Job ID = 2640978 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 10,802,287 reads read : 21,604,574 reads written : 10,802,287 reads 0-length : 10,802,287 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:52 10802287 reads; of these: 10802287 (100.00%) were unpaired; of these: 743359 (6.88%) aligned 0 times 8668341 (80.25%) aligned exactly 1 time 1390587 (12.87%) aligned >1 times 93.12% overall alignment rate Time searching: 00:01:52 Overall time: 00:01:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2591743 / 10058928 = 0.2577 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 20:17:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4908724/SRX4908724.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4908724/SRX4908724.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4908724/SRX4908724.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4908724/SRX4908724.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 20:17:13: #1 read tag files... INFO @ Sat, 24 Aug 2019 20:17:13: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 20:17:22: 1000000 INFO @ Sat, 24 Aug 2019 20:17:31: 2000000 INFO @ Sat, 24 Aug 2019 20:17:40: 3000000 INFO @ Sat, 24 Aug 2019 20:17:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4908724/SRX4908724.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4908724/SRX4908724.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4908724/SRX4908724.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4908724/SRX4908724.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 20:17:42: #1 read tag files... INFO @ Sat, 24 Aug 2019 20:17:42: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 20:17:50: 4000000 INFO @ Sat, 24 Aug 2019 20:17:50: 1000000 INFO @ Sat, 24 Aug 2019 20:17:59: 2000000 INFO @ Sat, 24 Aug 2019 20:17:59: 5000000 INFO @ Sat, 24 Aug 2019 20:18:07: 3000000 INFO @ Sat, 24 Aug 2019 20:18:08: 6000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 20:18:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4908724/SRX4908724.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4908724/SRX4908724.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4908724/SRX4908724.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4908724/SRX4908724.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 20:18:12: #1 read tag files... INFO @ Sat, 24 Aug 2019 20:18:12: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 20:18:15: 4000000 INFO @ Sat, 24 Aug 2019 20:18:18: 7000000 INFO @ Sat, 24 Aug 2019 20:18:20: 1000000 INFO @ Sat, 24 Aug 2019 20:18:22: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 20:18:22: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 20:18:22: #1 total tags in treatment: 7467185 INFO @ Sat, 24 Aug 2019 20:18:22: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 20:18:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 20:18:22: #1 tags after filtering in treatment: 7467185 INFO @ Sat, 24 Aug 2019 20:18:22: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 20:18:22: #1 finished! INFO @ Sat, 24 Aug 2019 20:18:22: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 20:18:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 20:18:23: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 20:18:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 20:18:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4908724/SRX4908724.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4908724/SRX4908724.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4908724/SRX4908724.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4908724/SRX4908724.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 20:18:23: 5000000 INFO @ Sat, 24 Aug 2019 20:18:28: 2000000 INFO @ Sat, 24 Aug 2019 20:18:32: 6000000 INFO @ Sat, 24 Aug 2019 20:18:36: 3000000 INFO @ Sat, 24 Aug 2019 20:18:40: 7000000 INFO @ Sat, 24 Aug 2019 20:18:43: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 20:18:43: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 20:18:43: #1 total tags in treatment: 7467185 INFO @ Sat, 24 Aug 2019 20:18:43: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 20:18:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 20:18:43: #1 tags after filtering in treatment: 7467185 INFO @ Sat, 24 Aug 2019 20:18:43: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 20:18:43: #1 finished! INFO @ Sat, 24 Aug 2019 20:18:43: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 20:18:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 20:18:44: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 20:18:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 20:18:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4908724/SRX4908724.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4908724/SRX4908724.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4908724/SRX4908724.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4908724/SRX4908724.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 20:18:44: 4000000 INFO @ Sat, 24 Aug 2019 20:18:52: 5000000 INFO @ Sat, 24 Aug 2019 20:19:00: 6000000 INFO @ Sat, 24 Aug 2019 20:19:07: 7000000 INFO @ Sat, 24 Aug 2019 20:19:11: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 20:19:11: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 20:19:11: #1 total tags in treatment: 7467185 INFO @ Sat, 24 Aug 2019 20:19:11: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 20:19:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 20:19:11: #1 tags after filtering in treatment: 7467185 INFO @ Sat, 24 Aug 2019 20:19:11: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 20:19:11: #1 finished! INFO @ Sat, 24 Aug 2019 20:19:11: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 20:19:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 20:19:11: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 20:19:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 20:19:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4908724/SRX4908724.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4908724/SRX4908724.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4908724/SRX4908724.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4908724/SRX4908724.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。