug 2019 22:21:16: 3000000 INFO @ Sat, 24 Aug 2019 22:21:18: 13000000 INFO @ Sat, 24 Aug 2019 22:21:21: 8000000 INFO @ Sat, 24 Aug 2019 22:21:23: 4000000 INFO @ Sat, 24 Aug 2019 22:21:24: 14000000 INFO @ Sat, 24 Aug 2019 22:21:28: 9000000 INFO @ Sat, 24 Aug 2019 22:21:29: 5000000 INFO @ Sat, 24 Aug 2019 22:21:31: 15000000 INFO @ Sat, 24 Aug 2019 22:21:35: 10000000 INFO @ Sat, 24 Aug 2019 22:21:36: 6000000 INFO @ Sat, 24 Aug 2019 22:21:38: 16000000 INFO @ Sat, 24 Aug 2019 22:21:42: 11000000 INFO @ Sat, 24 Aug 2019 22:21:43: 7000000 INFO @ Sat, 24 Aug 2019 22:21:45: 17000000 INFO @ Sat, 24 Aug 2019 22:21:48: 12000000 INFO @ Sat, 24 Aug 2019 22:21:50: 8000000 INFO @ Sat, 24 Aug 2019 22:21:51: 18000000 INFO @ Sat, 24 Aug 2019 22:21:55: 13000000 INFO @ Sat, 24 Aug 2019 22:21:57: 9000000 INFO @ Sat, 24 Aug 2019 22:21:58: 19000000 INFO @ Sat, 24 Aug 2019 22:22:02: 14000000 INFO @ Sat, 24 Aug 2019 22:22:04: 10000000 INFO @ Sat, 24 Aug 2019 22:22:05: 20000000 INFO @ Sat, 24 Aug 2019 22:22:09: 15000000 INFO @ Sat, 24 Aug 2019 22:22:10: 11000000 INFO @ Sat, 24 Aug 2019 22:22:11: 21000000 INFO @ Sat, 24 Aug 2019 22:22:15: 16000000 INFO @ Sat, 24 Aug 2019 22:22:17: 12000000 INFO @ Sat, 24 Aug 2019 22:22:17: 22000000 INFO @ Sat, 24 Aug 2019 22:22:18: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 22:22:18: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 22:22:18: #1 total tags in treatment: 22108743 INFO @ Sat, 24 Aug 2019 22:22:18: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 22:22:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 22:22:19: #1 tags after filtering in treatment: 22108743 INFO @ Sat, 24 Aug 2019 22:22:19: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 22:22:19: #1 finished! INFO @ Sat, 24 Aug 2019 22:22:19: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 22:22:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 22:22:20: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 22:22:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 22:22:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5431201/SRX5431201.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431201/SRX5431201.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431201/SRX5431201.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431201/SRX5431201.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 22:22:22: 17000000 INFO @ Sat, 24 Aug 2019 22:22:23: 13000000 INFO @ Sat, 24 Aug 2019 22:22:28: 18000000 INFO @ Sat, 24 Aug 2019 22:22:29: 14000000 INFO @ Sat, 24 Aug 2019 22:22:35: 19000000 INFO @ Sat, 24 Aug 2019 22:22:35: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 24 Aug 2019 22:22:41: 16000000 INFO @ Sat, 24 Aug 2019 22:22:41: 20000000 BigWig に変換しました。 INFO @ Sat, 24 Aug 2019 22:22:47: 17000000 INFO @ Sat, 24 Aug 2019 22:22:47: 21000000 INFO @ Sat, 24 Aug 2019 22:22:53: 18000000 INFO @ Sat, 24 Aug 2019 22:22:54: 22000000 INFO @ Sat, 24 Aug 2019 22:22:55: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 22:22:55: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 22:22:55: #1 total tags in treatment: 22108743 INFO @ Sat, 24 Aug 2019 22:22:55: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 22:22:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 22:22:55: #1 tags after filtering in treatment: 22108743 INFO @ Sat, 24 Aug 2019 22:22:55: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 22:22:55: #1 finished! INFO @ Sat, 24 Aug 2019 22:22:55: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 22:22:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 22:22:57: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 22:22:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 22:22:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5431201/SRX5431201.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431201/SRX5431201.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431201/SRX5431201.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431201/SRX5431201.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 22:22:59: 19000000 INFO @ Sat, 24 Aug 2019 22:23:05: 20000000 INFO @ Sat, 24 Aug 2019 22:23:11: 21000000 INFO @ Sat, 24 Aug 2019 22:23:17: 22000000 INFO @ Sat, 24 Aug 2019 22:23:18: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 22:23:18: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 22:23:18: #1 total tags in treatment: 22108743 INFO @ Sat, 24 Aug 2019 22:23:18: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 22:23:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 22:23:19: #1 tags after filtering in treatment: 22108743 INFO @ Sat, 24 Aug 2019 22:23:19: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 22:23:19: #1 finished! INFO @ Sat, 24 Aug 2019 22:23:19: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 22:23:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 22:23:20: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 22:23:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 22:23:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5431201/SRX5431201.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431201/SRX5431201.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431201/SRX5431201.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431201/SRX5431201.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling