Job ID = 11245253 sra ファイルのダウンロード中... Completed: 200464K bytes transferred in 5 seconds (302429K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 4727744 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4733463/SRR7896018.sra Written 4727744 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4733463/SRR7896018.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:00 4727744 reads; of these: 4727744 (100.00%) were unpaired; of these: 1649734 (34.89%) aligned 0 times 1682849 (35.60%) aligned exactly 1 time 1395161 (29.51%) aligned >1 times 65.11% overall alignment rate Time searching: 00:01:00 Overall time: 00:01:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2394499 / 3078010 = 0.7779 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 10 Oct 2018 00:05:49: # Command line: callpeak -t SRX4733463.bam -f BAM -g 12100000 -n SRX4733463.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4733463.10 # format = BAM # ChIP-seq file = ['SRX4733463.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 10 Oct 2018 00:05:49: #1 read tag files... INFO @ Wed, 10 Oct 2018 00:05:49: #1 read treatment tags... INFO @ Wed, 10 Oct 2018 00:05:49: # Command line: callpeak -t SRX4733463.bam -f BAM -g 12100000 -n SRX4733463.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4733463.20 # format = BAM # ChIP-seq file = ['SRX4733463.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 10 Oct 2018 00:05:49: #1 read tag files... INFO @ Wed, 10 Oct 2018 00:05:49: #1 read treatment tags... INFO @ Wed, 10 Oct 2018 00:05:49: # Command line: callpeak -t SRX4733463.bam -f BAM -g 12100000 -n SRX4733463.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4733463.05 # format = BAM # ChIP-seq file = ['SRX4733463.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 10 Oct 2018 00:05:49: #1 read tag files... INFO @ Wed, 10 Oct 2018 00:05:49: #1 read treatment tags... INFO @ Wed, 10 Oct 2018 00:05:55: #1 tag size is determined as 101 bps INFO @ Wed, 10 Oct 2018 00:05:55: #1 tag size = 101 INFO @ Wed, 10 Oct 2018 00:05:55: #1 total tags in treatment: 683511 INFO @ Wed, 10 Oct 2018 00:05:55: #1 user defined the maximum tags... INFO @ Wed, 10 Oct 2018 00:05:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 10 Oct 2018 00:05:55: #1 tags after filtering in treatment: 683511 INFO @ Wed, 10 Oct 2018 00:05:55: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 10 Oct 2018 00:05:55: #1 finished! INFO @ Wed, 10 Oct 2018 00:05:55: #2 Build Peak Model... INFO @ Wed, 10 Oct 2018 00:05:55: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 10 Oct 2018 00:05:55: #2 number of paired peaks: 35 WARNING @ Wed, 10 Oct 2018 00:05:55: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 10 Oct 2018 00:05:55: Process for pairing-model is terminated! cat: SRX4733463.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4733463.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4733463.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4733463.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 10 Oct 2018 00:05:55: #1 tag size is determined as 101 bps INFO @ Wed, 10 Oct 2018 00:05:55: #1 tag size = 101 INFO @ Wed, 10 Oct 2018 00:05:55: #1 total tags in treatment: 683511 INFO @ Wed, 10 Oct 2018 00:05:55: #1 user defined the maximum tags... INFO @ Wed, 10 Oct 2018 00:05:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 10 Oct 2018 00:05:55: #1 tags after filtering in treatment: 683511 INFO @ Wed, 10 Oct 2018 00:05:55: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 10 Oct 2018 00:05:55: #1 finished! INFO @ Wed, 10 Oct 2018 00:05:55: #2 Build Peak Model... INFO @ Wed, 10 Oct 2018 00:05:55: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 10 Oct 2018 00:05:55: #1 tag size is determined as 101 bps INFO @ Wed, 10 Oct 2018 00:05:55: #1 tag size = 101 INFO @ Wed, 10 Oct 2018 00:05:55: #1 total tags in treatment: 683511 INFO @ Wed, 10 Oct 2018 00:05:55: #1 user defined the maximum tags... INFO @ Wed, 10 Oct 2018 00:05:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 10 Oct 2018 00:05:55: #1 tags after filtering in treatment: 683511 INFO @ Wed, 10 Oct 2018 00:05:55: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 10 Oct 2018 00:05:55: #1 finished! INFO @ Wed, 10 Oct 2018 00:05:55: #2 Build Peak Model... INFO @ Wed, 10 Oct 2018 00:05:55: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 10 Oct 2018 00:05:55: #2 number of paired peaks: 35 WARNING @ Wed, 10 Oct 2018 00:05:55: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 10 Oct 2018 00:05:55: Process for pairing-model is terminated! cat: SRX4733463.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) INFO @ Wed, 10 Oct 2018 00:05:55: #2 number of paired peaks: 35 WARNING @ Wed, 10 Oct 2018 00:05:55: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 10 Oct 2018 00:05:55: Process for pairing-model is terminated! rm: cannot remove `SRX4733463.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4733463.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4733463.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling cat: SRX4733463.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4733463.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4733463.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4733463.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。