Job ID = 2011803 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 2,719,558 reads read : 5,439,116 reads written : 2,719,558 reads 0-length : 2,719,558 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:39 2719558 reads; of these: 2719558 (100.00%) were unpaired; of these: 781720 (28.74%) aligned 0 times 1755525 (64.55%) aligned exactly 1 time 182313 (6.70%) aligned >1 times 71.26% overall alignment rate Time searching: 00:00:39 Overall time: 00:00:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 775910 / 1937838 = 0.4004 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 02:43:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5028233/SRX5028233.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5028233/SRX5028233.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5028233/SRX5028233.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5028233/SRX5028233.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:43:30: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:43:30: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:43:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5028233/SRX5028233.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5028233/SRX5028233.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5028233/SRX5028233.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5028233/SRX5028233.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:43:31: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:43:31: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:43:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5028233/SRX5028233.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5028233/SRX5028233.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5028233/SRX5028233.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5028233/SRX5028233.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:43:32: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:43:32: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:43:40: 1000000 INFO @ Sat, 06 Jul 2019 02:43:41: 1000000 INFO @ Sat, 06 Jul 2019 02:43:42: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 02:43:42: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 02:43:42: #1 total tags in treatment: 1161928 INFO @ Sat, 06 Jul 2019 02:43:42: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:43:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:43:42: #1 tags after filtering in treatment: 1161928 INFO @ Sat, 06 Jul 2019 02:43:42: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:43:42: #1 finished! INFO @ Sat, 06 Jul 2019 02:43:42: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:43:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:43:42: #2 number of paired peaks: 1 WARNING @ Sat, 06 Jul 2019 02:43:42: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:43:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5028233/SRX5028233.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5028233/SRX5028233.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5028233/SRX5028233.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5028233/SRX5028233.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 02:43:43: 1000000 INFO @ Sat, 06 Jul 2019 02:43:43: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 02:43:43: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 02:43:43: #1 total tags in treatment: 1161928 INFO @ Sat, 06 Jul 2019 02:43:43: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:43:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:43:43: #1 tags after filtering in treatment: 1161928 INFO @ Sat, 06 Jul 2019 02:43:43: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:43:43: #1 finished! INFO @ Sat, 06 Jul 2019 02:43:43: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:43:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:43:43: #2 number of paired peaks: 1 WARNING @ Sat, 06 Jul 2019 02:43:43: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:43:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5028233/SRX5028233.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5028233/SRX5028233.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5028233/SRX5028233.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5028233/SRX5028233.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 02:43:44: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 02:43:44: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 02:43:44: #1 total tags in treatment: 1161928 INFO @ Sat, 06 Jul 2019 02:43:44: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:43:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:43:44: #1 tags after filtering in treatment: 1161928 INFO @ Sat, 06 Jul 2019 02:43:44: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:43:44: #1 finished! INFO @ Sat, 06 Jul 2019 02:43:44: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:43:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:43:44: #2 number of paired peaks: 1 WARNING @ Sat, 06 Jul 2019 02:43:44: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:43:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5028233/SRX5028233.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5028233/SRX5028233.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5028233/SRX5028233.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5028233/SRX5028233.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。