Job ID = 2641070 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 16,256,681 reads read : 32,513,362 reads written : 16,256,681 reads 0-length : 16,256,681 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:48 16256681 reads; of these: 16256681 (100.00%) were unpaired; of these: 327429 (2.01%) aligned 0 times 14211785 (87.42%) aligned exactly 1 time 1717467 (10.56%) aligned >1 times 97.99% overall alignment rate Time searching: 00:02:48 Overall time: 00:02:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 8066577 / 15929252 = 0.5064 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 21:17:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257264/SRX5257264.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257264/SRX5257264.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257264/SRX5257264.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257264/SRX5257264.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:17:22: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:17:22: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:17:29: 1000000 INFO @ Sat, 24 Aug 2019 21:17:37: 2000000 INFO @ Sat, 24 Aug 2019 21:17:45: 3000000 INFO @ Sat, 24 Aug 2019 21:17:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257264/SRX5257264.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257264/SRX5257264.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257264/SRX5257264.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257264/SRX5257264.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:17:52: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:17:52: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:17:52: 4000000 INFO @ Sat, 24 Aug 2019 21:17:59: 1000000 INFO @ Sat, 24 Aug 2019 21:18:00: 5000000 INFO @ Sat, 24 Aug 2019 21:18:07: 2000000 INFO @ Sat, 24 Aug 2019 21:18:07: 6000000 INFO @ Sat, 24 Aug 2019 21:18:15: 3000000 INFO @ Sat, 24 Aug 2019 21:18:15: 7000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 21:18:21: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:18:21: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:18:21: #1 total tags in treatment: 7862675 INFO @ Sat, 24 Aug 2019 21:18:21: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:18:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:18:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257264/SRX5257264.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257264/SRX5257264.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257264/SRX5257264.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257264/SRX5257264.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:18:22: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:18:22: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:18:22: #1 tags after filtering in treatment: 7862675 INFO @ Sat, 24 Aug 2019 21:18:22: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:18:22: #1 finished! INFO @ Sat, 24 Aug 2019 21:18:22: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:18:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:18:22: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:18:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:18:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257264/SRX5257264.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257264/SRX5257264.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257264/SRX5257264.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257264/SRX5257264.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 21:18:22: 4000000 INFO @ Sat, 24 Aug 2019 21:18:29: 1000000 INFO @ Sat, 24 Aug 2019 21:18:30: 5000000 INFO @ Sat, 24 Aug 2019 21:18:37: 6000000 INFO @ Sat, 24 Aug 2019 21:18:37: 2000000 INFO @ Sat, 24 Aug 2019 21:18:45: 7000000 INFO @ Sat, 24 Aug 2019 21:18:45: 3000000 INFO @ Sat, 24 Aug 2019 21:18:51: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:18:51: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:18:51: #1 total tags in treatment: 7862675 INFO @ Sat, 24 Aug 2019 21:18:51: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:18:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:18:51: #1 tags after filtering in treatment: 7862675 INFO @ Sat, 24 Aug 2019 21:18:51: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:18:51: #1 finished! INFO @ Sat, 24 Aug 2019 21:18:51: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:18:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:18:52: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:18:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:18:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257264/SRX5257264.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257264/SRX5257264.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257264/SRX5257264.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257264/SRX5257264.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 21:18:52: 4000000 INFO @ Sat, 24 Aug 2019 21:19:00: 5000000 INFO @ Sat, 24 Aug 2019 21:19:07: 6000000 INFO @ Sat, 24 Aug 2019 21:19:14: 7000000 INFO @ Sat, 24 Aug 2019 21:19:20: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:19:20: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:19:20: #1 total tags in treatment: 7862675 INFO @ Sat, 24 Aug 2019 21:19:20: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:19:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:19:20: #1 tags after filtering in treatment: 7862675 INFO @ Sat, 24 Aug 2019 21:19:20: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:19:20: #1 finished! INFO @ Sat, 24 Aug 2019 21:19:20: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:19:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:19:21: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:19:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:19:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257264/SRX5257264.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257264/SRX5257264.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257264/SRX5257264.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257264/SRX5257264.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。