Job ID = 2011865 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 4,148,044 reads read : 4,148,044 reads written : 4,148,044 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR8433453.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:43 4148044 reads; of these: 4148044 (100.00%) were unpaired; of these: 478693 (11.54%) aligned 0 times 2436222 (58.73%) aligned exactly 1 time 1233129 (29.73%) aligned >1 times 88.46% overall alignment rate Time searching: 00:00:43 Overall time: 00:00:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1519856 / 3669351 = 0.4142 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 02:54:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5241172/SRX5241172.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5241172/SRX5241172.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5241172/SRX5241172.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5241172/SRX5241172.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:54:15: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:54:15: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:54:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5241172/SRX5241172.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5241172/SRX5241172.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5241172/SRX5241172.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5241172/SRX5241172.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:54:16: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:54:16: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:54:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5241172/SRX5241172.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5241172/SRX5241172.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5241172/SRX5241172.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5241172/SRX5241172.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:54:17: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:54:17: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:54:23: 1000000 INFO @ Sat, 06 Jul 2019 02:54:25: 1000000 INFO @ Sat, 06 Jul 2019 02:54:26: 1000000 INFO @ Sat, 06 Jul 2019 02:54:32: 2000000 INFO @ Sat, 06 Jul 2019 02:54:33: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:54:33: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:54:33: #1 total tags in treatment: 2149495 INFO @ Sat, 06 Jul 2019 02:54:33: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:54:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:54:33: #1 tags after filtering in treatment: 2149495 INFO @ Sat, 06 Jul 2019 02:54:33: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:54:33: #1 finished! INFO @ Sat, 06 Jul 2019 02:54:33: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:54:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:54:33: #2 number of paired peaks: 38 WARNING @ Sat, 06 Jul 2019 02:54:33: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:54:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5241172/SRX5241172.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5241172/SRX5241172.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5241172/SRX5241172.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5241172/SRX5241172.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 02:54:34: 2000000 INFO @ Sat, 06 Jul 2019 02:54:35: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:54:35: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:54:35: #1 total tags in treatment: 2149495 INFO @ Sat, 06 Jul 2019 02:54:35: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:54:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:54:35: #1 tags after filtering in treatment: 2149495 INFO @ Sat, 06 Jul 2019 02:54:35: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:54:35: #1 finished! INFO @ Sat, 06 Jul 2019 02:54:35: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:54:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:54:36: #2 number of paired peaks: 38 WARNING @ Sat, 06 Jul 2019 02:54:36: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:54:36: Process for pairing-model is terminated! INFO @ Sat, 06 Jul 2019 02:54:37: 2000000 INFO @ Sat, 06 Jul 2019 02:54:38: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:54:38: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:54:38: #1 total tags in treatment: 2149495 INFO @ Sat, 06 Jul 2019 02:54:38: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:54:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:54:38: #1 tags after filtering in treatment: 2149495 INFO @ Sat, 06 Jul 2019 02:54:38: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:54:38: #1 finished! INFO @ Sat, 06 Jul 2019 02:54:38: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:54:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:54:38: #2 number of paired peaks: 38 WARNING @ Sat, 06 Jul 2019 02:54:38: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:54:38: Process for pairing-model is terminated! BedGraph に変換しました。 BigWig に変換中... cut: /home/okishinya/chipatlas/results/sacCer3/SRX5241172/SRX5241172.10_peaks.narrowPeakcut: /home/okishinya/chipatlas/results/sacCer3/SRX5241172/SRX5241172.20_peaks.narrowPeak: No such file or directory : No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5241172/SRX5241172.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5241172/SRX5241172.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5241172/SRX5241172.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 4 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5241172/SRX5241172.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5241172/SRX5241172.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5241172/SRX5241172.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。