Job ID = 2641064 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 23,454,497 reads read : 46,908,994 reads written : 23,454,497 reads 0-length : 23,454,497 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:02 23454497 reads; of these: 23454497 (100.00%) were unpaired; of these: 1356662 (5.78%) aligned 0 times 19665097 (83.84%) aligned exactly 1 time 2432738 (10.37%) aligned >1 times 94.22% overall alignment rate Time searching: 00:04:03 Overall time: 00:04:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 11090847 / 22097835 = 0.5019 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 21:17:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257258/SRX5257258.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257258/SRX5257258.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257258/SRX5257258.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257258/SRX5257258.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:17:22: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:17:22: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:17:29: 1000000 INFO @ Sat, 24 Aug 2019 21:17:36: 2000000 INFO @ Sat, 24 Aug 2019 21:17:43: 3000000 INFO @ Sat, 24 Aug 2019 21:17:49: 4000000 INFO @ Sat, 24 Aug 2019 21:17:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257258/SRX5257258.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257258/SRX5257258.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257258/SRX5257258.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257258/SRX5257258.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:17:52: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:17:52: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:17:56: 5000000 INFO @ Sat, 24 Aug 2019 21:17:59: 1000000 INFO @ Sat, 24 Aug 2019 21:18:03: 6000000 INFO @ Sat, 24 Aug 2019 21:18:06: 2000000 INFO @ Sat, 24 Aug 2019 21:18:10: 7000000 INFO @ Sat, 24 Aug 2019 21:18:13: 3000000 INFO @ Sat, 24 Aug 2019 21:18:17: 8000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 21:18:20: 4000000 INFO @ Sat, 24 Aug 2019 21:18:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257258/SRX5257258.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257258/SRX5257258.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257258/SRX5257258.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257258/SRX5257258.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:18:22: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:18:22: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:18:24: 9000000 INFO @ Sat, 24 Aug 2019 21:18:27: 5000000 INFO @ Sat, 24 Aug 2019 21:18:31: 1000000 INFO @ Sat, 24 Aug 2019 21:18:31: 10000000 INFO @ Sat, 24 Aug 2019 21:18:34: 6000000 INFO @ Sat, 24 Aug 2019 21:18:38: 11000000 INFO @ Sat, 24 Aug 2019 21:18:38: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:18:38: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:18:38: #1 total tags in treatment: 11006988 INFO @ Sat, 24 Aug 2019 21:18:38: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:18:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:18:38: #1 tags after filtering in treatment: 11006988 INFO @ Sat, 24 Aug 2019 21:18:38: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:18:38: #1 finished! INFO @ Sat, 24 Aug 2019 21:18:38: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:18:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:18:39: 2000000 INFO @ Sat, 24 Aug 2019 21:18:39: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:18:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:18:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257258/SRX5257258.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257258/SRX5257258.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257258/SRX5257258.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257258/SRX5257258.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 21:18:42: 7000000 INFO @ Sat, 24 Aug 2019 21:18:47: 3000000 INFO @ Sat, 24 Aug 2019 21:18:49: 8000000 INFO @ Sat, 24 Aug 2019 21:18:55: 4000000 INFO @ Sat, 24 Aug 2019 21:18:56: 9000000 INFO @ Sat, 24 Aug 2019 21:19:03: 10000000 INFO @ Sat, 24 Aug 2019 21:19:03: 5000000 INFO @ Sat, 24 Aug 2019 21:19:10: 11000000 INFO @ Sat, 24 Aug 2019 21:19:10: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:19:10: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:19:10: #1 total tags in treatment: 11006988 INFO @ Sat, 24 Aug 2019 21:19:10: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:19:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:19:10: #1 tags after filtering in treatment: 11006988 INFO @ Sat, 24 Aug 2019 21:19:10: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:19:10: #1 finished! INFO @ Sat, 24 Aug 2019 21:19:10: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:19:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:19:11: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:19:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:19:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257258/SRX5257258.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257258/SRX5257258.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257258/SRX5257258.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257258/SRX5257258.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 21:19:11: 6000000 INFO @ Sat, 24 Aug 2019 21:19:19: 7000000 INFO @ Sat, 24 Aug 2019 21:19:27: 8000000 INFO @ Sat, 24 Aug 2019 21:19:35: 9000000 INFO @ Sat, 24 Aug 2019 21:19:43: 10000000 INFO @ Sat, 24 Aug 2019 21:19:51: 11000000 INFO @ Sat, 24 Aug 2019 21:19:51: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:19:51: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:19:51: #1 total tags in treatment: 11006988 INFO @ Sat, 24 Aug 2019 21:19:51: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:19:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:19:51: #1 tags after filtering in treatment: 11006988 INFO @ Sat, 24 Aug 2019 21:19:51: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:19:51: #1 finished! INFO @ Sat, 24 Aug 2019 21:19:51: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:19:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:19:52: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:19:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:19:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257258/SRX5257258.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257258/SRX5257258.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257258/SRX5257258.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257258/SRX5257258.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。