Job ID = 2641055 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 20,133,575 reads read : 40,267,150 reads written : 20,133,575 reads 0-length : 20,133,575 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:19 20133575 reads; of these: 20133575 (100.00%) were unpaired; of these: 1672415 (8.31%) aligned 0 times 17298394 (85.92%) aligned exactly 1 time 1162766 (5.78%) aligned >1 times 91.69% overall alignment rate Time searching: 00:03:19 Overall time: 00:03:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 12313165 / 18461160 = 0.6670 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 21:13:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257250/SRX5257250.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257250/SRX5257250.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257250/SRX5257250.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257250/SRX5257250.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:13:24: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:13:24: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:13:31: 1000000 INFO @ Sat, 24 Aug 2019 21:13:38: 2000000 INFO @ Sat, 24 Aug 2019 21:13:45: 3000000 INFO @ Sat, 24 Aug 2019 21:13:52: 4000000 INFO @ Sat, 24 Aug 2019 21:13:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257250/SRX5257250.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257250/SRX5257250.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257250/SRX5257250.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257250/SRX5257250.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:13:53: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:13:53: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:13:59: 5000000 INFO @ Sat, 24 Aug 2019 21:14:02: 1000000 INFO @ Sat, 24 Aug 2019 21:14:07: 6000000 INFO @ Sat, 24 Aug 2019 21:14:08: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:14:08: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:14:08: #1 total tags in treatment: 6147995 INFO @ Sat, 24 Aug 2019 21:14:08: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:14:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:14:08: #1 tags after filtering in treatment: 6147995 INFO @ Sat, 24 Aug 2019 21:14:08: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:14:08: #1 finished! INFO @ Sat, 24 Aug 2019 21:14:08: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:14:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:14:08: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:14:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:14:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257250/SRX5257250.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257250/SRX5257250.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257250/SRX5257250.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257250/SRX5257250.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 21:14:10: 2000000 INFO @ Sat, 24 Aug 2019 21:14:19: 3000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 21:14:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257250/SRX5257250.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257250/SRX5257250.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257250/SRX5257250.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257250/SRX5257250.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:14:23: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:14:23: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:14:27: 4000000 INFO @ Sat, 24 Aug 2019 21:14:30: 1000000 INFO @ Sat, 24 Aug 2019 21:14:36: 5000000 INFO @ Sat, 24 Aug 2019 21:14:36: 2000000 INFO @ Sat, 24 Aug 2019 21:14:43: 3000000 INFO @ Sat, 24 Aug 2019 21:14:44: 6000000 INFO @ Sat, 24 Aug 2019 21:14:45: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:14:45: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:14:45: #1 total tags in treatment: 6147995 INFO @ Sat, 24 Aug 2019 21:14:45: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:14:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:14:45: #1 tags after filtering in treatment: 6147995 INFO @ Sat, 24 Aug 2019 21:14:45: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:14:45: #1 finished! INFO @ Sat, 24 Aug 2019 21:14:45: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:14:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:14:46: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:14:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:14:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257250/SRX5257250.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257250/SRX5257250.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257250/SRX5257250.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257250/SRX5257250.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 21:14:50: 4000000 INFO @ Sat, 24 Aug 2019 21:14:56: 5000000 INFO @ Sat, 24 Aug 2019 21:15:03: 6000000 INFO @ Sat, 24 Aug 2019 21:15:04: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:15:04: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:15:04: #1 total tags in treatment: 6147995 INFO @ Sat, 24 Aug 2019 21:15:04: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:15:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:15:04: #1 tags after filtering in treatment: 6147995 INFO @ Sat, 24 Aug 2019 21:15:04: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:15:04: #1 finished! INFO @ Sat, 24 Aug 2019 21:15:04: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:15:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:15:04: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:15:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:15:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257250/SRX5257250.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257250/SRX5257250.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257250/SRX5257250.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257250/SRX5257250.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。