Job ID = 2641089 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 79,905,570 reads read : 79,905,570 reads written : 79,905,570 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:45 79905570 reads; of these: 79905570 (100.00%) were unpaired; of these: 5372962 (6.72%) aligned 0 times 61662956 (77.17%) aligned exactly 1 time 12869652 (16.11%) aligned >1 times 93.28% overall alignment rate Time searching: 00:13:45 Overall time: 00:13:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 63239127 / 74532608 = 0.8485 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 22:08:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5431192/SRX5431192.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5431192/SRX5431192.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5431192/SRX5431192.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5431192/SRX5431192.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 22:08:09: #1 read tag files... INFO @ Sat, 24 Aug 2019 22:08:09: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 22:08:19: 1000000 INFO @ Sat, 24 Aug 2019 22:08:28: 2000000 INFO @ Sat, 24 Aug 2019 22:08:38: 3000000 INFO @ Sat, 24 Aug 2019 22:08:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5431192/SRX5431192.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5431192/SRX5431192.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5431192/SRX5431192.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5431192/SRX5431192.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 22:08:38: #1 read tag files... INFO @ Sat, 24 Aug 2019 22:08:38: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 22:08:48: 1000000 INFO @ Sat, 24 Aug 2019 22:08:48: 4000000 INFO @ Sat, 24 Aug 2019 22:08:57: 2000000 INFO @ Sat, 24 Aug 2019 22:08:57: 5000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 22:09:06: 3000000 INFO @ Sat, 24 Aug 2019 22:09:07: 6000000 INFO @ Sat, 24 Aug 2019 22:09:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5431192/SRX5431192.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5431192/SRX5431192.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5431192/SRX5431192.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5431192/SRX5431192.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 22:09:08: #1 read tag files... INFO @ Sat, 24 Aug 2019 22:09:08: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 22:09:16: 4000000 INFO @ Sat, 24 Aug 2019 22:09:16: 1000000 INFO @ Sat, 24 Aug 2019 22:09:17: 7000000 INFO @ Sat, 24 Aug 2019 22:09:24: 2000000 INFO @ Sat, 24 Aug 2019 22:09:25: 5000000 INFO @ Sat, 24 Aug 2019 22:09:27: 8000000 INFO @ Sat, 24 Aug 2019 22:09:32: 3000000 INFO @ Sat, 24 Aug 2019 22:09:35: 6000000 INFO @ Sat, 24 Aug 2019 22:09:37: 9000000 INFO @ Sat, 24 Aug 2019 22:09:40: 4000000 INFO @ Sat, 24 Aug 2019 22:09:44: 7000000 INFO @ Sat, 24 Aug 2019 22:09:47: 10000000 INFO @ Sat, 24 Aug 2019 22:09:48: 5000000 INFO @ Sat, 24 Aug 2019 22:09:53: 8000000 INFO @ Sat, 24 Aug 2019 22:09:56: 6000000 INFO @ Sat, 24 Aug 2019 22:09:57: 11000000 INFO @ Sat, 24 Aug 2019 22:10:00: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 22:10:00: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 22:10:00: #1 total tags in treatment: 11293481 INFO @ Sat, 24 Aug 2019 22:10:00: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 22:10:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 22:10:00: #1 tags after filtering in treatment: 11293481 INFO @ Sat, 24 Aug 2019 22:10:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 22:10:00: #1 finished! INFO @ Sat, 24 Aug 2019 22:10:00: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 22:10:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 22:10:01: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 22:10:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 22:10:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5431192/SRX5431192.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431192/SRX5431192.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431192/SRX5431192.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431192/SRX5431192.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 22:10:03: 9000000 INFO @ Sat, 24 Aug 2019 22:10:04: 7000000 INFO @ Sat, 24 Aug 2019 22:10:11: 10000000 INFO @ Sat, 24 Aug 2019 22:10:12: 8000000 INFO @ Sat, 24 Aug 2019 22:10:20: 11000000 INFO @ Sat, 24 Aug 2019 22:10:20: 9000000 INFO @ Sat, 24 Aug 2019 22:10:22: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 22:10:22: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 22:10:22: #1 total tags in treatment: 11293481 INFO @ Sat, 24 Aug 2019 22:10:22: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 22:10:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 22:10:22: #1 tags after filtering in treatment: 11293481 INFO @ Sat, 24 Aug 2019 22:10:22: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 22:10:22: #1 finished! INFO @ Sat, 24 Aug 2019 22:10:22: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 22:10:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 22:10:23: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 22:10:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 22:10:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5431192/SRX5431192.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431192/SRX5431192.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431192/SRX5431192.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431192/SRX5431192.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 22:10:27: 10000000 INFO @ Sat, 24 Aug 2019 22:10:34: 11000000 INFO @ Sat, 24 Aug 2019 22:10:36: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 22:10:36: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 22:10:36: #1 total tags in treatment: 11293481 INFO @ Sat, 24 Aug 2019 22:10:36: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 22:10:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 22:10:37: #1 tags after filtering in treatment: 11293481 INFO @ Sat, 24 Aug 2019 22:10:37: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 22:10:37: #1 finished! INFO @ Sat, 24 Aug 2019 22:10:37: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 22:10:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 22:10:37: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 22:10:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 22:10:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5431192/SRX5431192.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431192/SRX5431192.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431192/SRX5431192.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5431192/SRX5431192.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。