Job ID = 2641049 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 21,070,400 reads read : 42,140,800 reads written : 21,070,400 reads 0-length : 21,070,400 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:42 21070400 reads; of these: 21070400 (100.00%) were unpaired; of these: 378619 (1.80%) aligned 0 times 18883901 (89.62%) aligned exactly 1 time 1807880 (8.58%) aligned >1 times 98.20% overall alignment rate Time searching: 00:03:42 Overall time: 00:03:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 10881888 / 20691781 = 0.5259 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 21:10:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257245/SRX5257245.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257245/SRX5257245.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257245/SRX5257245.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257245/SRX5257245.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:10:57: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:10:57: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:11:05: 1000000 INFO @ Sat, 24 Aug 2019 21:11:12: 2000000 INFO @ Sat, 24 Aug 2019 21:11:19: 3000000 INFO @ Sat, 24 Aug 2019 21:11:26: 4000000 INFO @ Sat, 24 Aug 2019 21:11:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257245/SRX5257245.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257245/SRX5257245.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257245/SRX5257245.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257245/SRX5257245.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:11:27: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:11:27: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:11:33: 5000000 INFO @ Sat, 24 Aug 2019 21:11:34: 1000000 INFO @ Sat, 24 Aug 2019 21:11:40: 2000000 INFO @ Sat, 24 Aug 2019 21:11:40: 6000000 INFO @ Sat, 24 Aug 2019 21:11:47: 3000000 INFO @ Sat, 24 Aug 2019 21:11:48: 7000000 INFO @ Sat, 24 Aug 2019 21:11:53: 4000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 21:11:55: 8000000 INFO @ Sat, 24 Aug 2019 21:11:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257245/SRX5257245.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257245/SRX5257245.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257245/SRX5257245.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257245/SRX5257245.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:11:57: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:11:57: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:11:59: 5000000 INFO @ Sat, 24 Aug 2019 21:12:02: 9000000 INFO @ Sat, 24 Aug 2019 21:12:03: 1000000 INFO @ Sat, 24 Aug 2019 21:12:06: 6000000 INFO @ Sat, 24 Aug 2019 21:12:08: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:12:08: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:12:08: #1 total tags in treatment: 9809893 INFO @ Sat, 24 Aug 2019 21:12:08: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:12:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:12:08: #1 tags after filtering in treatment: 9809893 INFO @ Sat, 24 Aug 2019 21:12:08: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:12:08: #1 finished! INFO @ Sat, 24 Aug 2019 21:12:08: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:12:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:12:09: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:12:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:12:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257245/SRX5257245.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257245/SRX5257245.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257245/SRX5257245.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257245/SRX5257245.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 21:12:10: 2000000 INFO @ Sat, 24 Aug 2019 21:12:12: 7000000 INFO @ Sat, 24 Aug 2019 21:12:16: 3000000 INFO @ Sat, 24 Aug 2019 21:12:19: 8000000 INFO @ Sat, 24 Aug 2019 21:12:22: 4000000 INFO @ Sat, 24 Aug 2019 21:12:25: 9000000 INFO @ Sat, 24 Aug 2019 21:12:29: 5000000 INFO @ Sat, 24 Aug 2019 21:12:31: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:12:31: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:12:31: #1 total tags in treatment: 9809893 INFO @ Sat, 24 Aug 2019 21:12:31: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:12:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:12:31: #1 tags after filtering in treatment: 9809893 INFO @ Sat, 24 Aug 2019 21:12:31: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:12:31: #1 finished! INFO @ Sat, 24 Aug 2019 21:12:31: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:12:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:12:32: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:12:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:12:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257245/SRX5257245.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257245/SRX5257245.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257245/SRX5257245.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257245/SRX5257245.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 21:12:35: 6000000 INFO @ Sat, 24 Aug 2019 21:12:41: 7000000 INFO @ Sat, 24 Aug 2019 21:12:48: 8000000 INFO @ Sat, 24 Aug 2019 21:12:54: 9000000 INFO @ Sat, 24 Aug 2019 21:12:59: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:12:59: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:12:59: #1 total tags in treatment: 9809893 INFO @ Sat, 24 Aug 2019 21:12:59: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:12:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:12:59: #1 tags after filtering in treatment: 9809893 INFO @ Sat, 24 Aug 2019 21:12:59: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:12:59: #1 finished! INFO @ Sat, 24 Aug 2019 21:12:59: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:12:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:13:00: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:13:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:13:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257245/SRX5257245.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257245/SRX5257245.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257245/SRX5257245.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257245/SRX5257245.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。