Job ID = 2011923 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 3,044,447 reads read : 6,088,894 reads written : 6,088,894 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:07 3044447 reads; of these: 3044447 (100.00%) were paired; of these: 250995 (8.24%) aligned concordantly 0 times 2350457 (77.20%) aligned concordantly exactly 1 time 442995 (14.55%) aligned concordantly >1 times ---- 250995 pairs aligned concordantly 0 times; of these: 119744 (47.71%) aligned discordantly 1 time ---- 131251 pairs aligned 0 times concordantly or discordantly; of these: 262502 mates make up the pairs; of these: 178528 (68.01%) aligned 0 times 37920 (14.45%) aligned exactly 1 time 46054 (17.54%) aligned >1 times 97.07% overall alignment rate Time searching: 00:02:07 Overall time: 00:02:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 115709 / 2911404 = 0.0397 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 03:25:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5383696/SRX5383696.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5383696/SRX5383696.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5383696/SRX5383696.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5383696/SRX5383696.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 03:25:05: #1 read tag files... INFO @ Sat, 06 Jul 2019 03:25:05: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 03:25:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5383696/SRX5383696.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5383696/SRX5383696.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5383696/SRX5383696.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5383696/SRX5383696.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 03:25:06: #1 read tag files... INFO @ Sat, 06 Jul 2019 03:25:06: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 03:25:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5383696/SRX5383696.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5383696/SRX5383696.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5383696/SRX5383696.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5383696/SRX5383696.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 03:25:07: #1 read tag files... INFO @ Sat, 06 Jul 2019 03:25:07: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 03:25:11: 1000000 INFO @ Sat, 06 Jul 2019 03:25:12: 1000000 INFO @ Sat, 06 Jul 2019 03:25:14: 1000000 INFO @ Sat, 06 Jul 2019 03:25:17: 2000000 INFO @ Sat, 06 Jul 2019 03:25:19: 2000000 INFO @ Sat, 06 Jul 2019 03:25:20: 2000000 INFO @ Sat, 06 Jul 2019 03:25:23: 3000000 INFO @ Sat, 06 Jul 2019 03:25:25: 3000000 INFO @ Sat, 06 Jul 2019 03:25:27: 3000000 INFO @ Sat, 06 Jul 2019 03:25:28: 4000000 INFO @ Sat, 06 Jul 2019 03:25:31: 4000000 INFO @ Sat, 06 Jul 2019 03:25:33: 4000000 INFO @ Sat, 06 Jul 2019 03:25:34: 5000000 INFO @ Sat, 06 Jul 2019 03:25:38: 5000000 INFO @ Sat, 06 Jul 2019 03:25:38: #1 tag size is determined as 37 bps INFO @ Sat, 06 Jul 2019 03:25:38: #1 tag size = 37 INFO @ Sat, 06 Jul 2019 03:25:38: #1 total tags in treatment: 2680779 INFO @ Sat, 06 Jul 2019 03:25:38: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 03:25:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 03:25:38: #1 tags after filtering in treatment: 2300061 INFO @ Sat, 06 Jul 2019 03:25:38: #1 Redundant rate of treatment: 0.14 INFO @ Sat, 06 Jul 2019 03:25:38: #1 finished! INFO @ Sat, 06 Jul 2019 03:25:38: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 03:25:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 03:25:38: #2 number of paired peaks: 43 WARNING @ Sat, 06 Jul 2019 03:25:38: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 03:25:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5383696/SRX5383696.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5383696/SRX5383696.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5383696/SRX5383696.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5383696/SRX5383696.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 03:25:40: 5000000 INFO @ Sat, 06 Jul 2019 03:25:43: #1 tag size is determined as 37 bps INFO @ Sat, 06 Jul 2019 03:25:43: #1 tag size = 37 INFO @ Sat, 06 Jul 2019 03:25:43: #1 total tags in treatment: 2680779 INFO @ Sat, 06 Jul 2019 03:25:43: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 03:25:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 03:25:43: #1 tags after filtering in treatment: 2300061 INFO @ Sat, 06 Jul 2019 03:25:43: #1 Redundant rate of treatment: 0.14 INFO @ Sat, 06 Jul 2019 03:25:43: #1 finished! INFO @ Sat, 06 Jul 2019 03:25:43: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 03:25:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 03:25:43: #2 number of paired peaks: 43 WARNING @ Sat, 06 Jul 2019 03:25:43: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 03:25:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5383696/SRX5383696.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5383696/SRX5383696.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5383696/SRX5383696.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5383696/SRX5383696.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 03:25:44: #1 tag size is determined as 37 bps INFO @ Sat, 06 Jul 2019 03:25:44: #1 tag size = 37 INFO @ Sat, 06 Jul 2019 03:25:44: #1 total tags in treatment: 2680779 INFO @ Sat, 06 Jul 2019 03:25:44: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 03:25:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 03:25:44: #1 tags after filtering in treatment: 2300061 INFO @ Sat, 06 Jul 2019 03:25:44: #1 Redundant rate of treatment: 0.14 INFO @ Sat, 06 Jul 2019 03:25:44: #1 finished! INFO @ Sat, 06 Jul 2019 03:25:44: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 03:25:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 03:25:44: #2 number of paired peaks: 43 WARNING @ Sat, 06 Jul 2019 03:25:44: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 03:25:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5383696/SRX5383696.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5383696/SRX5383696.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5383696/SRX5383696.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5383696/SRX5383696.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。