Job ID = 11634726 sra ファイルのダウンロード中... Completed: 381555K bytes transferred in 7 seconds (434532K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 18292159 spots for /home/okishinya/chipatlas/results/sacCer3/SRX5019425/SRR8200048.sra Written 18292159 spots for /home/okishinya/chipatlas/results/sacCer3/SRX5019425/SRR8200048.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:41 18292159 reads; of these: 18292159 (100.00%) were unpaired; of these: 5935732 (32.45%) aligned 0 times 8628097 (47.17%) aligned exactly 1 time 3728330 (20.38%) aligned >1 times 67.55% overall alignment rate Time searching: 00:02:41 Overall time: 00:02:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6263060 / 12356427 = 0.5069 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 10:36:57: # Command line: callpeak -t SRX5019425.bam -f BAM -g 12100000 -n SRX5019425.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX5019425.05 # format = BAM # ChIP-seq file = ['SRX5019425.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 10:36:57: #1 read tag files... INFO @ Fri, 15 Feb 2019 10:36:57: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 10:36:57: # Command line: callpeak -t SRX5019425.bam -f BAM -g 12100000 -n SRX5019425.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX5019425.20 # format = BAM # ChIP-seq file = ['SRX5019425.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 10:36:57: #1 read tag files... INFO @ Fri, 15 Feb 2019 10:36:57: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 10:36:57: # Command line: callpeak -t SRX5019425.bam -f BAM -g 12100000 -n SRX5019425.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX5019425.10 # format = BAM # ChIP-seq file = ['SRX5019425.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 10:36:57: #1 read tag files... INFO @ Fri, 15 Feb 2019 10:36:57: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 10:37:05: 1000000 INFO @ Fri, 15 Feb 2019 10:37:05: 1000000 INFO @ Fri, 15 Feb 2019 10:37:05: 1000000 INFO @ Fri, 15 Feb 2019 10:37:12: 2000000 INFO @ Fri, 15 Feb 2019 10:37:13: 2000000 INFO @ Fri, 15 Feb 2019 10:37:13: 2000000 INFO @ Fri, 15 Feb 2019 10:37:19: 3000000 INFO @ Fri, 15 Feb 2019 10:37:21: 3000000 INFO @ Fri, 15 Feb 2019 10:37:21: 3000000 INFO @ Fri, 15 Feb 2019 10:37:26: 4000000 INFO @ Fri, 15 Feb 2019 10:37:28: 4000000 INFO @ Fri, 15 Feb 2019 10:37:28: 4000000 INFO @ Fri, 15 Feb 2019 10:37:32: 5000000 INFO @ Fri, 15 Feb 2019 10:37:36: 5000000 INFO @ Fri, 15 Feb 2019 10:37:36: 5000000 INFO @ Fri, 15 Feb 2019 10:37:40: 6000000 INFO @ Fri, 15 Feb 2019 10:37:40: #1 tag size is determined as 50 bps INFO @ Fri, 15 Feb 2019 10:37:40: #1 tag size = 50 INFO @ Fri, 15 Feb 2019 10:37:40: #1 total tags in treatment: 6093367 INFO @ Fri, 15 Feb 2019 10:37:40: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 10:37:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 10:37:40: #1 tags after filtering in treatment: 6093367 INFO @ Fri, 15 Feb 2019 10:37:40: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 10:37:40: #1 finished! INFO @ Fri, 15 Feb 2019 10:37:40: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 10:37:40: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 10:37:41: #2 number of paired peaks: 0 WARNING @ Fri, 15 Feb 2019 10:37:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 10:37:41: Process for pairing-model is terminated! cat: SRX5019425.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 6 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX5019425.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX5019425.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX5019425.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 10:37:43: 6000000 INFO @ Fri, 15 Feb 2019 10:37:43: 6000000 INFO @ Fri, 15 Feb 2019 10:37:44: #1 tag size is determined as 50 bps INFO @ Fri, 15 Feb 2019 10:37:44: #1 tag size = 50 INFO @ Fri, 15 Feb 2019 10:37:44: #1 total tags in treatment: 6093367 INFO @ Fri, 15 Feb 2019 10:37:44: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 10:37:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 10:37:44: #1 tag size is determined as 50 bps INFO @ Fri, 15 Feb 2019 10:37:44: #1 tag size = 50 INFO @ Fri, 15 Feb 2019 10:37:44: #1 total tags in treatment: 6093367 INFO @ Fri, 15 Feb 2019 10:37:44: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 10:37:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 10:37:44: #1 tags after filtering in treatment: 6093367 INFO @ Fri, 15 Feb 2019 10:37:44: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 10:37:44: #1 finished! INFO @ Fri, 15 Feb 2019 10:37:44: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 10:37:44: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 10:37:44: #1 tags after filtering in treatment: 6093367 INFO @ Fri, 15 Feb 2019 10:37:44: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 10:37:44: #1 finished! INFO @ Fri, 15 Feb 2019 10:37:44: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 10:37:44: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 10:37:44: #2 number of paired peaks: 0 WARNING @ Fri, 15 Feb 2019 10:37:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 10:37:44: Process for pairing-model is terminated! cat: SRX5019425.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 6 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX5019425.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX5019425.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX5019425.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 10:37:45: #2 number of paired peaks: 0 WARNING @ Fri, 15 Feb 2019 10:37:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 10:37:45: Process for pairing-model is terminated! cat: SRX5019425.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 10 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX5019425.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX5019425.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX5019425.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。