Job ID = 2011721 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 22,605,480 reads read : 22,605,480 reads written : 22,605,480 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:05 22605480 reads; of these: 22605480 (100.00%) were unpaired; of these: 4205975 (18.61%) aligned 0 times 15094036 (66.77%) aligned exactly 1 time 3305469 (14.62%) aligned >1 times 81.39% overall alignment rate Time searching: 00:04:05 Overall time: 00:04:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 8713453 / 18399505 = 0.4736 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 02:29:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4726620/SRX4726620.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4726620/SRX4726620.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4726620/SRX4726620.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4726620/SRX4726620.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:29:10: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:29:10: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:29:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4726620/SRX4726620.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4726620/SRX4726620.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4726620/SRX4726620.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4726620/SRX4726620.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:29:11: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:29:11: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:29:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4726620/SRX4726620.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4726620/SRX4726620.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4726620/SRX4726620.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4726620/SRX4726620.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:29:12: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:29:12: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:29:17: 1000000 INFO @ Sat, 06 Jul 2019 02:29:18: 1000000 INFO @ Sat, 06 Jul 2019 02:29:21: 1000000 INFO @ Sat, 06 Jul 2019 02:29:24: 2000000 INFO @ Sat, 06 Jul 2019 02:29:24: 2000000 INFO @ Sat, 06 Jul 2019 02:29:29: 2000000 INFO @ Sat, 06 Jul 2019 02:29:30: 3000000 INFO @ Sat, 06 Jul 2019 02:29:31: 3000000 INFO @ Sat, 06 Jul 2019 02:29:36: 3000000 INFO @ Sat, 06 Jul 2019 02:29:37: 4000000 INFO @ Sat, 06 Jul 2019 02:29:38: 4000000 INFO @ Sat, 06 Jul 2019 02:29:43: 5000000 INFO @ Sat, 06 Jul 2019 02:29:44: 4000000 INFO @ Sat, 06 Jul 2019 02:29:44: 5000000 INFO @ Sat, 06 Jul 2019 02:29:49: 6000000 INFO @ Sat, 06 Jul 2019 02:29:51: 6000000 INFO @ Sat, 06 Jul 2019 02:29:51: 5000000 INFO @ Sat, 06 Jul 2019 02:29:56: 7000000 INFO @ Sat, 06 Jul 2019 02:29:58: 7000000 INFO @ Sat, 06 Jul 2019 02:29:59: 6000000 INFO @ Sat, 06 Jul 2019 02:30:02: 8000000 INFO @ Sat, 06 Jul 2019 02:30:04: 8000000 INFO @ Sat, 06 Jul 2019 02:30:06: 7000000 INFO @ Sat, 06 Jul 2019 02:30:08: 9000000 INFO @ Sat, 06 Jul 2019 02:30:11: 9000000 INFO @ Sat, 06 Jul 2019 02:30:13: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:30:13: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:30:13: #1 total tags in treatment: 9686052 INFO @ Sat, 06 Jul 2019 02:30:13: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:30:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:30:13: #1 tags after filtering in treatment: 9686052 INFO @ Sat, 06 Jul 2019 02:30:13: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:30:13: #1 finished! INFO @ Sat, 06 Jul 2019 02:30:13: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:30:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:30:14: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:30:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:30:14: Process for pairing-model is terminated! INFO @ Sat, 06 Jul 2019 02:30:14: 8000000 INFO @ Sat, 06 Jul 2019 02:30:16: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:30:16: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:30:16: #1 total tags in treatment: 9686052 INFO @ Sat, 06 Jul 2019 02:30:16: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:30:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:30:16: #1 tags after filtering in treatment: 9686052 INFO @ Sat, 06 Jul 2019 02:30:16: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:30:16: #1 finished! INFO @ Sat, 06 Jul 2019 02:30:16: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:30:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:30:17: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:30:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:30:17: Process for pairing-model is terminated! INFO @ Sat, 06 Jul 2019 02:30:21: 9000000 cut: /home/okishinya/chipatlas/results/sacCer3/SRX4726620/SRX4726620.10_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/SRX4726620/SRX4726620.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis pass1 - making usageList (0 chroms)needLargeMem: trying to allocate 0 bytes (limit: 17179869184): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4726620/SRX4726620.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4726620/SRX4726620.10_*.xls’rm: : No such file or directorycannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4726620/SRX4726620.05_model.r’ : No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4726620/SRX4726620.10_peaks.narrowPeak’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4726620/SRX4726620.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4726620/SRX4726620.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 02:30:26: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:30:26: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:30:26: #1 total tags in treatment: 9686052 INFO @ Sat, 06 Jul 2019 02:30:26: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:30:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:30:27: #1 tags after filtering in treatment: 9686052 INFO @ Sat, 06 Jul 2019 02:30:27: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:30:27: #1 finished! INFO @ Sat, 06 Jul 2019 02:30:27: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:30:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:30:27: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:30:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:30:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4726620/SRX4726620.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4726620/SRX4726620.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4726620/SRX4726620.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4726620/SRX4726620.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。