Job ID = 2641084 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 23,958,389 reads read : 47,916,778 reads written : 23,958,389 reads 0-length : 23,958,389 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:19 23958389 reads; of these: 23958389 (100.00%) were unpaired; of these: 731859 (3.05%) aligned 0 times 21195203 (88.47%) aligned exactly 1 time 2031327 (8.48%) aligned >1 times 96.95% overall alignment rate Time searching: 00:04:19 Overall time: 00:04:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 11463086 / 23226530 = 0.4935 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 21:28:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257278/SRX5257278.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257278/SRX5257278.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257278/SRX5257278.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257278/SRX5257278.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:28:35: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:28:35: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:28:42: 1000000 INFO @ Sat, 24 Aug 2019 21:28:50: 2000000 INFO @ Sat, 24 Aug 2019 21:28:57: 3000000 INFO @ Sat, 24 Aug 2019 21:29:04: 4000000 INFO @ Sat, 24 Aug 2019 21:29:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257278/SRX5257278.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257278/SRX5257278.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257278/SRX5257278.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257278/SRX5257278.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:29:05: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:29:05: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:29:11: 5000000 INFO @ Sat, 24 Aug 2019 21:29:12: 1000000 INFO @ Sat, 24 Aug 2019 21:29:18: 6000000 INFO @ Sat, 24 Aug 2019 21:29:18: 2000000 INFO @ Sat, 24 Aug 2019 21:29:25: 3000000 INFO @ Sat, 24 Aug 2019 21:29:25: 7000000 INFO @ Sat, 24 Aug 2019 21:29:31: 4000000 INFO @ Sat, 24 Aug 2019 21:29:32: 8000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 21:29:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257278/SRX5257278.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257278/SRX5257278.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257278/SRX5257278.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257278/SRX5257278.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:29:35: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:29:35: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:29:38: 5000000 INFO @ Sat, 24 Aug 2019 21:29:39: 9000000 INFO @ Sat, 24 Aug 2019 21:29:43: 1000000 INFO @ Sat, 24 Aug 2019 21:29:44: 6000000 INFO @ Sat, 24 Aug 2019 21:29:46: 10000000 INFO @ Sat, 24 Aug 2019 21:29:50: 2000000 INFO @ Sat, 24 Aug 2019 21:29:51: 7000000 INFO @ Sat, 24 Aug 2019 21:29:54: 11000000 INFO @ Sat, 24 Aug 2019 21:29:57: 3000000 INFO @ Sat, 24 Aug 2019 21:29:57: 8000000 INFO @ Sat, 24 Aug 2019 21:29:59: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:29:59: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:29:59: #1 total tags in treatment: 11763444 INFO @ Sat, 24 Aug 2019 21:29:59: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:29:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:29:59: #1 tags after filtering in treatment: 11763444 INFO @ Sat, 24 Aug 2019 21:29:59: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:29:59: #1 finished! INFO @ Sat, 24 Aug 2019 21:29:59: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:29:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:30:00: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:30:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:30:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257278/SRX5257278.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257278/SRX5257278.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257278/SRX5257278.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257278/SRX5257278.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 21:30:04: 9000000 INFO @ Sat, 24 Aug 2019 21:30:04: 4000000 INFO @ Sat, 24 Aug 2019 21:30:10: 10000000 INFO @ Sat, 24 Aug 2019 21:30:11: 5000000 INFO @ Sat, 24 Aug 2019 21:30:16: 11000000 INFO @ Sat, 24 Aug 2019 21:30:18: 6000000 INFO @ Sat, 24 Aug 2019 21:30:21: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:30:21: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:30:21: #1 total tags in treatment: 11763444 INFO @ Sat, 24 Aug 2019 21:30:21: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:30:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:30:22: #1 tags after filtering in treatment: 11763444 INFO @ Sat, 24 Aug 2019 21:30:22: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:30:22: #1 finished! INFO @ Sat, 24 Aug 2019 21:30:22: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:30:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:30:23: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:30:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:30:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257278/SRX5257278.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257278/SRX5257278.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257278/SRX5257278.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257278/SRX5257278.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 21:30:25: 7000000 INFO @ Sat, 24 Aug 2019 21:30:32: 8000000 INFO @ Sat, 24 Aug 2019 21:30:39: 9000000 INFO @ Sat, 24 Aug 2019 21:30:46: 10000000 INFO @ Sat, 24 Aug 2019 21:30:52: 11000000 INFO @ Sat, 24 Aug 2019 21:30:58: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:30:58: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:30:58: #1 total tags in treatment: 11763444 INFO @ Sat, 24 Aug 2019 21:30:58: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:30:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:30:58: #1 tags after filtering in treatment: 11763444 INFO @ Sat, 24 Aug 2019 21:30:58: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:30:58: #1 finished! INFO @ Sat, 24 Aug 2019 21:30:58: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:30:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:30:59: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:30:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:30:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257278/SRX5257278.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257278/SRX5257278.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257278/SRX5257278.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257278/SRX5257278.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。