Job ID = 2640976 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 7,566,214 reads read : 15,132,428 reads written : 7,566,214 reads 0-length : 7,566,214 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:29 7566214 reads; of these: 7566214 (100.00%) were unpaired; of these: 185615 (2.45%) aligned 0 times 6223463 (82.25%) aligned exactly 1 time 1157136 (15.29%) aligned >1 times 97.55% overall alignment rate Time searching: 00:01:29 Overall time: 00:01:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1566640 / 7380599 = 0.2123 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 20:15:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4908722/SRX4908722.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4908722/SRX4908722.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4908722/SRX4908722.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4908722/SRX4908722.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 20:15:14: #1 read tag files... INFO @ Sat, 24 Aug 2019 20:15:14: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 20:15:21: 1000000 INFO @ Sat, 24 Aug 2019 20:15:28: 2000000 INFO @ Sat, 24 Aug 2019 20:15:35: 3000000 INFO @ Sat, 24 Aug 2019 20:15:41: 4000000 INFO @ Sat, 24 Aug 2019 20:15:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4908722/SRX4908722.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4908722/SRX4908722.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4908722/SRX4908722.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4908722/SRX4908722.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 20:15:44: #1 read tag files... INFO @ Sat, 24 Aug 2019 20:15:44: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 20:15:47: 5000000 INFO @ Sat, 24 Aug 2019 20:15:52: 1000000 INFO @ Sat, 24 Aug 2019 20:15:53: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 20:15:53: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 20:15:53: #1 total tags in treatment: 5813959 INFO @ Sat, 24 Aug 2019 20:15:53: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 20:15:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 20:15:53: #1 tags after filtering in treatment: 5813959 INFO @ Sat, 24 Aug 2019 20:15:53: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 20:15:53: #1 finished! INFO @ Sat, 24 Aug 2019 20:15:53: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 20:15:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 20:15:53: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 20:15:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 20:15:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4908722/SRX4908722.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4908722/SRX4908722.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4908722/SRX4908722.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4908722/SRX4908722.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 20:16:00: 2000000 INFO @ Sat, 24 Aug 2019 20:16:08: 3000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 20:16:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4908722/SRX4908722.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4908722/SRX4908722.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4908722/SRX4908722.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4908722/SRX4908722.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 20:16:14: #1 read tag files... INFO @ Sat, 24 Aug 2019 20:16:14: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 20:16:15: 4000000 INFO @ Sat, 24 Aug 2019 20:16:21: 1000000 INFO @ Sat, 24 Aug 2019 20:16:23: 5000000 INFO @ Sat, 24 Aug 2019 20:16:29: 2000000 INFO @ Sat, 24 Aug 2019 20:16:29: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 20:16:29: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 20:16:29: #1 total tags in treatment: 5813959 INFO @ Sat, 24 Aug 2019 20:16:29: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 20:16:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 20:16:29: #1 tags after filtering in treatment: 5813959 INFO @ Sat, 24 Aug 2019 20:16:29: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 20:16:29: #1 finished! INFO @ Sat, 24 Aug 2019 20:16:29: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 20:16:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 20:16:30: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 20:16:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 20:16:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4908722/SRX4908722.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4908722/SRX4908722.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4908722/SRX4908722.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4908722/SRX4908722.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 20:16:36: 3000000 INFO @ Sat, 24 Aug 2019 20:16:43: 4000000 INFO @ Sat, 24 Aug 2019 20:16:49: 5000000 INFO @ Sat, 24 Aug 2019 20:16:55: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 20:16:55: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 20:16:55: #1 total tags in treatment: 5813959 INFO @ Sat, 24 Aug 2019 20:16:55: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 20:16:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 20:16:55: #1 tags after filtering in treatment: 5813959 INFO @ Sat, 24 Aug 2019 20:16:55: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 20:16:55: #1 finished! INFO @ Sat, 24 Aug 2019 20:16:55: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 20:16:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 20:16:56: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 20:16:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 20:16:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4908722/SRX4908722.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4908722/SRX4908722.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4908722/SRX4908722.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4908722/SRX4908722.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。