Job ID = 11635056 sra ファイルのダウンロード中... Completed: 906618K bytes transferred in 10 seconds (739047K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... spots read : 40,707,438 reads read : 81,414,876 reads written : 40,707,438 reads 0-length : 40,707,438 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:51 40707438 reads; of these: 40707438 (100.00%) were unpaired; of these: 2195029 (5.39%) aligned 0 times 26059327 (64.02%) aligned exactly 1 time 12453082 (30.59%) aligned >1 times 94.61% overall alignment rate Time searching: 00:02:51 Overall time: 00:02:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 23243147 / 38512409 = 0.6035 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 15:18:15: # Command line: callpeak -t SRX5019432.bam -f BAM -g 12100000 -n SRX5019432.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX5019432.20 # format = BAM # ChIP-seq file = ['SRX5019432.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 15:18:15: # Command line: callpeak -t SRX5019432.bam -f BAM -g 12100000 -n SRX5019432.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX5019432.10 # format = BAM # ChIP-seq file = ['SRX5019432.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 15:18:15: # Command line: callpeak -t SRX5019432.bam -f BAM -g 12100000 -n SRX5019432.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX5019432.05 # format = BAM # ChIP-seq file = ['SRX5019432.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 15:18:15: #1 read tag files... INFO @ Fri, 15 Feb 2019 15:18:15: #1 read tag files... INFO @ Fri, 15 Feb 2019 15:18:15: #1 read tag files... INFO @ Fri, 15 Feb 2019 15:18:15: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 15:18:15: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 15:18:15: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 15:18:22: 1000000 INFO @ Fri, 15 Feb 2019 15:18:22: 1000000 INFO @ Fri, 15 Feb 2019 15:18:23: 1000000 INFO @ Fri, 15 Feb 2019 15:18:29: 2000000 INFO @ Fri, 15 Feb 2019 15:18:29: 2000000 INFO @ Fri, 15 Feb 2019 15:18:30: 2000000 INFO @ Fri, 15 Feb 2019 15:18:35: 3000000 INFO @ Fri, 15 Feb 2019 15:18:36: 3000000 INFO @ Fri, 15 Feb 2019 15:18:37: 3000000 INFO @ Fri, 15 Feb 2019 15:18:42: 4000000 INFO @ Fri, 15 Feb 2019 15:18:43: 4000000 INFO @ Fri, 15 Feb 2019 15:18:45: 4000000 INFO @ Fri, 15 Feb 2019 15:18:50: 5000000 INFO @ Fri, 15 Feb 2019 15:18:51: 5000000 INFO @ Fri, 15 Feb 2019 15:18:53: 5000000 INFO @ Fri, 15 Feb 2019 15:18:58: 6000000 INFO @ Fri, 15 Feb 2019 15:18:58: 6000000 INFO @ Fri, 15 Feb 2019 15:19:02: 6000000 INFO @ Fri, 15 Feb 2019 15:19:06: 7000000 INFO @ Fri, 15 Feb 2019 15:19:06: 7000000 INFO @ Fri, 15 Feb 2019 15:19:10: 7000000 INFO @ Fri, 15 Feb 2019 15:19:13: 8000000 INFO @ Fri, 15 Feb 2019 15:19:13: 8000000 INFO @ Fri, 15 Feb 2019 15:19:18: 8000000 INFO @ Fri, 15 Feb 2019 15:19:21: 9000000 INFO @ Fri, 15 Feb 2019 15:19:21: 9000000 INFO @ Fri, 15 Feb 2019 15:19:26: 9000000 INFO @ Fri, 15 Feb 2019 15:19:28: 10000000 INFO @ Fri, 15 Feb 2019 15:19:29: 10000000 INFO @ Fri, 15 Feb 2019 15:19:34: 10000000 INFO @ Fri, 15 Feb 2019 15:19:36: 11000000 INFO @ Fri, 15 Feb 2019 15:19:38: 11000000 INFO @ Fri, 15 Feb 2019 15:19:42: 11000000 INFO @ Fri, 15 Feb 2019 15:19:44: 12000000 INFO @ Fri, 15 Feb 2019 15:19:46: 12000000 INFO @ Fri, 15 Feb 2019 15:19:50: 12000000 INFO @ Fri, 15 Feb 2019 15:19:51: 13000000 INFO @ Fri, 15 Feb 2019 15:19:53: 13000000 INFO @ Fri, 15 Feb 2019 15:19:57: 13000000 INFO @ Fri, 15 Feb 2019 15:19:58: 14000000 INFO @ Fri, 15 Feb 2019 15:20:00: 14000000 INFO @ Fri, 15 Feb 2019 15:20:04: 14000000 INFO @ Fri, 15 Feb 2019 15:20:05: 15000000 INFO @ Fri, 15 Feb 2019 15:20:07: #1 tag size is determined as 50 bps INFO @ Fri, 15 Feb 2019 15:20:07: #1 tag size = 50 INFO @ Fri, 15 Feb 2019 15:20:07: #1 total tags in treatment: 15269262 INFO @ Fri, 15 Feb 2019 15:20:07: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 15:20:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 15:20:07: 15000000 INFO @ Fri, 15 Feb 2019 15:20:07: #1 tags after filtering in treatment: 15269262 INFO @ Fri, 15 Feb 2019 15:20:07: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 15:20:07: #1 finished! INFO @ Fri, 15 Feb 2019 15:20:07: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 15:20:07: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 15:20:08: #2 number of paired peaks: 0 WARNING @ Fri, 15 Feb 2019 15:20:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 15:20:08: Process for pairing-model is terminated! cat: SRX5019432.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 4 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX5019432.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX5019432.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX5019432.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 15:20:09: #1 tag size is determined as 50 bps INFO @ Fri, 15 Feb 2019 15:20:09: #1 tag size = 50 INFO @ Fri, 15 Feb 2019 15:20:09: #1 total tags in treatment: 15269262 INFO @ Fri, 15 Feb 2019 15:20:09: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 15:20:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 15:20:09: #1 tags after filtering in treatment: 15269262 INFO @ Fri, 15 Feb 2019 15:20:09: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 15:20:09: #1 finished! INFO @ Fri, 15 Feb 2019 15:20:09: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 15:20:09: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 15:20:10: #2 number of paired peaks: 0 WARNING @ Fri, 15 Feb 2019 15:20:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 15:20:10: Process for pairing-model is terminated! cat: SRX5019432.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX5019432.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX5019432.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX5019432.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 15:20:11: 15000000 INFO @ Fri, 15 Feb 2019 15:20:13: #1 tag size is determined as 50 bps INFO @ Fri, 15 Feb 2019 15:20:13: #1 tag size = 50 INFO @ Fri, 15 Feb 2019 15:20:13: #1 total tags in treatment: 15269262 INFO @ Fri, 15 Feb 2019 15:20:13: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 15:20:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 15:20:13: #1 tags after filtering in treatment: 15269262 INFO @ Fri, 15 Feb 2019 15:20:13: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 15:20:13: #1 finished! INFO @ Fri, 15 Feb 2019 15:20:13: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 15:20:13: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 15:20:14: #2 number of paired peaks: 0 WARNING @ Fri, 15 Feb 2019 15:20:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 15:20:14: Process for pairing-model is terminated! cat: SRX5019432.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 6 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX5019432.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX5019432.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX5019432.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。