Job ID = 2641025 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 19,768,254 reads read : 19,768,254 reads written : 19,768,254 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:42 19768254 reads; of these: 19768254 (100.00%) were unpaired; of these: 1021681 (5.17%) aligned 0 times 16743248 (84.70%) aligned exactly 1 time 2003325 (10.13%) aligned >1 times 94.83% overall alignment rate Time searching: 00:03:42 Overall time: 00:03:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 8621314 / 18746573 = 0.4599 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 20:49:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257222/SRX5257222.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257222/SRX5257222.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257222/SRX5257222.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257222/SRX5257222.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 20:49:55: #1 read tag files... INFO @ Sat, 24 Aug 2019 20:49:55: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 20:50:03: 1000000 INFO @ Sat, 24 Aug 2019 20:50:10: 2000000 INFO @ Sat, 24 Aug 2019 20:50:16: 3000000 INFO @ Sat, 24 Aug 2019 20:50:23: 4000000 INFO @ Sat, 24 Aug 2019 20:50:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257222/SRX5257222.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257222/SRX5257222.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257222/SRX5257222.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257222/SRX5257222.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 20:50:24: #1 read tag files... INFO @ Sat, 24 Aug 2019 20:50:24: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 20:50:31: 5000000 INFO @ Sat, 24 Aug 2019 20:50:32: 1000000 INFO @ Sat, 24 Aug 2019 20:50:38: 6000000 INFO @ Sat, 24 Aug 2019 20:50:39: 2000000 INFO @ Sat, 24 Aug 2019 20:50:45: 7000000 INFO @ Sat, 24 Aug 2019 20:50:46: 3000000 INFO @ Sat, 24 Aug 2019 20:50:52: 8000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 20:50:53: 4000000 INFO @ Sat, 24 Aug 2019 20:50:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257222/SRX5257222.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257222/SRX5257222.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257222/SRX5257222.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257222/SRX5257222.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 20:50:54: #1 read tag files... INFO @ Sat, 24 Aug 2019 20:50:54: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 20:50:59: 9000000 INFO @ Sat, 24 Aug 2019 20:51:00: 5000000 INFO @ Sat, 24 Aug 2019 20:51:01: 1000000 INFO @ Sat, 24 Aug 2019 20:51:06: 10000000 INFO @ Sat, 24 Aug 2019 20:51:07: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 20:51:07: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 20:51:07: #1 total tags in treatment: 10125259 INFO @ Sat, 24 Aug 2019 20:51:07: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 20:51:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 20:51:07: #1 tags after filtering in treatment: 10125259 INFO @ Sat, 24 Aug 2019 20:51:07: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 20:51:07: #1 finished! INFO @ Sat, 24 Aug 2019 20:51:07: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 20:51:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 20:51:07: 6000000 INFO @ Sat, 24 Aug 2019 20:51:08: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 20:51:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 20:51:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257222/SRX5257222.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257222/SRX5257222.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257222/SRX5257222.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257222/SRX5257222.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 20:51:08: 2000000 INFO @ Sat, 24 Aug 2019 20:51:14: 7000000 INFO @ Sat, 24 Aug 2019 20:51:14: 3000000 INFO @ Sat, 24 Aug 2019 20:51:21: 4000000 INFO @ Sat, 24 Aug 2019 20:51:21: 8000000 INFO @ Sat, 24 Aug 2019 20:51:27: 5000000 INFO @ Sat, 24 Aug 2019 20:51:28: 9000000 INFO @ Sat, 24 Aug 2019 20:51:34: 6000000 INFO @ Sat, 24 Aug 2019 20:51:35: 10000000 INFO @ Sat, 24 Aug 2019 20:51:36: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 20:51:36: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 20:51:36: #1 total tags in treatment: 10125259 INFO @ Sat, 24 Aug 2019 20:51:36: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 20:51:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 20:51:36: #1 tags after filtering in treatment: 10125259 INFO @ Sat, 24 Aug 2019 20:51:36: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 20:51:36: #1 finished! INFO @ Sat, 24 Aug 2019 20:51:36: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 20:51:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 20:51:37: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 20:51:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 20:51:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257222/SRX5257222.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257222/SRX5257222.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257222/SRX5257222.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257222/SRX5257222.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 20:51:40: 7000000 INFO @ Sat, 24 Aug 2019 20:51:46: 8000000 INFO @ Sat, 24 Aug 2019 20:51:53: 9000000 INFO @ Sat, 24 Aug 2019 20:51:59: 10000000 INFO @ Sat, 24 Aug 2019 20:52:00: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 20:52:00: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 20:52:00: #1 total tags in treatment: 10125259 INFO @ Sat, 24 Aug 2019 20:52:00: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 20:52:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 20:52:00: #1 tags after filtering in treatment: 10125259 INFO @ Sat, 24 Aug 2019 20:52:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 20:52:00: #1 finished! INFO @ Sat, 24 Aug 2019 20:52:00: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 20:52:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 20:52:01: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 20:52:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 20:52:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257222/SRX5257222.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257222/SRX5257222.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257222/SRX5257222.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257222/SRX5257222.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。