Job ID = 11245270 sra ファイルのダウンロード中... Completed: 1431921K bytes transferred in 16 seconds (711242K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 35170188 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4733480/SRR7896035.sra Written 35170188 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4733480/SRR7896035.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:04 35170188 reads; of these: 35170188 (100.00%) were unpaired; of these: 1110720 (3.16%) aligned 0 times 28590261 (81.29%) aligned exactly 1 time 5469207 (15.55%) aligned >1 times 96.84% overall alignment rate Time searching: 00:11:04 Overall time: 00:11:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 18064216 / 34059468 = 0.5304 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 10 Oct 2018 00:28:23: # Command line: callpeak -t SRX4733480.bam -f BAM -g 12100000 -n SRX4733480.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4733480.05 # format = BAM # ChIP-seq file = ['SRX4733480.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 10 Oct 2018 00:28:23: #1 read tag files... INFO @ Wed, 10 Oct 2018 00:28:23: #1 read treatment tags... INFO @ Wed, 10 Oct 2018 00:28:23: # Command line: callpeak -t SRX4733480.bam -f BAM -g 12100000 -n SRX4733480.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4733480.20 # format = BAM # ChIP-seq file = ['SRX4733480.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 10 Oct 2018 00:28:23: #1 read tag files... INFO @ Wed, 10 Oct 2018 00:28:23: #1 read treatment tags... INFO @ Wed, 10 Oct 2018 00:28:23: # Command line: callpeak -t SRX4733480.bam -f BAM -g 12100000 -n SRX4733480.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4733480.10 # format = BAM # ChIP-seq file = ['SRX4733480.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 10 Oct 2018 00:28:23: #1 read tag files... INFO @ Wed, 10 Oct 2018 00:28:23: #1 read treatment tags... INFO @ Wed, 10 Oct 2018 00:28:31: 1000000 INFO @ Wed, 10 Oct 2018 00:28:31: 1000000 INFO @ Wed, 10 Oct 2018 00:28:31: 1000000 INFO @ Wed, 10 Oct 2018 00:28:39: 2000000 INFO @ Wed, 10 Oct 2018 00:28:39: 2000000 INFO @ Wed, 10 Oct 2018 00:28:39: 2000000 INFO @ Wed, 10 Oct 2018 00:28:46: 3000000 INFO @ Wed, 10 Oct 2018 00:28:47: 3000000 INFO @ Wed, 10 Oct 2018 00:28:47: 3000000 INFO @ Wed, 10 Oct 2018 00:28:54: 4000000 INFO @ Wed, 10 Oct 2018 00:28:55: 4000000 INFO @ Wed, 10 Oct 2018 00:28:55: 4000000 INFO @ Wed, 10 Oct 2018 00:29:01: 5000000 INFO @ Wed, 10 Oct 2018 00:29:02: 5000000 INFO @ Wed, 10 Oct 2018 00:29:02: 5000000 INFO @ Wed, 10 Oct 2018 00:29:09: 6000000 INFO @ Wed, 10 Oct 2018 00:29:10: 6000000 INFO @ Wed, 10 Oct 2018 00:29:10: 6000000 INFO @ Wed, 10 Oct 2018 00:29:16: 7000000 INFO @ Wed, 10 Oct 2018 00:29:18: 7000000 INFO @ Wed, 10 Oct 2018 00:29:18: 7000000 INFO @ Wed, 10 Oct 2018 00:29:24: 8000000 INFO @ Wed, 10 Oct 2018 00:29:25: 8000000 INFO @ Wed, 10 Oct 2018 00:29:25: 8000000 INFO @ Wed, 10 Oct 2018 00:29:31: 9000000 INFO @ Wed, 10 Oct 2018 00:29:33: 9000000 INFO @ Wed, 10 Oct 2018 00:29:33: 9000000 INFO @ Wed, 10 Oct 2018 00:29:39: 10000000 INFO @ Wed, 10 Oct 2018 00:29:41: 10000000 INFO @ Wed, 10 Oct 2018 00:29:41: 10000000 INFO @ Wed, 10 Oct 2018 00:29:46: 11000000 INFO @ Wed, 10 Oct 2018 00:29:49: 11000000 INFO @ Wed, 10 Oct 2018 00:29:49: 11000000 INFO @ Wed, 10 Oct 2018 00:29:54: 12000000 INFO @ Wed, 10 Oct 2018 00:29:56: 12000000 INFO @ Wed, 10 Oct 2018 00:29:56: 12000000 INFO @ Wed, 10 Oct 2018 00:30:01: 13000000 INFO @ Wed, 10 Oct 2018 00:30:04: 13000000 INFO @ Wed, 10 Oct 2018 00:30:04: 13000000 INFO @ Wed, 10 Oct 2018 00:30:09: 14000000 INFO @ Wed, 10 Oct 2018 00:30:12: 14000000 INFO @ Wed, 10 Oct 2018 00:30:12: 14000000 INFO @ Wed, 10 Oct 2018 00:30:16: 15000000 INFO @ Wed, 10 Oct 2018 00:30:19: 15000000 INFO @ Wed, 10 Oct 2018 00:30:19: 15000000 INFO @ Wed, 10 Oct 2018 00:30:24: #1 tag size is determined as 101 bps INFO @ Wed, 10 Oct 2018 00:30:24: #1 tag size = 101 INFO @ Wed, 10 Oct 2018 00:30:24: #1 total tags in treatment: 15995252 INFO @ Wed, 10 Oct 2018 00:30:24: #1 user defined the maximum tags... INFO @ Wed, 10 Oct 2018 00:30:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 10 Oct 2018 00:30:24: #1 tags after filtering in treatment: 15995252 INFO @ Wed, 10 Oct 2018 00:30:24: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 10 Oct 2018 00:30:24: #1 finished! INFO @ Wed, 10 Oct 2018 00:30:24: #2 Build Peak Model... INFO @ Wed, 10 Oct 2018 00:30:24: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 10 Oct 2018 00:30:25: #2 number of paired peaks: 0 WARNING @ Wed, 10 Oct 2018 00:30:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 10 Oct 2018 00:30:25: Process for pairing-model is terminated! cat: SRX4733480.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4733480.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4733480.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4733480.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 10 Oct 2018 00:30:27: #1 tag size is determined as 101 bps INFO @ Wed, 10 Oct 2018 00:30:27: #1 tag size = 101 INFO @ Wed, 10 Oct 2018 00:30:27: #1 total tags in treatment: 15995252 INFO @ Wed, 10 Oct 2018 00:30:27: #1 user defined the maximum tags... INFO @ Wed, 10 Oct 2018 00:30:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 10 Oct 2018 00:30:27: #1 tag size is determined as 101 bps INFO @ Wed, 10 Oct 2018 00:30:27: #1 tag size = 101 INFO @ Wed, 10 Oct 2018 00:30:27: #1 total tags in treatment: 15995252 INFO @ Wed, 10 Oct 2018 00:30:27: #1 user defined the maximum tags... INFO @ Wed, 10 Oct 2018 00:30:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 10 Oct 2018 00:30:27: #1 tags after filtering in treatment: 15995252 INFO @ Wed, 10 Oct 2018 00:30:27: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 10 Oct 2018 00:30:27: #1 finished! INFO @ Wed, 10 Oct 2018 00:30:27: #2 Build Peak Model... INFO @ Wed, 10 Oct 2018 00:30:27: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 10 Oct 2018 00:30:27: #1 tags after filtering in treatment: 15995252 INFO @ Wed, 10 Oct 2018 00:30:27: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 10 Oct 2018 00:30:27: #1 finished! INFO @ Wed, 10 Oct 2018 00:30:27: #2 Build Peak Model... INFO @ Wed, 10 Oct 2018 00:30:27: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 10 Oct 2018 00:30:28: #2 number of paired peaks: 0 WARNING @ Wed, 10 Oct 2018 00:30:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 10 Oct 2018 00:30:28: Process for pairing-model is terminated! INFO @ Wed, 10 Oct 2018 00:30:28: #2 number of paired peaks: 0 WARNING @ Wed, 10 Oct 2018 00:30:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 10 Oct 2018 00:30:28: Process for pairing-model is terminated! cat: SRX4733480.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) cat: SRX4733480.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません rm: cannot remove `SRX4733480.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4733480.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4733480.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4733480.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4733480.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4733480.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。