Job ID = 2641052 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 49,927,847 reads read : 99,855,694 reads written : 49,927,847 reads 0-length : 49,927,847 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:15 49927847 reads; of these: 49927847 (100.00%) were unpaired; of these: 978277 (1.96%) aligned 0 times 44650175 (89.43%) aligned exactly 1 time 4299395 (8.61%) aligned >1 times 98.04% overall alignment rate Time searching: 00:10:15 Overall time: 00:10:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 34685722 / 48949570 = 0.7086 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 21:31:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257247/SRX5257247.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257247/SRX5257247.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257247/SRX5257247.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257247/SRX5257247.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:31:05: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:31:05: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:31:16: 1000000 INFO @ Sat, 24 Aug 2019 21:31:26: 2000000 INFO @ Sat, 24 Aug 2019 21:31:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257247/SRX5257247.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257247/SRX5257247.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257247/SRX5257247.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257247/SRX5257247.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:31:35: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:31:35: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:31:36: 3000000 INFO @ Sat, 24 Aug 2019 21:31:44: 1000000 INFO @ Sat, 24 Aug 2019 21:31:46: 4000000 INFO @ Sat, 24 Aug 2019 21:31:53: 2000000 INFO @ Sat, 24 Aug 2019 21:31:56: 5000000 INFO @ Sat, 24 Aug 2019 21:32:02: 3000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 21:32:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257247/SRX5257247.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257247/SRX5257247.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257247/SRX5257247.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257247/SRX5257247.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:32:05: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:32:05: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:32:07: 6000000 INFO @ Sat, 24 Aug 2019 21:32:11: 4000000 INFO @ Sat, 24 Aug 2019 21:32:16: 1000000 INFO @ Sat, 24 Aug 2019 21:32:17: 7000000 INFO @ Sat, 24 Aug 2019 21:32:20: 5000000 INFO @ Sat, 24 Aug 2019 21:32:27: 8000000 INFO @ Sat, 24 Aug 2019 21:32:27: 2000000 INFO @ Sat, 24 Aug 2019 21:32:29: 6000000 INFO @ Sat, 24 Aug 2019 21:32:37: 9000000 INFO @ Sat, 24 Aug 2019 21:32:38: 7000000 INFO @ Sat, 24 Aug 2019 21:32:38: 3000000 INFO @ Sat, 24 Aug 2019 21:32:46: 8000000 INFO @ Sat, 24 Aug 2019 21:32:47: 10000000 INFO @ Sat, 24 Aug 2019 21:32:49: 4000000 INFO @ Sat, 24 Aug 2019 21:32:54: 9000000 INFO @ Sat, 24 Aug 2019 21:32:57: 11000000 INFO @ Sat, 24 Aug 2019 21:32:59: 5000000 INFO @ Sat, 24 Aug 2019 21:33:03: 10000000 INFO @ Sat, 24 Aug 2019 21:33:07: 12000000 INFO @ Sat, 24 Aug 2019 21:33:10: 6000000 INFO @ Sat, 24 Aug 2019 21:33:12: 11000000 INFO @ Sat, 24 Aug 2019 21:33:17: 13000000 INFO @ Sat, 24 Aug 2019 21:33:20: 12000000 INFO @ Sat, 24 Aug 2019 21:33:21: 7000000 INFO @ Sat, 24 Aug 2019 21:33:26: 14000000 INFO @ Sat, 24 Aug 2019 21:33:29: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:33:29: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:33:29: #1 total tags in treatment: 14263848 INFO @ Sat, 24 Aug 2019 21:33:29: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:33:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:33:29: #1 tags after filtering in treatment: 14263848 INFO @ Sat, 24 Aug 2019 21:33:29: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:33:29: #1 finished! INFO @ Sat, 24 Aug 2019 21:33:29: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:33:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:33:29: 13000000 INFO @ Sat, 24 Aug 2019 21:33:30: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:33:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:33:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257247/SRX5257247.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257247/SRX5257247.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257247/SRX5257247.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257247/SRX5257247.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 21:33:32: 8000000 INFO @ Sat, 24 Aug 2019 21:33:37: 14000000 INFO @ Sat, 24 Aug 2019 21:33:40: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:33:40: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:33:40: #1 total tags in treatment: 14263848 INFO @ Sat, 24 Aug 2019 21:33:40: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:33:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:33:40: #1 tags after filtering in treatment: 14263848 INFO @ Sat, 24 Aug 2019 21:33:40: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:33:40: #1 finished! INFO @ Sat, 24 Aug 2019 21:33:40: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:33:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:33:41: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:33:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:33:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257247/SRX5257247.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257247/SRX5257247.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257247/SRX5257247.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257247/SRX5257247.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 21:33:42: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 24 Aug 2019 21:33:53: 10000000 BigWig に変換しました。 INFO @ Sat, 24 Aug 2019 21:34:03: 11000000 INFO @ Sat, 24 Aug 2019 21:34:14: 12000000 INFO @ Sat, 24 Aug 2019 21:34:24: 13000000 INFO @ Sat, 24 Aug 2019 21:34:35: 14000000 INFO @ Sat, 24 Aug 2019 21:34:37: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:34:37: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:34:37: #1 total tags in treatment: 14263848 INFO @ Sat, 24 Aug 2019 21:34:37: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:34:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:34:37: #1 tags after filtering in treatment: 14263848 INFO @ Sat, 24 Aug 2019 21:34:37: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:34:37: #1 finished! INFO @ Sat, 24 Aug 2019 21:34:37: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:34:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:34:38: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:34:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:34:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257247/SRX5257247.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257247/SRX5257247.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257247/SRX5257247.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257247/SRX5257247.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling