Job ID = 2011729 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 21,365,034 reads read : 21,365,034 reads written : 21,365,034 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:19 21365034 reads; of these: 21365034 (100.00%) were unpaired; of these: 651245 (3.05%) aligned 0 times 17253236 (80.75%) aligned exactly 1 time 3460553 (16.20%) aligned >1 times 96.95% overall alignment rate Time searching: 00:04:19 Overall time: 00:04:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 8960567 / 20713789 = 0.4326 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 02:31:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4726628/SRX4726628.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4726628/SRX4726628.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4726628/SRX4726628.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4726628/SRX4726628.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:31:29: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:31:29: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:31:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4726628/SRX4726628.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4726628/SRX4726628.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4726628/SRX4726628.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4726628/SRX4726628.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:31:30: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:31:30: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:31:36: 1000000 INFO @ Sat, 06 Jul 2019 02:31:38: 1000000 INFO @ Sat, 06 Jul 2019 02:31:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4726628/SRX4726628.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4726628/SRX4726628.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4726628/SRX4726628.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4726628/SRX4726628.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:31:40: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:31:40: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:31:43: 2000000 INFO @ Sat, 06 Jul 2019 02:31:45: 2000000 INFO @ Sat, 06 Jul 2019 02:31:48: 1000000 INFO @ Sat, 06 Jul 2019 02:31:49: 3000000 INFO @ Sat, 06 Jul 2019 02:31:53: 3000000 INFO @ Sat, 06 Jul 2019 02:31:55: 4000000 INFO @ Sat, 06 Jul 2019 02:31:57: 2000000 INFO @ Sat, 06 Jul 2019 02:32:01: 5000000 INFO @ Sat, 06 Jul 2019 02:32:01: 4000000 INFO @ Sat, 06 Jul 2019 02:32:05: 3000000 INFO @ Sat, 06 Jul 2019 02:32:07: 6000000 INFO @ Sat, 06 Jul 2019 02:32:09: 5000000 INFO @ Sat, 06 Jul 2019 02:32:13: 4000000 INFO @ Sat, 06 Jul 2019 02:32:13: 7000000 INFO @ Sat, 06 Jul 2019 02:32:18: 6000000 INFO @ Sat, 06 Jul 2019 02:32:19: 8000000 INFO @ Sat, 06 Jul 2019 02:32:21: 5000000 INFO @ Sat, 06 Jul 2019 02:32:26: 9000000 INFO @ Sat, 06 Jul 2019 02:32:26: 7000000 INFO @ Sat, 06 Jul 2019 02:32:29: 6000000 INFO @ Sat, 06 Jul 2019 02:32:32: 10000000 INFO @ Sat, 06 Jul 2019 02:32:34: 8000000 INFO @ Sat, 06 Jul 2019 02:32:37: 7000000 INFO @ Sat, 06 Jul 2019 02:32:38: 11000000 INFO @ Sat, 06 Jul 2019 02:32:42: 9000000 INFO @ Sat, 06 Jul 2019 02:32:43: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:32:43: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:32:43: #1 total tags in treatment: 11753222 INFO @ Sat, 06 Jul 2019 02:32:43: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:32:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:32:43: #1 tags after filtering in treatment: 11753222 INFO @ Sat, 06 Jul 2019 02:32:43: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:32:43: #1 finished! INFO @ Sat, 06 Jul 2019 02:32:43: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:32:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:32:44: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:32:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:32:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4726628/SRX4726628.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4726628/SRX4726628.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4726628/SRX4726628.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4726628/SRX4726628.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 02:32:45: 8000000 INFO @ Sat, 06 Jul 2019 02:32:50: 10000000 INFO @ Sat, 06 Jul 2019 02:32:53: 9000000 INFO @ Sat, 06 Jul 2019 02:32:58: 11000000 INFO @ Sat, 06 Jul 2019 02:33:01: 10000000 INFO @ Sat, 06 Jul 2019 02:33:04: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:33:04: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:33:04: #1 total tags in treatment: 11753222 INFO @ Sat, 06 Jul 2019 02:33:04: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:33:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:33:05: #1 tags after filtering in treatment: 11753222 INFO @ Sat, 06 Jul 2019 02:33:05: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:33:05: #1 finished! INFO @ Sat, 06 Jul 2019 02:33:05: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:33:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:33:05: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:33:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:33:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4726628/SRX4726628.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4726628/SRX4726628.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4726628/SRX4726628.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4726628/SRX4726628.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 02:33:09: 11000000 INFO @ Sat, 06 Jul 2019 02:33:15: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:33:15: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:33:15: #1 total tags in treatment: 11753222 INFO @ Sat, 06 Jul 2019 02:33:15: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:33:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:33:15: #1 tags after filtering in treatment: 11753222 INFO @ Sat, 06 Jul 2019 02:33:15: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:33:15: #1 finished! INFO @ Sat, 06 Jul 2019 02:33:15: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:33:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:33:16: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:33:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:33:16: Process for pairing-model is terminated! BedGraph に変換しました。 cut: /home/okishinya/chipatlas/results/sacCer3/SRX4726628/SRX4726628.20_peaks.narrowPeak: No such file or directory BigWig に変換中... pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4726628/SRX4726628.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4726628/SRX4726628.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4726628/SRX4726628.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。