Job ID = 11634727 sra ファイルのダウンロード中... Completed: 616447K bytes transferred in 12 seconds (416964K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 29780600 spots for /home/okishinya/chipatlas/results/sacCer3/SRX5019426/SRR8200049.sra Written 29780600 spots for /home/okishinya/chipatlas/results/sacCer3/SRX5019426/SRR8200049.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:51 29780600 reads; of these: 29780600 (100.00%) were unpaired; of these: 1711518 (5.75%) aligned 0 times 20995094 (70.50%) aligned exactly 1 time 7073988 (23.75%) aligned >1 times 94.25% overall alignment rate Time searching: 00:04:51 Overall time: 00:04:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 14570653 / 28069082 = 0.5191 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 10:43:02: # Command line: callpeak -t SRX5019426.bam -f BAM -g 12100000 -n SRX5019426.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX5019426.20 # format = BAM # ChIP-seq file = ['SRX5019426.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 10:43:02: #1 read tag files... INFO @ Fri, 15 Feb 2019 10:43:02: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 10:43:02: # Command line: callpeak -t SRX5019426.bam -f BAM -g 12100000 -n SRX5019426.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX5019426.10 # format = BAM # ChIP-seq file = ['SRX5019426.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 10:43:02: #1 read tag files... INFO @ Fri, 15 Feb 2019 10:43:02: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 10:43:02: # Command line: callpeak -t SRX5019426.bam -f BAM -g 12100000 -n SRX5019426.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX5019426.05 # format = BAM # ChIP-seq file = ['SRX5019426.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 10:43:02: #1 read tag files... INFO @ Fri, 15 Feb 2019 10:43:02: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 10:43:09: 1000000 INFO @ Fri, 15 Feb 2019 10:43:10: 1000000 INFO @ Fri, 15 Feb 2019 10:43:10: 1000000 INFO @ Fri, 15 Feb 2019 10:43:15: 2000000 INFO @ Fri, 15 Feb 2019 10:43:17: 2000000 INFO @ Fri, 15 Feb 2019 10:43:17: 2000000 INFO @ Fri, 15 Feb 2019 10:43:22: 3000000 INFO @ Fri, 15 Feb 2019 10:43:25: 3000000 INFO @ Fri, 15 Feb 2019 10:43:25: 3000000 INFO @ Fri, 15 Feb 2019 10:43:28: 4000000 INFO @ Fri, 15 Feb 2019 10:43:32: 4000000 INFO @ Fri, 15 Feb 2019 10:43:32: 4000000 INFO @ Fri, 15 Feb 2019 10:43:35: 5000000 INFO @ Fri, 15 Feb 2019 10:43:39: 5000000 INFO @ Fri, 15 Feb 2019 10:43:39: 5000000 INFO @ Fri, 15 Feb 2019 10:43:41: 6000000 INFO @ Fri, 15 Feb 2019 10:43:45: 6000000 INFO @ Fri, 15 Feb 2019 10:43:45: 6000000 INFO @ Fri, 15 Feb 2019 10:43:48: 7000000 INFO @ Fri, 15 Feb 2019 10:43:52: 7000000 INFO @ Fri, 15 Feb 2019 10:43:52: 7000000 INFO @ Fri, 15 Feb 2019 10:43:54: 8000000 INFO @ Fri, 15 Feb 2019 10:43:59: 8000000 INFO @ Fri, 15 Feb 2019 10:43:59: 8000000 INFO @ Fri, 15 Feb 2019 10:44:01: 9000000 INFO @ Fri, 15 Feb 2019 10:44:05: 9000000 INFO @ Fri, 15 Feb 2019 10:44:05: 9000000 INFO @ Fri, 15 Feb 2019 10:44:08: 10000000 INFO @ Fri, 15 Feb 2019 10:44:11: 10000000 INFO @ Fri, 15 Feb 2019 10:44:12: 10000000 INFO @ Fri, 15 Feb 2019 10:44:15: 11000000 INFO @ Fri, 15 Feb 2019 10:44:18: 11000000 INFO @ Fri, 15 Feb 2019 10:44:19: 11000000 INFO @ Fri, 15 Feb 2019 10:44:23: 12000000 INFO @ Fri, 15 Feb 2019 10:44:24: 12000000 INFO @ Fri, 15 Feb 2019 10:44:26: 12000000 INFO @ Fri, 15 Feb 2019 10:44:30: 13000000 INFO @ Fri, 15 Feb 2019 10:44:31: 13000000 INFO @ Fri, 15 Feb 2019 10:44:33: 13000000 INFO @ Fri, 15 Feb 2019 10:44:34: #1 tag size is determined as 50 bps INFO @ Fri, 15 Feb 2019 10:44:34: #1 tag size = 50 INFO @ Fri, 15 Feb 2019 10:44:34: #1 total tags in treatment: 13498429 INFO @ Fri, 15 Feb 2019 10:44:34: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 10:44:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 10:44:34: #1 tags after filtering in treatment: 13498429 INFO @ Fri, 15 Feb 2019 10:44:34: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 10:44:34: #1 finished! INFO @ Fri, 15 Feb 2019 10:44:34: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 10:44:34: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 10:44:34: #1 tag size is determined as 50 bps INFO @ Fri, 15 Feb 2019 10:44:34: #1 tag size = 50 INFO @ Fri, 15 Feb 2019 10:44:34: #1 total tags in treatment: 13498429 INFO @ Fri, 15 Feb 2019 10:44:34: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 10:44:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 10:44:34: #1 tags after filtering in treatment: 13498429 INFO @ Fri, 15 Feb 2019 10:44:34: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 10:44:34: #1 finished! INFO @ Fri, 15 Feb 2019 10:44:34: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 10:44:34: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 10:44:35: #2 number of paired peaks: 0 WARNING @ Fri, 15 Feb 2019 10:44:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 10:44:35: Process for pairing-model is terminated! cat: SRX5019426.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX5019426.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX5019426.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX5019426.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 10:44:35: #2 number of paired peaks: 0 WARNING @ Fri, 15 Feb 2019 10:44:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 10:44:35: Process for pairing-model is terminated! cat: SRX5019426.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 6 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX5019426.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX5019426.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX5019426.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 10:44:36: #1 tag size is determined as 50 bps INFO @ Fri, 15 Feb 2019 10:44:36: #1 tag size = 50 INFO @ Fri, 15 Feb 2019 10:44:36: #1 total tags in treatment: 13498429 INFO @ Fri, 15 Feb 2019 10:44:36: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 10:44:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 10:44:36: #1 tags after filtering in treatment: 13498429 INFO @ Fri, 15 Feb 2019 10:44:36: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 10:44:36: #1 finished! INFO @ Fri, 15 Feb 2019 10:44:36: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 10:44:36: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 10:44:37: #2 number of paired peaks: 0 WARNING @ Fri, 15 Feb 2019 10:44:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 10:44:37: Process for pairing-model is terminated! cat: SRX5019426.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 4 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX5019426.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX5019426.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX5019426.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。