Job ID = 2641098 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 64,283,355 reads read : 64,283,355 reads written : 64,283,355 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:18 64283355 reads; of these: 64283355 (100.00%) were unpaired; of these: 64255021 (99.96%) aligned 0 times 4348 (0.01%) aligned exactly 1 time 23986 (0.04%) aligned >1 times 0.04% overall alignment rate Time searching: 00:05:18 Overall time: 00:05:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 22195 / 28334 = 0.7833 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 21:46:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5431199/SRX5431199.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5431199/SRX5431199.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5431199/SRX5431199.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5431199/SRX5431199.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:46:33: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:46:33: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:46:33: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:46:33: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:46:33: #1 total tags in treatment: 6139 INFO @ Sat, 24 Aug 2019 21:46:33: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:46:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:46:33: #1 tags after filtering in treatment: 6139 INFO @ Sat, 24 Aug 2019 21:46:33: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:46:33: #1 finished! INFO @ Sat, 24 Aug 2019 21:46:33: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:46:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:46:33: #2 number of paired peaks: 47 WARNING @ Sat, 24 Aug 2019 21:46:33: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:46:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5431199/SRX5431199.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431199/SRX5431199.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431199/SRX5431199.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431199/SRX5431199.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 21:47:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5431199/SRX5431199.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5431199/SRX5431199.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5431199/SRX5431199.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5431199/SRX5431199.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:47:03: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:47:03: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:47:03: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:47:03: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:47:03: #1 total tags in treatment: 6139 INFO @ Sat, 24 Aug 2019 21:47:03: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:47:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:47:03: #1 tags after filtering in treatment: 6139 INFO @ Sat, 24 Aug 2019 21:47:03: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:47:03: #1 finished! INFO @ Sat, 24 Aug 2019 21:47:03: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:47:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:47:03: #2 number of paired peaks: 47 WARNING @ Sat, 24 Aug 2019 21:47:03: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:47:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5431199/SRX5431199.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431199/SRX5431199.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431199/SRX5431199.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431199/SRX5431199.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sat, 24 Aug 2019 21:47:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5431199/SRX5431199.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5431199/SRX5431199.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5431199/SRX5431199.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5431199/SRX5431199.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:47:33: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:47:33: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:47:33: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:47:33: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:47:33: #1 total tags in treatment: 6139 INFO @ Sat, 24 Aug 2019 21:47:33: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:47:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:47:33: #1 tags after filtering in treatment: 6139 INFO @ Sat, 24 Aug 2019 21:47:33: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:47:33: #1 finished! INFO @ Sat, 24 Aug 2019 21:47:33: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:47:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:47:33: #2 number of paired peaks: 47 WARNING @ Sat, 24 Aug 2019 21:47:33: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:47:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5431199/SRX5431199.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431199/SRX5431199.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431199/SRX5431199.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431199/SRX5431199.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling