Job ID = 2011936 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 36,684,748 reads read : 36,684,748 reads written : 36,684,748 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1284649.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:59 36684748 reads; of these: 36684748 (100.00%) were unpaired; of these: 3393996 (9.25%) aligned 0 times 25138343 (68.53%) aligned exactly 1 time 8152409 (22.22%) aligned >1 times 90.75% overall alignment rate Time searching: 00:06:59 Overall time: 00:06:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 21518019 / 33290752 = 0.6464 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 03:42:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX541162/SRX541162.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX541162/SRX541162.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX541162/SRX541162.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX541162/SRX541162.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 03:42:44: #1 read tag files... INFO @ Sat, 06 Jul 2019 03:42:44: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 03:42:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX541162/SRX541162.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX541162/SRX541162.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX541162/SRX541162.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX541162/SRX541162.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 03:42:44: #1 read tag files... INFO @ Sat, 06 Jul 2019 03:42:44: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 03:42:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX541162/SRX541162.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX541162/SRX541162.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX541162/SRX541162.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX541162/SRX541162.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 03:42:45: #1 read tag files... INFO @ Sat, 06 Jul 2019 03:42:45: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 03:42:52: 1000000 INFO @ Sat, 06 Jul 2019 03:42:56: 1000000 INFO @ Sat, 06 Jul 2019 03:42:57: 1000000 INFO @ Sat, 06 Jul 2019 03:43:00: 2000000 INFO @ Sat, 06 Jul 2019 03:43:06: 2000000 INFO @ Sat, 06 Jul 2019 03:43:07: 2000000 INFO @ Sat, 06 Jul 2019 03:43:08: 3000000 INFO @ Sat, 06 Jul 2019 03:43:17: 3000000 INFO @ Sat, 06 Jul 2019 03:43:18: 4000000 INFO @ Sat, 06 Jul 2019 03:43:18: 3000000 INFO @ Sat, 06 Jul 2019 03:43:26: 5000000 INFO @ Sat, 06 Jul 2019 03:43:27: 4000000 INFO @ Sat, 06 Jul 2019 03:43:27: 4000000 INFO @ Sat, 06 Jul 2019 03:43:36: 6000000 INFO @ Sat, 06 Jul 2019 03:43:37: 5000000 INFO @ Sat, 06 Jul 2019 03:43:38: 5000000 INFO @ Sat, 06 Jul 2019 03:43:45: 7000000 INFO @ Sat, 06 Jul 2019 03:43:48: 6000000 INFO @ Sat, 06 Jul 2019 03:43:48: 6000000 INFO @ Sat, 06 Jul 2019 03:43:54: 8000000 INFO @ Sat, 06 Jul 2019 03:43:58: 7000000 INFO @ Sat, 06 Jul 2019 03:43:59: 7000000 INFO @ Sat, 06 Jul 2019 03:44:04: 9000000 INFO @ Sat, 06 Jul 2019 03:44:08: 8000000 INFO @ Sat, 06 Jul 2019 03:44:09: 8000000 INFO @ Sat, 06 Jul 2019 03:44:13: 10000000 INFO @ Sat, 06 Jul 2019 03:44:19: 9000000 INFO @ Sat, 06 Jul 2019 03:44:20: 9000000 INFO @ Sat, 06 Jul 2019 03:44:22: 11000000 INFO @ Sat, 06 Jul 2019 03:44:29: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 03:44:29: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 03:44:29: #1 total tags in treatment: 11772733 INFO @ Sat, 06 Jul 2019 03:44:29: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 03:44:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 03:44:29: 10000000 INFO @ Sat, 06 Jul 2019 03:44:29: #1 tags after filtering in treatment: 11772733 INFO @ Sat, 06 Jul 2019 03:44:29: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 03:44:29: #1 finished! INFO @ Sat, 06 Jul 2019 03:44:29: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 03:44:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 03:44:30: 10000000 INFO @ Sat, 06 Jul 2019 03:44:30: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 03:44:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 03:44:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX541162/SRX541162.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 5 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX541162/SRX541162.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX541162/SRX541162.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX541162/SRX541162.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 06 Jul 2019 03:44:39: 11000000 INFO @ Sat, 06 Jul 2019 03:44:40: 11000000 INFO @ Sat, 06 Jul 2019 03:44:47: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 03:44:47: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 03:44:47: #1 total tags in treatment: 11772733 INFO @ Sat, 06 Jul 2019 03:44:47: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 03:44:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 03:44:47: #1 tags after filtering in treatment: 11772733 INFO @ Sat, 06 Jul 2019 03:44:47: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 03:44:47: #1 finished! INFO @ Sat, 06 Jul 2019 03:44:47: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 03:44:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 03:44:47: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 03:44:47: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 03:44:47: #1 total tags in treatment: 11772733 INFO @ Sat, 06 Jul 2019 03:44:47: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 03:44:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 03:44:48: #1 tags after filtering in treatment: 11772733 INFO @ Sat, 06 Jul 2019 03:44:48: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 03:44:48: #1 finished! INFO @ Sat, 06 Jul 2019 03:44:48: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 03:44:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 03:44:48: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 03:44:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 03:44:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX541162/SRX541162.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX541162/SRX541162.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX541162/SRX541162.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX541162/SRX541162.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 03:44:49: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 03:44:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 03:44:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX541162/SRX541162.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX541162/SRX541162.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX541162/SRX541162.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX541162/SRX541162.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。