Job ID = 11634708 sra ファイルのダウンロード中... Completed: 697029K bytes transferred in 13 seconds (436946K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 38013051 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4957444/SRR8136458.sra Written 38013051 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4957444/SRR8136458.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:41 38013051 reads; of these: 38013051 (100.00%) were unpaired; of these: 2047528 (5.39%) aligned 0 times 32200014 (84.71%) aligned exactly 1 time 3765509 (9.91%) aligned >1 times 94.61% overall alignment rate Time searching: 00:06:41 Overall time: 00:06:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 22014339 / 35965523 = 0.6121 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 10:43:51: # Command line: callpeak -t SRX4957444.bam -f BAM -g 12100000 -n SRX4957444.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4957444.05 # format = BAM # ChIP-seq file = ['SRX4957444.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 10:43:51: #1 read tag files... INFO @ Fri, 15 Feb 2019 10:43:51: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 10:43:51: # Command line: callpeak -t SRX4957444.bam -f BAM -g 12100000 -n SRX4957444.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4957444.20 # format = BAM # ChIP-seq file = ['SRX4957444.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 10:43:51: #1 read tag files... INFO @ Fri, 15 Feb 2019 10:43:51: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 10:43:51: # Command line: callpeak -t SRX4957444.bam -f BAM -g 12100000 -n SRX4957444.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4957444.10 # format = BAM # ChIP-seq file = ['SRX4957444.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 10:43:51: #1 read tag files... INFO @ Fri, 15 Feb 2019 10:43:51: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 10:43:58: 1000000 INFO @ Fri, 15 Feb 2019 10:43:58: 1000000 INFO @ Fri, 15 Feb 2019 10:43:58: 1000000 INFO @ Fri, 15 Feb 2019 10:44:05: 2000000 INFO @ Fri, 15 Feb 2019 10:44:05: 2000000 INFO @ Fri, 15 Feb 2019 10:44:05: 2000000 INFO @ Fri, 15 Feb 2019 10:44:11: 3000000 INFO @ Fri, 15 Feb 2019 10:44:12: 3000000 INFO @ Fri, 15 Feb 2019 10:44:12: 3000000 INFO @ Fri, 15 Feb 2019 10:44:18: 4000000 INFO @ Fri, 15 Feb 2019 10:44:19: 4000000 INFO @ Fri, 15 Feb 2019 10:44:19: 4000000 INFO @ Fri, 15 Feb 2019 10:44:25: 5000000 INFO @ Fri, 15 Feb 2019 10:44:26: 5000000 INFO @ Fri, 15 Feb 2019 10:44:26: 5000000 INFO @ Fri, 15 Feb 2019 10:44:32: 6000000 INFO @ Fri, 15 Feb 2019 10:44:33: 6000000 INFO @ Fri, 15 Feb 2019 10:44:33: 6000000 INFO @ Fri, 15 Feb 2019 10:44:38: 7000000 INFO @ Fri, 15 Feb 2019 10:44:40: 7000000 INFO @ Fri, 15 Feb 2019 10:44:40: 7000000 INFO @ Fri, 15 Feb 2019 10:44:45: 8000000 INFO @ Fri, 15 Feb 2019 10:44:47: 8000000 INFO @ Fri, 15 Feb 2019 10:44:47: 8000000 INFO @ Fri, 15 Feb 2019 10:44:52: 9000000 INFO @ Fri, 15 Feb 2019 10:44:54: 9000000 INFO @ Fri, 15 Feb 2019 10:44:54: 9000000 INFO @ Fri, 15 Feb 2019 10:44:59: 10000000 INFO @ Fri, 15 Feb 2019 10:45:01: 10000000 INFO @ Fri, 15 Feb 2019 10:45:01: 10000000 INFO @ Fri, 15 Feb 2019 10:45:06: 11000000 INFO @ Fri, 15 Feb 2019 10:45:08: 11000000 INFO @ Fri, 15 Feb 2019 10:45:08: 11000000 INFO @ Fri, 15 Feb 2019 10:45:14: 12000000 INFO @ Fri, 15 Feb 2019 10:45:15: 12000000 INFO @ Fri, 15 Feb 2019 10:45:15: 12000000 INFO @ Fri, 15 Feb 2019 10:45:21: 13000000 INFO @ Fri, 15 Feb 2019 10:45:22: 13000000 INFO @ Fri, 15 Feb 2019 10:45:22: 13000000 INFO @ Fri, 15 Feb 2019 10:45:27: #1 tag size is determined as 50 bps INFO @ Fri, 15 Feb 2019 10:45:27: #1 tag size = 50 INFO @ Fri, 15 Feb 2019 10:45:27: #1 total tags in treatment: 13951184 INFO @ Fri, 15 Feb 2019 10:45:27: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 10:45:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 10:45:27: #1 tags after filtering in treatment: 13951184 INFO @ Fri, 15 Feb 2019 10:45:27: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 10:45:27: #1 finished! INFO @ Fri, 15 Feb 2019 10:45:27: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 10:45:27: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 10:45:28: #1 tag size is determined as 50 bps INFO @ Fri, 15 Feb 2019 10:45:28: #1 tag size = 50 INFO @ Fri, 15 Feb 2019 10:45:28: #1 total tags in treatment: 13951184 INFO @ Fri, 15 Feb 2019 10:45:28: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 10:45:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 10:45:28: #2 number of paired peaks: 0 WARNING @ Fri, 15 Feb 2019 10:45:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 10:45:28: Process for pairing-model is terminated! cat: SRX4957444.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 5 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4957444.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4957444.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4957444.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 10:45:28: #1 tags after filtering in treatment: 13951184 INFO @ Fri, 15 Feb 2019 10:45:28: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 10:45:28: #1 finished! INFO @ Fri, 15 Feb 2019 10:45:28: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 10:45:28: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 10:45:28: #1 tag size is determined as 50 bps INFO @ Fri, 15 Feb 2019 10:45:28: #1 tag size = 50 INFO @ Fri, 15 Feb 2019 10:45:28: #1 total tags in treatment: 13951184 INFO @ Fri, 15 Feb 2019 10:45:28: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 10:45:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 10:45:29: #1 tags after filtering in treatment: 13951184 INFO @ Fri, 15 Feb 2019 10:45:29: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 10:45:29: #1 finished! INFO @ Fri, 15 Feb 2019 10:45:29: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 10:45:29: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 10:45:29: #2 number of paired peaks: 0 WARNING @ Fri, 15 Feb 2019 10:45:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 10:45:29: Process for pairing-model is terminated! cat: SRX4957444.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 4 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4957444.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4957444.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4957444.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 10:45:29: #2 number of paired peaks: 0 WARNING @ Fri, 15 Feb 2019 10:45:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 10:45:29: Process for pairing-model is terminated! cat: SRX4957444.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 6 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4957444.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4957444.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4957444.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。