Job ID = 2641030 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 23,386,281 reads read : 23,386,281 reads written : 23,386,281 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:50 23386281 reads; of these: 23386281 (100.00%) were unpaired; of these: 1199433 (5.13%) aligned 0 times 19589889 (83.77%) aligned exactly 1 time 2596959 (11.10%) aligned >1 times 94.87% overall alignment rate Time searching: 00:04:50 Overall time: 00:04:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 11631916 / 22186848 = 0.5243 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 20:58:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257227/SRX5257227.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257227/SRX5257227.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257227/SRX5257227.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257227/SRX5257227.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 20:58:02: #1 read tag files... INFO @ Sat, 24 Aug 2019 20:58:02: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 20:58:09: 1000000 INFO @ Sat, 24 Aug 2019 20:58:17: 2000000 INFO @ Sat, 24 Aug 2019 20:58:24: 3000000 INFO @ Sat, 24 Aug 2019 20:58:31: 4000000 INFO @ Sat, 24 Aug 2019 20:58:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257227/SRX5257227.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257227/SRX5257227.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257227/SRX5257227.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257227/SRX5257227.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 20:58:31: #1 read tag files... INFO @ Sat, 24 Aug 2019 20:58:31: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 20:58:38: 5000000 INFO @ Sat, 24 Aug 2019 20:58:39: 1000000 INFO @ Sat, 24 Aug 2019 20:58:45: 6000000 INFO @ Sat, 24 Aug 2019 20:58:46: 2000000 INFO @ Sat, 24 Aug 2019 20:58:52: 7000000 INFO @ Sat, 24 Aug 2019 20:58:53: 3000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 20:58:59: 8000000 INFO @ Sat, 24 Aug 2019 20:59:00: 4000000 INFO @ Sat, 24 Aug 2019 20:59:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257227/SRX5257227.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257227/SRX5257227.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257227/SRX5257227.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257227/SRX5257227.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 20:59:01: #1 read tag files... INFO @ Sat, 24 Aug 2019 20:59:01: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 20:59:06: 9000000 INFO @ Sat, 24 Aug 2019 20:59:07: 5000000 INFO @ Sat, 24 Aug 2019 20:59:10: 1000000 INFO @ Sat, 24 Aug 2019 20:59:13: 10000000 INFO @ Sat, 24 Aug 2019 20:59:14: 6000000 INFO @ Sat, 24 Aug 2019 20:59:18: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 20:59:18: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 20:59:18: #1 total tags in treatment: 10554932 INFO @ Sat, 24 Aug 2019 20:59:18: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 20:59:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 20:59:18: #1 tags after filtering in treatment: 10554932 INFO @ Sat, 24 Aug 2019 20:59:18: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 20:59:18: #1 finished! INFO @ Sat, 24 Aug 2019 20:59:18: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 20:59:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 20:59:18: 2000000 INFO @ Sat, 24 Aug 2019 20:59:19: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 20:59:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 20:59:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257227/SRX5257227.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257227/SRX5257227.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257227/SRX5257227.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257227/SRX5257227.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 20:59:21: 7000000 INFO @ Sat, 24 Aug 2019 20:59:27: 3000000 INFO @ Sat, 24 Aug 2019 20:59:28: 8000000 INFO @ Sat, 24 Aug 2019 20:59:35: 4000000 INFO @ Sat, 24 Aug 2019 20:59:35: 9000000 INFO @ Sat, 24 Aug 2019 20:59:42: 10000000 INFO @ Sat, 24 Aug 2019 20:59:43: 5000000 INFO @ Sat, 24 Aug 2019 20:59:46: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 20:59:46: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 20:59:46: #1 total tags in treatment: 10554932 INFO @ Sat, 24 Aug 2019 20:59:46: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 20:59:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 20:59:46: #1 tags after filtering in treatment: 10554932 INFO @ Sat, 24 Aug 2019 20:59:46: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 20:59:46: #1 finished! INFO @ Sat, 24 Aug 2019 20:59:46: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 20:59:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 20:59:47: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 20:59:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 20:59:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257227/SRX5257227.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257227/SRX5257227.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257227/SRX5257227.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257227/SRX5257227.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 20:59:51: 6000000 INFO @ Sat, 24 Aug 2019 20:59:59: 7000000 INFO @ Sat, 24 Aug 2019 21:00:07: 8000000 INFO @ Sat, 24 Aug 2019 21:00:15: 9000000 INFO @ Sat, 24 Aug 2019 21:00:23: 10000000 INFO @ Sat, 24 Aug 2019 21:00:28: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 21:00:28: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 21:00:28: #1 total tags in treatment: 10554932 INFO @ Sat, 24 Aug 2019 21:00:28: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 21:00:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 21:00:28: #1 tags after filtering in treatment: 10554932 INFO @ Sat, 24 Aug 2019 21:00:28: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 21:00:28: #1 finished! INFO @ Sat, 24 Aug 2019 21:00:28: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 21:00:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 21:00:29: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 21:00:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 21:00:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257227/SRX5257227.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257227/SRX5257227.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257227/SRX5257227.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257227/SRX5257227.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。