Job ID = 11634717 sra ファイルのダウンロード中... Completed: 699074K bytes transferred in 9 seconds (576822K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 38037003 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4957452/SRR8136466.sra Written 38037003 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4957452/SRR8136466.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:48 38037003 reads; of these: 38037003 (100.00%) were unpaired; of these: 3473049 (9.13%) aligned 0 times 31042039 (81.61%) aligned exactly 1 time 3521915 (9.26%) aligned >1 times 90.87% overall alignment rate Time searching: 00:06:48 Overall time: 00:06:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 21723024 / 34563954 = 0.6285 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 10:53:19: # Command line: callpeak -t SRX4957452.bam -f BAM -g 12100000 -n SRX4957452.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4957452.10 # format = BAM # ChIP-seq file = ['SRX4957452.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 10:53:19: #1 read tag files... INFO @ Fri, 15 Feb 2019 10:53:19: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 10:53:19: # Command line: callpeak -t SRX4957452.bam -f BAM -g 12100000 -n SRX4957452.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4957452.20 # format = BAM # ChIP-seq file = ['SRX4957452.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 10:53:19: #1 read tag files... INFO @ Fri, 15 Feb 2019 10:53:19: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 10:53:19: # Command line: callpeak -t SRX4957452.bam -f BAM -g 12100000 -n SRX4957452.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4957452.05 # format = BAM # ChIP-seq file = ['SRX4957452.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 10:53:19: #1 read tag files... INFO @ Fri, 15 Feb 2019 10:53:19: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 10:53:26: 1000000 INFO @ Fri, 15 Feb 2019 10:53:26: 1000000 INFO @ Fri, 15 Feb 2019 10:53:26: 1000000 INFO @ Fri, 15 Feb 2019 10:53:32: 2000000 INFO @ Fri, 15 Feb 2019 10:53:32: 2000000 INFO @ Fri, 15 Feb 2019 10:53:33: 2000000 INFO @ Fri, 15 Feb 2019 10:53:38: 3000000 INFO @ Fri, 15 Feb 2019 10:53:38: 3000000 INFO @ Fri, 15 Feb 2019 10:53:39: 3000000 INFO @ Fri, 15 Feb 2019 10:53:45: 4000000 INFO @ Fri, 15 Feb 2019 10:53:45: 4000000 INFO @ Fri, 15 Feb 2019 10:53:47: 4000000 INFO @ Fri, 15 Feb 2019 10:53:51: 5000000 INFO @ Fri, 15 Feb 2019 10:53:52: 5000000 INFO @ Fri, 15 Feb 2019 10:53:54: 5000000 INFO @ Fri, 15 Feb 2019 10:53:58: 6000000 INFO @ Fri, 15 Feb 2019 10:53:58: 6000000 INFO @ Fri, 15 Feb 2019 10:54:01: 6000000 INFO @ Fri, 15 Feb 2019 10:54:04: 7000000 INFO @ Fri, 15 Feb 2019 10:54:05: 7000000 INFO @ Fri, 15 Feb 2019 10:54:07: 7000000 INFO @ Fri, 15 Feb 2019 10:54:11: 8000000 INFO @ Fri, 15 Feb 2019 10:54:11: 8000000 INFO @ Fri, 15 Feb 2019 10:54:15: 8000000 INFO @ Fri, 15 Feb 2019 10:54:18: 9000000 INFO @ Fri, 15 Feb 2019 10:54:18: 9000000 INFO @ Fri, 15 Feb 2019 10:54:21: 9000000 INFO @ Fri, 15 Feb 2019 10:54:24: 10000000 INFO @ Fri, 15 Feb 2019 10:54:24: 10000000 INFO @ Fri, 15 Feb 2019 10:54:28: 10000000 INFO @ Fri, 15 Feb 2019 10:54:30: 11000000 INFO @ Fri, 15 Feb 2019 10:54:30: 11000000 INFO @ Fri, 15 Feb 2019 10:54:35: 11000000 INFO @ Fri, 15 Feb 2019 10:54:36: 12000000 INFO @ Fri, 15 Feb 2019 10:54:37: 12000000 INFO @ Fri, 15 Feb 2019 10:54:41: 12000000 INFO @ Fri, 15 Feb 2019 10:54:41: #1 tag size is determined as 50 bps INFO @ Fri, 15 Feb 2019 10:54:41: #1 tag size = 50 INFO @ Fri, 15 Feb 2019 10:54:41: #1 total tags in treatment: 12840930 INFO @ Fri, 15 Feb 2019 10:54:41: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 10:54:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 10:54:42: #1 tags after filtering in treatment: 12840930 INFO @ Fri, 15 Feb 2019 10:54:42: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 10:54:42: #1 finished! INFO @ Fri, 15 Feb 2019 10:54:42: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 10:54:42: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 10:54:42: #1 tag size is determined as 50 bps INFO @ Fri, 15 Feb 2019 10:54:42: #1 tag size = 50 INFO @ Fri, 15 Feb 2019 10:54:42: #1 total tags in treatment: 12840930 INFO @ Fri, 15 Feb 2019 10:54:42: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 10:54:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 10:54:42: #1 tags after filtering in treatment: 12840930 INFO @ Fri, 15 Feb 2019 10:54:42: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 10:54:42: #1 finished! INFO @ Fri, 15 Feb 2019 10:54:42: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 10:54:42: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 10:54:43: #2 number of paired peaks: 0 WARNING @ Fri, 15 Feb 2019 10:54:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 10:54:43: Process for pairing-model is terminated! cat: SRX4957452.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4957452.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4957452.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4957452.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 10:54:43: #2 number of paired peaks: 0 WARNING @ Fri, 15 Feb 2019 10:54:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 10:54:43: Process for pairing-model is terminated! cat: SRX4957452.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4957452.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4957452.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4957452.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 10:54:47: #1 tag size is determined as 50 bps INFO @ Fri, 15 Feb 2019 10:54:47: #1 tag size = 50 INFO @ Fri, 15 Feb 2019 10:54:47: #1 total tags in treatment: 12840930 INFO @ Fri, 15 Feb 2019 10:54:47: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 10:54:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 10:54:47: #1 tags after filtering in treatment: 12840930 INFO @ Fri, 15 Feb 2019 10:54:47: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 10:54:47: #1 finished! INFO @ Fri, 15 Feb 2019 10:54:47: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 10:54:47: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 10:54:48: #2 number of paired peaks: 0 WARNING @ Fri, 15 Feb 2019 10:54:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 10:54:48: Process for pairing-model is terminated! cat: SRX4957452.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4957452.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4957452.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4957452.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。