Job ID = 2641057 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 23,933,229 reads read : 47,866,458 reads written : 23,933,229 reads 0-length : 23,933,229 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:07 23933229 reads; of these: 23933229 (100.00%) were unpaired; of these: 2567673 (10.73%) aligned 0 times 19984033 (83.50%) aligned exactly 1 time 1381523 (5.77%) aligned >1 times 89.27% overall alignment rate Time searching: 00:04:07 Overall time: 00:04:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 14503931 / 21365556 = 0.6788 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 21:16:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257252/SRX5257252.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257252/SRX5257252.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257252/SRX5257252.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257252/SRX5257252.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:16:15: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:16:15: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:16:23: 1000000 INFO @ Sat, 24 Aug 2019 21:16:30: 2000000 INFO @ Sat, 24 Aug 2019 21:16:37: 3000000 INFO @ Sat, 24 Aug 2019 21:16:44: 4000000 INFO @ Sat, 24 Aug 2019 21:16:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257252/SRX5257252.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257252/SRX5257252.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257252/SRX5257252.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257252/SRX5257252.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:16:45: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:16:45: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:16:51: 5000000 INFO @ Sat, 24 Aug 2019 21:16:53: 1000000 INFO @ Sat, 24 Aug 2019 21:16:58: 6000000 INFO @ Sat, 24 Aug 2019 21:17:02: 2000000 INFO @ Sat, 24 Aug 2019 21:17:04: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:17:04: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:17:04: #1 total tags in treatment: 6861625 INFO @ Sat, 24 Aug 2019 21:17:04: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:17:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:17:04: #1 tags after filtering in treatment: 6861625 INFO @ Sat, 24 Aug 2019 21:17:04: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:17:04: #1 finished! INFO @ Sat, 24 Aug 2019 21:17:04: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:17:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:17:04: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:17:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:17:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257252/SRX5257252.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257252/SRX5257252.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257252/SRX5257252.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257252/SRX5257252.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 21:17:10: 3000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 21:17:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257252/SRX5257252.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257252/SRX5257252.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257252/SRX5257252.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257252/SRX5257252.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:17:15: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:17:15: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:17:17: 4000000 INFO @ Sat, 24 Aug 2019 21:17:22: 1000000 INFO @ Sat, 24 Aug 2019 21:17:25: 5000000 INFO @ Sat, 24 Aug 2019 21:17:29: 2000000 INFO @ Sat, 24 Aug 2019 21:17:33: 6000000 INFO @ Sat, 24 Aug 2019 21:17:35: 3000000 INFO @ Sat, 24 Aug 2019 21:17:40: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:17:40: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:17:40: #1 total tags in treatment: 6861625 INFO @ Sat, 24 Aug 2019 21:17:40: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:17:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:17:40: #1 tags after filtering in treatment: 6861625 INFO @ Sat, 24 Aug 2019 21:17:40: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:17:40: #1 finished! INFO @ Sat, 24 Aug 2019 21:17:40: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:17:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:17:40: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:17:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:17:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257252/SRX5257252.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257252/SRX5257252.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257252/SRX5257252.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257252/SRX5257252.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 21:17:42: 4000000 INFO @ Sat, 24 Aug 2019 21:17:48: 5000000 INFO @ Sat, 24 Aug 2019 21:17:54: 6000000 INFO @ Sat, 24 Aug 2019 21:18:00: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:18:00: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:18:00: #1 total tags in treatment: 6861625 INFO @ Sat, 24 Aug 2019 21:18:00: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:18:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:18:00: #1 tags after filtering in treatment: 6861625 INFO @ Sat, 24 Aug 2019 21:18:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:18:00: #1 finished! INFO @ Sat, 24 Aug 2019 21:18:00: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:18:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:18:00: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:18:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:18:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257252/SRX5257252.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257252/SRX5257252.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257252/SRX5257252.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257252/SRX5257252.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。