Job ID = 2641044 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-08-24T11:54:13 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T11:54:13 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '165.112.9.232' from '172.19.7.65' 2019-08-24T11:54:13 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'sra-download.ncbi.nlm.nih.gov' (165.112.9.232) from '172.19.7.65' 2019-08-24T11:54:13 fasterq-dump.2.9.6 err: connection failed while opening file within cryptographic module - error with https open 'https://sra-download.ncbi.nlm.nih.gov/traces/sra75/SRR/008252/SRR8450270' 2019-08-24T11:54:13 fasterq-dump.2.9.6 err: invalid accession 'SRR8450270' spots read : 19,728,723 reads read : 39,457,446 reads written : 19,728,723 reads 0-length : 19,728,723 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:47 19728723 reads; of these: 19728723 (100.00%) were unpaired; of these: 861518 (4.37%) aligned 0 times 17066823 (86.51%) aligned exactly 1 time 1800382 (9.13%) aligned >1 times 95.63% overall alignment rate Time searching: 00:03:47 Overall time: 00:03:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 10099425 / 18867205 = 0.5353 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 21:09:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257241/SRX5257241.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257241/SRX5257241.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257241/SRX5257241.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257241/SRX5257241.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:09:14: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:09:14: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:09:23: 1000000 INFO @ Sat, 24 Aug 2019 21:09:32: 2000000 INFO @ Sat, 24 Aug 2019 21:09:41: 3000000 INFO @ Sat, 24 Aug 2019 21:09:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257241/SRX5257241.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257241/SRX5257241.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257241/SRX5257241.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257241/SRX5257241.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:09:43: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:09:43: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:09:50: 4000000 INFO @ Sat, 24 Aug 2019 21:09:52: 1000000 INFO @ Sat, 24 Aug 2019 21:09:59: 5000000 INFO @ Sat, 24 Aug 2019 21:10:02: 2000000 INFO @ Sat, 24 Aug 2019 21:10:08: 6000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 21:10:12: 3000000 INFO @ Sat, 24 Aug 2019 21:10:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257241/SRX5257241.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257241/SRX5257241.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257241/SRX5257241.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257241/SRX5257241.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 21:10:13: #1 read tag files... INFO @ Sat, 24 Aug 2019 21:10:13: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 21:10:17: 7000000 INFO @ Sat, 24 Aug 2019 21:10:21: 4000000 INFO @ Sat, 24 Aug 2019 21:10:21: 1000000 INFO @ Sat, 24 Aug 2019 21:10:27: 8000000 INFO @ Sat, 24 Aug 2019 21:10:30: 2000000 INFO @ Sat, 24 Aug 2019 21:10:31: 5000000 INFO @ Sat, 24 Aug 2019 21:10:33: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:10:33: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:10:33: #1 total tags in treatment: 8767780 INFO @ Sat, 24 Aug 2019 21:10:33: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:10:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:10:34: #1 tags after filtering in treatment: 8767780 INFO @ Sat, 24 Aug 2019 21:10:34: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:10:34: #1 finished! INFO @ Sat, 24 Aug 2019 21:10:34: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:10:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:10:34: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:10:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:10:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257241/SRX5257241.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257241/SRX5257241.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257241/SRX5257241.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257241/SRX5257241.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 21:10:38: 3000000 INFO @ Sat, 24 Aug 2019 21:10:40: 6000000 INFO @ Sat, 24 Aug 2019 21:10:46: 4000000 INFO @ Sat, 24 Aug 2019 21:10:49: 7000000 INFO @ Sat, 24 Aug 2019 21:10:54: 5000000 INFO @ Sat, 24 Aug 2019 21:10:58: 8000000 INFO @ Sat, 24 Aug 2019 21:11:02: 6000000 INFO @ Sat, 24 Aug 2019 21:11:04: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:11:04: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:11:04: #1 total tags in treatment: 8767780 INFO @ Sat, 24 Aug 2019 21:11:04: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:11:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:11:04: #1 tags after filtering in treatment: 8767780 INFO @ Sat, 24 Aug 2019 21:11:04: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:11:04: #1 finished! INFO @ Sat, 24 Aug 2019 21:11:04: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:11:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:11:05: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:11:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:11:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257241/SRX5257241.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257241/SRX5257241.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257241/SRX5257241.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257241/SRX5257241.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 21:11:10: 7000000 INFO @ Sat, 24 Aug 2019 21:11:17: 8000000 INFO @ Sat, 24 Aug 2019 21:11:23: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:11:23: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:11:23: #1 total tags in treatment: 8767780 INFO @ Sat, 24 Aug 2019 21:11:23: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:11:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:11:23: #1 tags after filtering in treatment: 8767780 INFO @ Sat, 24 Aug 2019 21:11:23: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:11:23: #1 finished! INFO @ Sat, 24 Aug 2019 21:11:23: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:11:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:11:24: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:11:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:11:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257241/SRX5257241.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257241/SRX5257241.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257241/SRX5257241.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257241/SRX5257241.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。