Job ID = 2641081 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 26,118,389 reads read : 52,236,778 reads written : 26,118,389 reads 0-length : 26,118,389 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:33 26118389 reads; of these: 26118389 (100.00%) were unpaired; of these: 664926 (2.55%) aligned 0 times 22789981 (87.26%) aligned exactly 1 time 2663482 (10.20%) aligned >1 times 97.45% overall alignment rate Time searching: 00:04:33 Overall time: 00:04:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 14531551 / 25453463 = 0.5709 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 21:28:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257275/SRX5257275.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257275/SRX5257275.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257275/SRX5257275.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257275/SRX5257275.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:28:47: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:28:47: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:28:57: 1000000 INFO @ Sat, 24 Aug 2019 21:29:06: 2000000 INFO @ Sat, 24 Aug 2019 21:29:15: 3000000 INFO @ Sat, 24 Aug 2019 21:29:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257275/SRX5257275.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257275/SRX5257275.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257275/SRX5257275.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257275/SRX5257275.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:29:17: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:29:17: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:29:25: 4000000 INFO @ Sat, 24 Aug 2019 21:29:28: 1000000 INFO @ Sat, 24 Aug 2019 21:29:35: 5000000 INFO @ Sat, 24 Aug 2019 21:29:38: 2000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 21:29:45: 6000000 INFO @ Sat, 24 Aug 2019 21:29:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257275/SRX5257275.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257275/SRX5257275.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257275/SRX5257275.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257275/SRX5257275.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:29:47: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:29:47: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:29:49: 3000000 INFO @ Sat, 24 Aug 2019 21:29:57: 7000000 INFO @ Sat, 24 Aug 2019 21:29:59: 1000000 INFO @ Sat, 24 Aug 2019 21:30:01: 4000000 INFO @ Sat, 24 Aug 2019 21:30:07: 8000000 INFO @ Sat, 24 Aug 2019 21:30:12: 2000000 INFO @ Sat, 24 Aug 2019 21:30:12: 5000000 INFO @ Sat, 24 Aug 2019 21:30:18: 9000000 INFO @ Sat, 24 Aug 2019 21:30:24: 6000000 INFO @ Sat, 24 Aug 2019 21:30:24: 3000000 INFO @ Sat, 24 Aug 2019 21:30:29: 10000000 INFO @ Sat, 24 Aug 2019 21:30:35: 7000000 INFO @ Sat, 24 Aug 2019 21:30:36: 4000000 INFO @ Sat, 24 Aug 2019 21:30:39: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:30:39: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:30:39: #1 total tags in treatment: 10921912 INFO @ Sat, 24 Aug 2019 21:30:39: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:30:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:30:39: #1 tags after filtering in treatment: 10921912 INFO @ Sat, 24 Aug 2019 21:30:39: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:30:39: #1 finished! INFO @ Sat, 24 Aug 2019 21:30:39: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:30:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:30:40: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:30:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:30:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257275/SRX5257275.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257275/SRX5257275.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257275/SRX5257275.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257275/SRX5257275.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 21:30:46: 8000000 INFO @ Sat, 24 Aug 2019 21:30:47: 5000000 INFO @ Sat, 24 Aug 2019 21:30:56: 6000000 INFO @ Sat, 24 Aug 2019 21:30:57: 9000000 INFO @ Sat, 24 Aug 2019 21:31:06: 7000000 INFO @ Sat, 24 Aug 2019 21:31:07: 10000000 INFO @ Sat, 24 Aug 2019 21:31:16: 8000000 INFO @ Sat, 24 Aug 2019 21:31:16: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:31:16: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:31:16: #1 total tags in treatment: 10921912 INFO @ Sat, 24 Aug 2019 21:31:16: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:31:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:31:17: #1 tags after filtering in treatment: 10921912 INFO @ Sat, 24 Aug 2019 21:31:17: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:31:17: #1 finished! INFO @ Sat, 24 Aug 2019 21:31:17: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:31:17: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:31:17: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:31:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:31:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257275/SRX5257275.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257275/SRX5257275.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257275/SRX5257275.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257275/SRX5257275.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 24 Aug 2019 21:31:25: 9000000 INFO @ Sat, 24 Aug 2019 21:31:34: 10000000 BigWig に変換しました。 INFO @ Sat, 24 Aug 2019 21:31:42: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:31:42: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:31:42: #1 total tags in treatment: 10921912 INFO @ Sat, 24 Aug 2019 21:31:42: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:31:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:31:42: #1 tags after filtering in treatment: 10921912 INFO @ Sat, 24 Aug 2019 21:31:42: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:31:42: #1 finished! INFO @ Sat, 24 Aug 2019 21:31:42: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:31:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:31:43: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:31:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:31:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257275/SRX5257275.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257275/SRX5257275.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257275/SRX5257275.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257275/SRX5257275.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling