Job ID = 2641047 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 33,202,790 reads read : 66,405,580 reads written : 33,202,790 reads 0-length : 33,202,790 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:43 33202790 reads; of these: 33202790 (100.00%) were unpaired; of these: 917924 (2.76%) aligned 0 times 29685258 (89.41%) aligned exactly 1 time 2599608 (7.83%) aligned >1 times 97.24% overall alignment rate Time searching: 00:05:43 Overall time: 00:05:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 18723168 / 32284866 = 0.5799 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 21:18:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257243/SRX5257243.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257243/SRX5257243.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257243/SRX5257243.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257243/SRX5257243.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:18:38: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:18:38: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:18:46: 1000000 INFO @ Sat, 24 Aug 2019 21:18:52: 2000000 INFO @ Sat, 24 Aug 2019 21:18:59: 3000000 INFO @ Sat, 24 Aug 2019 21:19:06: 4000000 INFO @ Sat, 24 Aug 2019 21:19:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257243/SRX5257243.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257243/SRX5257243.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257243/SRX5257243.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257243/SRX5257243.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:19:08: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:19:08: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:19:13: 5000000 INFO @ Sat, 24 Aug 2019 21:19:16: 1000000 INFO @ Sat, 24 Aug 2019 21:19:20: 6000000 INFO @ Sat, 24 Aug 2019 21:19:23: 2000000 INFO @ Sat, 24 Aug 2019 21:19:27: 7000000 INFO @ Sat, 24 Aug 2019 21:19:30: 3000000 INFO @ Sat, 24 Aug 2019 21:19:33: 8000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 21:19:37: 4000000 INFO @ Sat, 24 Aug 2019 21:19:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257243/SRX5257243.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257243/SRX5257243.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257243/SRX5257243.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257243/SRX5257243.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:19:38: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:19:38: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:19:41: 9000000 INFO @ Sat, 24 Aug 2019 21:19:44: 5000000 INFO @ Sat, 24 Aug 2019 21:19:46: 1000000 INFO @ Sat, 24 Aug 2019 21:19:48: 10000000 INFO @ Sat, 24 Aug 2019 21:19:50: 6000000 INFO @ Sat, 24 Aug 2019 21:19:53: 2000000 INFO @ Sat, 24 Aug 2019 21:19:55: 11000000 INFO @ Sat, 24 Aug 2019 21:19:57: 7000000 INFO @ Sat, 24 Aug 2019 21:19:59: 3000000 INFO @ Sat, 24 Aug 2019 21:20:02: 12000000 INFO @ Sat, 24 Aug 2019 21:20:04: 8000000 INFO @ Sat, 24 Aug 2019 21:20:06: 4000000 INFO @ Sat, 24 Aug 2019 21:20:09: 13000000 INFO @ Sat, 24 Aug 2019 21:20:12: 9000000 INFO @ Sat, 24 Aug 2019 21:20:13: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:20:13: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:20:13: #1 total tags in treatment: 13561698 INFO @ Sat, 24 Aug 2019 21:20:13: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:20:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:20:13: #1 tags after filtering in treatment: 13561698 INFO @ Sat, 24 Aug 2019 21:20:13: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:20:13: #1 finished! INFO @ Sat, 24 Aug 2019 21:20:13: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:20:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:20:13: 5000000 INFO @ Sat, 24 Aug 2019 21:20:14: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:20:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:20:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257243/SRX5257243.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257243/SRX5257243.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257243/SRX5257243.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257243/SRX5257243.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 21:20:19: 10000000 INFO @ Sat, 24 Aug 2019 21:20:20: 6000000 INFO @ Sat, 24 Aug 2019 21:20:26: 11000000 INFO @ Sat, 24 Aug 2019 21:20:26: 7000000 INFO @ Sat, 24 Aug 2019 21:20:33: 12000000 INFO @ Sat, 24 Aug 2019 21:20:33: 8000000 INFO @ Sat, 24 Aug 2019 21:20:39: 13000000 INFO @ Sat, 24 Aug 2019 21:20:40: 9000000 INFO @ Sat, 24 Aug 2019 21:20:43: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:20:43: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:20:43: #1 total tags in treatment: 13561698 INFO @ Sat, 24 Aug 2019 21:20:43: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:20:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:20:44: #1 tags after filtering in treatment: 13561698 INFO @ Sat, 24 Aug 2019 21:20:44: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:20:44: #1 finished! INFO @ Sat, 24 Aug 2019 21:20:44: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:20:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:20:45: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:20:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:20:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257243/SRX5257243.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257243/SRX5257243.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257243/SRX5257243.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257243/SRX5257243.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 21:20:47: 10000000 INFO @ Sat, 24 Aug 2019 21:20:54: 11000000 INFO @ Sat, 24 Aug 2019 21:21:01: 12000000 INFO @ Sat, 24 Aug 2019 21:21:08: 13000000 INFO @ Sat, 24 Aug 2019 21:21:11: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:21:11: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:21:11: #1 total tags in treatment: 13561698 INFO @ Sat, 24 Aug 2019 21:21:11: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:21:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:21:12: #1 tags after filtering in treatment: 13561698 INFO @ Sat, 24 Aug 2019 21:21:12: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:21:12: #1 finished! INFO @ Sat, 24 Aug 2019 21:21:12: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:21:12: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:21:13: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:21:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:21:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257243/SRX5257243.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257243/SRX5257243.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257243/SRX5257243.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257243/SRX5257243.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。