Job ID = 2641023 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 27,500,960 reads read : 27,500,960 reads written : 27,500,960 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:58 27500960 reads; of these: 27500960 (100.00%) were unpaired; of these: 1552391 (5.64%) aligned 0 times 22715606 (82.60%) aligned exactly 1 time 3232963 (11.76%) aligned >1 times 94.36% overall alignment rate Time searching: 00:04:58 Overall time: 00:04:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 14857379 / 25948569 = 0.5726 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 20:54:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257220/SRX5257220.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257220/SRX5257220.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257220/SRX5257220.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257220/SRX5257220.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 20:54:51: #1 read tag files... INFO @ Sat, 24 Aug 2019 20:54:51: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 20:54:58: 1000000 INFO @ Sat, 24 Aug 2019 20:55:05: 2000000 INFO @ Sat, 24 Aug 2019 20:55:12: 3000000 INFO @ Sat, 24 Aug 2019 20:55:20: 4000000 INFO @ Sat, 24 Aug 2019 20:55:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257220/SRX5257220.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257220/SRX5257220.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257220/SRX5257220.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257220/SRX5257220.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 20:55:20: #1 read tag files... INFO @ Sat, 24 Aug 2019 20:55:20: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 20:55:27: 5000000 INFO @ Sat, 24 Aug 2019 20:55:28: 1000000 INFO @ Sat, 24 Aug 2019 20:55:34: 6000000 INFO @ Sat, 24 Aug 2019 20:55:35: 2000000 INFO @ Sat, 24 Aug 2019 20:55:41: 7000000 INFO @ Sat, 24 Aug 2019 20:55:43: 3000000 INFO @ Sat, 24 Aug 2019 20:55:48: 8000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 20:55:50: 4000000 INFO @ Sat, 24 Aug 2019 20:55:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5257220/SRX5257220.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5257220/SRX5257220.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5257220/SRX5257220.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5257220/SRX5257220.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 20:55:50: #1 read tag files... INFO @ Sat, 24 Aug 2019 20:55:50: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 20:55:55: 9000000 INFO @ Sat, 24 Aug 2019 20:55:57: 5000000 INFO @ Sat, 24 Aug 2019 20:55:58: 1000000 INFO @ Sat, 24 Aug 2019 20:56:03: 10000000 INFO @ Sat, 24 Aug 2019 20:56:04: 6000000 INFO @ Sat, 24 Aug 2019 20:56:06: 2000000 INFO @ Sat, 24 Aug 2019 20:56:10: 11000000 INFO @ Sat, 24 Aug 2019 20:56:11: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 20:56:11: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 20:56:11: #1 total tags in treatment: 11091190 INFO @ Sat, 24 Aug 2019 20:56:11: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 20:56:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 20:56:11: #1 tags after filtering in treatment: 11091190 INFO @ Sat, 24 Aug 2019 20:56:11: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 20:56:11: #1 finished! INFO @ Sat, 24 Aug 2019 20:56:11: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 20:56:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 20:56:11: 7000000 INFO @ Sat, 24 Aug 2019 20:56:12: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 20:56:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 20:56:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257220/SRX5257220.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257220/SRX5257220.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257220/SRX5257220.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257220/SRX5257220.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 20:56:13: 3000000 INFO @ Sat, 24 Aug 2019 20:56:19: 8000000 INFO @ Sat, 24 Aug 2019 20:56:20: 4000000 INFO @ Sat, 24 Aug 2019 20:56:26: 9000000 INFO @ Sat, 24 Aug 2019 20:56:27: 5000000 INFO @ Sat, 24 Aug 2019 20:56:33: 10000000 INFO @ Sat, 24 Aug 2019 20:56:35: 6000000 INFO @ Sat, 24 Aug 2019 20:56:41: 11000000 INFO @ Sat, 24 Aug 2019 20:56:41: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 20:56:41: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 20:56:41: #1 total tags in treatment: 11091190 INFO @ Sat, 24 Aug 2019 20:56:41: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 20:56:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 20:56:42: #1 tags after filtering in treatment: 11091190 INFO @ Sat, 24 Aug 2019 20:56:42: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 20:56:42: #1 finished! INFO @ Sat, 24 Aug 2019 20:56:42: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 20:56:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 20:56:42: 7000000 INFO @ Sat, 24 Aug 2019 20:56:42: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 20:56:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 20:56:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257220/SRX5257220.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257220/SRX5257220.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257220/SRX5257220.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257220/SRX5257220.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 20:56:49: 8000000 INFO @ Sat, 24 Aug 2019 20:56:56: 9000000 INFO @ Sat, 24 Aug 2019 20:57:03: 10000000 INFO @ Sat, 24 Aug 2019 20:57:11: 11000000 INFO @ Sat, 24 Aug 2019 20:57:11: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 20:57:11: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 20:57:11: #1 total tags in treatment: 11091190 INFO @ Sat, 24 Aug 2019 20:57:11: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 20:57:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 20:57:12: #1 tags after filtering in treatment: 11091190 INFO @ Sat, 24 Aug 2019 20:57:12: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 20:57:12: #1 finished! INFO @ Sat, 24 Aug 2019 20:57:12: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 20:57:12: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 20:57:12: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 20:57:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 20:57:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5257220/SRX5257220.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257220/SRX5257220.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257220/SRX5257220.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5257220/SRX5257220.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。