Job ID = 2011769 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-07-05T17:22:51 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T17:22:51 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '130.14.250.24' from '172.19.7.89' 2019-07-05T17:22:51 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'sra-download.ncbi.nlm.nih.gov' (130.14.250.24) from '172.19.7.89' 2019-07-05T17:22:51 fasterq-dump.2.9.6 err: connection failed while opening file within cryptographic module - error with https open 'https://sra-download.ncbi.nlm.nih.gov/sos/sra-pub-run-1/SRR8207203/SRR8207203.1' 2019-07-05T17:22:51 fasterq-dump.2.9.6 err: cmn_iter.c cmn_get_db_type().VDBManagerOpenDBRead( 'SRR8207203' ) -> RC(rcKrypto,rcFile,rcOpening,rcConnection,rcFailed) 2019-07-05T17:22:51 fasterq-dump.2.9.6 err: invalid accession 'SRR8207203' spots read : 8,441,689 reads read : 16,883,378 reads written : 8,441,689 reads 0-length : 8,441,689 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:43 8441689 reads; of these: 8441689 (100.00%) were unpaired; of these: 261182 (3.09%) aligned 0 times 6907231 (81.82%) aligned exactly 1 time 1273276 (15.08%) aligned >1 times 96.91% overall alignment rate Time searching: 00:02:43 Overall time: 00:02:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2953557 / 8180507 = 0.3610 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 02:36:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5026525/SRX5026525.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5026525/SRX5026525.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5026525/SRX5026525.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5026525/SRX5026525.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:36:12: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:36:12: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:36:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5026525/SRX5026525.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5026525/SRX5026525.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5026525/SRX5026525.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5026525/SRX5026525.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:36:13: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:36:13: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:36:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5026525/SRX5026525.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5026525/SRX5026525.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5026525/SRX5026525.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5026525/SRX5026525.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:36:14: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:36:14: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:36:21: 1000000 INFO @ Sat, 06 Jul 2019 02:36:25: 1000000 INFO @ Sat, 06 Jul 2019 02:36:25: 1000000 INFO @ Sat, 06 Jul 2019 02:36:30: 2000000 INFO @ Sat, 06 Jul 2019 02:36:35: 2000000 INFO @ Sat, 06 Jul 2019 02:36:37: 2000000 INFO @ Sat, 06 Jul 2019 02:36:38: 3000000 INFO @ Sat, 06 Jul 2019 02:36:44: 3000000 INFO @ Sat, 06 Jul 2019 02:36:46: 4000000 INFO @ Sat, 06 Jul 2019 02:36:48: 3000000 INFO @ Sat, 06 Jul 2019 02:36:54: 4000000 INFO @ Sat, 06 Jul 2019 02:36:55: 5000000 INFO @ Sat, 06 Jul 2019 02:36:56: #1 tag size is determined as 101 bps INFO @ Sat, 06 Jul 2019 02:36:56: #1 tag size = 101 INFO @ Sat, 06 Jul 2019 02:36:56: #1 total tags in treatment: 5226950 INFO @ Sat, 06 Jul 2019 02:36:56: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:36:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:36:57: #1 tags after filtering in treatment: 5226950 INFO @ Sat, 06 Jul 2019 02:36:57: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:36:57: #1 finished! INFO @ Sat, 06 Jul 2019 02:36:57: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:36:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:36:57: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:36:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:36:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5026525/SRX5026525.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5026525/SRX5026525.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5026525/SRX5026525.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5026525/SRX5026525.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 02:36:59: 4000000 INFO @ Sat, 06 Jul 2019 02:37:03: 5000000 INFO @ Sat, 06 Jul 2019 02:37:05: #1 tag size is determined as 101 bps INFO @ Sat, 06 Jul 2019 02:37:05: #1 tag size = 101 INFO @ Sat, 06 Jul 2019 02:37:05: #1 total tags in treatment: 5226950 INFO @ Sat, 06 Jul 2019 02:37:05: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:37:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:37:05: #1 tags after filtering in treatment: 5226950 INFO @ Sat, 06 Jul 2019 02:37:05: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:37:05: #1 finished! INFO @ Sat, 06 Jul 2019 02:37:05: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:37:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:37:06: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:37:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:37:06: Process for pairing-model is terminated! INFO @ Sat, 06 Jul 2019 02:37:09: 5000000 INFO @ Sat, 06 Jul 2019 02:37:12: #1 tag size is determined as 101 bps INFO @ Sat, 06 Jul 2019 02:37:12: #1 tag size = 101 INFO @ Sat, 06 Jul 2019 02:37:12: #1 total tags in treatment: 5226950 INFO @ Sat, 06 Jul 2019 02:37:12: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:37:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:37:12: #1 tags after filtering in treatment: 5226950 INFO @ Sat, 06 Jul 2019 02:37:12: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:37:12: #1 finished! INFO @ Sat, 06 Jul 2019 02:37:12: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:37:12: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:37:12: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:37:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:37:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5026525/SRX5026525.20_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/SRX5026525/SRX5026525.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5026525/SRX5026525.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5026525/SRX5026525.20_*.xls’: No such file or directoryrm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5026525/SRX5026525.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5026525/SRX5026525.20_peaks.narrowPeak’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5026525/SRX5026525.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5026525/SRX5026525.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。