Job ID = 2641093 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 86,342,580 reads read : 86,342,580 reads written : 86,342,580 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:48 86342580 reads; of these: 86342580 (100.00%) were unpaired; of these: 28636357 (33.17%) aligned 0 times 51934676 (60.15%) aligned exactly 1 time 5771547 (6.68%) aligned >1 times 66.83% overall alignment rate Time searching: 00:11:48 Overall time: 00:11:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 46109715 / 57706223 = 0.7990 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 22:14:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5431196/SRX5431196.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5431196/SRX5431196.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5431196/SRX5431196.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5431196/SRX5431196.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 22:14:12: #1 read tag files... INFO @ Sat, 24 Aug 2019 22:14:12: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 22:14:20: 1000000 INFO @ Sat, 24 Aug 2019 22:14:27: 2000000 INFO @ Sat, 24 Aug 2019 22:14:34: 3000000 INFO @ Sat, 24 Aug 2019 22:14:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5431196/SRX5431196.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5431196/SRX5431196.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5431196/SRX5431196.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5431196/SRX5431196.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 22:14:41: #1 read tag files... INFO @ Sat, 24 Aug 2019 22:14:41: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 22:14:42: 4000000 INFO @ Sat, 24 Aug 2019 22:14:49: 5000000 INFO @ Sat, 24 Aug 2019 22:14:49: 1000000 INFO @ Sat, 24 Aug 2019 22:14:56: 6000000 INFO @ Sat, 24 Aug 2019 22:14:57: 2000000 INFO @ Sat, 24 Aug 2019 22:15:04: 7000000 INFO @ Sat, 24 Aug 2019 22:15:05: 3000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 22:15:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5431196/SRX5431196.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5431196/SRX5431196.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5431196/SRX5431196.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5431196/SRX5431196.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 22:15:11: #1 read tag files... INFO @ Sat, 24 Aug 2019 22:15:11: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 22:15:11: 8000000 INFO @ Sat, 24 Aug 2019 22:15:12: 4000000 INFO @ Sat, 24 Aug 2019 22:15:19: 9000000 INFO @ Sat, 24 Aug 2019 22:15:19: 1000000 INFO @ Sat, 24 Aug 2019 22:15:20: 5000000 INFO @ Sat, 24 Aug 2019 22:15:26: 10000000 INFO @ Sat, 24 Aug 2019 22:15:27: 2000000 INFO @ Sat, 24 Aug 2019 22:15:27: 6000000 INFO @ Sat, 24 Aug 2019 22:15:34: 11000000 INFO @ Sat, 24 Aug 2019 22:15:35: 3000000 INFO @ Sat, 24 Aug 2019 22:15:35: 7000000 INFO @ Sat, 24 Aug 2019 22:15:38: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 22:15:38: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 22:15:38: #1 total tags in treatment: 11596508 INFO @ Sat, 24 Aug 2019 22:15:38: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 22:15:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 22:15:39: #1 tags after filtering in treatment: 11596508 INFO @ Sat, 24 Aug 2019 22:15:39: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 22:15:39: #1 finished! INFO @ Sat, 24 Aug 2019 22:15:39: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 22:15:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 22:15:39: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 22:15:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 22:15:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5431196/SRX5431196.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431196/SRX5431196.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431196/SRX5431196.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431196/SRX5431196.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 22:15:42: 4000000 INFO @ Sat, 24 Aug 2019 22:15:43: 8000000 INFO @ Sat, 24 Aug 2019 22:15:50: 5000000 INFO @ Sat, 24 Aug 2019 22:15:50: 9000000 INFO @ Sat, 24 Aug 2019 22:15:58: 6000000 INFO @ Sat, 24 Aug 2019 22:15:58: 10000000 INFO @ Sat, 24 Aug 2019 22:16:05: 7000000 INFO @ Sat, 24 Aug 2019 22:16:05: 11000000 INFO @ Sat, 24 Aug 2019 22:16:09: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 22:16:09: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 22:16:09: #1 total tags in treatment: 11596508 INFO @ Sat, 24 Aug 2019 22:16:09: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 22:16:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 22:16:10: #1 tags after filtering in treatment: 11596508 INFO @ Sat, 24 Aug 2019 22:16:10: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 22:16:10: #1 finished! INFO @ Sat, 24 Aug 2019 22:16:10: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 22:16:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 22:16:11: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 22:16:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 22:16:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5431196/SRX5431196.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431196/SRX5431196.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431196/SRX5431196.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431196/SRX5431196.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 22:16:13: 8000000 INFO @ Sat, 24 Aug 2019 22:16:20: 9000000 INFO @ Sat, 24 Aug 2019 22:16:27: 10000000 INFO @ Sat, 24 Aug 2019 22:16:35: 11000000 INFO @ Sat, 24 Aug 2019 22:16:39: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 22:16:39: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 22:16:39: #1 total tags in treatment: 11596508 INFO @ Sat, 24 Aug 2019 22:16:39: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 22:16:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 22:16:39: #1 tags after filtering in treatment: 11596508 INFO @ Sat, 24 Aug 2019 22:16:39: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 22:16:39: #1 finished! INFO @ Sat, 24 Aug 2019 22:16:39: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 22:16:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 22:16:40: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 22:16:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 22:16:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5431196/SRX5431196.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431196/SRX5431196.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431196/SRX5431196.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431196/SRX5431196.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。