Job ID = 3805973 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 10,770,368 reads read : 10,770,368 reads written : 10,770,368 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:18 10770368 reads; of these: 10770368 (100.00%) were unpaired; of these: 382374 (3.55%) aligned 0 times 8932145 (82.93%) aligned exactly 1 time 1455849 (13.52%) aligned >1 times 96.45% overall alignment rate Time searching: 00:03:18 Overall time: 00:03:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2936858 / 10387994 = 0.2827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Mon, 04 Nov 2019 02:15:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4702301/SRX4702301.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4702301/SRX4702301.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4702301/SRX4702301.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4702301/SRX4702301.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 04 Nov 2019 02:15:53: #1 read tag files... INFO @ Mon, 04 Nov 2019 02:15:53: #1 read treatment tags... INFO @ Mon, 04 Nov 2019 02:16:03: 1000000 INFO @ Mon, 04 Nov 2019 02:16:14: 2000000 INFO @ Mon, 04 Nov 2019 02:16:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4702301/SRX4702301.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4702301/SRX4702301.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4702301/SRX4702301.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4702301/SRX4702301.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 04 Nov 2019 02:16:23: #1 read tag files... INFO @ Mon, 04 Nov 2019 02:16:23: #1 read treatment tags... INFO @ Mon, 04 Nov 2019 02:16:25: 3000000 INFO @ Mon, 04 Nov 2019 02:16:33: 1000000 INFO @ Mon, 04 Nov 2019 02:16:35: 4000000 INFO @ Mon, 04 Nov 2019 02:16:44: 2000000 INFO @ Mon, 04 Nov 2019 02:16:45: 5000000 BedGraph に変換中... INFO @ Mon, 04 Nov 2019 02:16:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4702301/SRX4702301.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4702301/SRX4702301.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4702301/SRX4702301.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4702301/SRX4702301.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 04 Nov 2019 02:16:53: #1 read tag files... INFO @ Mon, 04 Nov 2019 02:16:53: #1 read treatment tags... INFO @ Mon, 04 Nov 2019 02:16:54: 3000000 INFO @ Mon, 04 Nov 2019 02:16:55: 6000000 INFO @ Mon, 04 Nov 2019 02:17:03: 1000000 INFO @ Mon, 04 Nov 2019 02:17:05: 4000000 INFO @ Mon, 04 Nov 2019 02:17:06: 7000000 INFO @ Mon, 04 Nov 2019 02:17:10: #1 tag size is determined as 101 bps INFO @ Mon, 04 Nov 2019 02:17:10: #1 tag size = 101 INFO @ Mon, 04 Nov 2019 02:17:10: #1 total tags in treatment: 7451136 INFO @ Mon, 04 Nov 2019 02:17:10: #1 user defined the maximum tags... INFO @ Mon, 04 Nov 2019 02:17:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 04 Nov 2019 02:17:10: #1 tags after filtering in treatment: 7451136 INFO @ Mon, 04 Nov 2019 02:17:10: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 04 Nov 2019 02:17:10: #1 finished! INFO @ Mon, 04 Nov 2019 02:17:10: #2 Build Peak Model... INFO @ Mon, 04 Nov 2019 02:17:10: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 04 Nov 2019 02:17:11: #2 number of paired peaks: 0 WARNING @ Mon, 04 Nov 2019 02:17:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 04 Nov 2019 02:17:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4702301/SRX4702301.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4702301/SRX4702301.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4702301/SRX4702301.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4702301/SRX4702301.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 04 Nov 2019 02:17:14: 2000000 INFO @ Mon, 04 Nov 2019 02:17:15: 5000000 INFO @ Mon, 04 Nov 2019 02:17:25: 6000000 INFO @ Mon, 04 Nov 2019 02:17:25: 3000000 INFO @ Mon, 04 Nov 2019 02:17:34: 7000000 INFO @ Mon, 04 Nov 2019 02:17:35: 4000000 INFO @ Mon, 04 Nov 2019 02:17:39: #1 tag size is determined as 101 bps INFO @ Mon, 04 Nov 2019 02:17:39: #1 tag size = 101 INFO @ Mon, 04 Nov 2019 02:17:39: #1 total tags in treatment: 7451136 INFO @ Mon, 04 Nov 2019 02:17:39: #1 user defined the maximum tags... INFO @ Mon, 04 Nov 2019 02:17:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 04 Nov 2019 02:17:39: #1 tags after filtering in treatment: 7451136 INFO @ Mon, 04 Nov 2019 02:17:39: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 04 Nov 2019 02:17:39: #1 finished! INFO @ Mon, 04 Nov 2019 02:17:39: #2 Build Peak Model... INFO @ Mon, 04 Nov 2019 02:17:39: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 04 Nov 2019 02:17:40: #2 number of paired peaks: 0 WARNING @ Mon, 04 Nov 2019 02:17:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 04 Nov 2019 02:17:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4702301/SRX4702301.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4702301/SRX4702301.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4702301/SRX4702301.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4702301/SRX4702301.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 04 Nov 2019 02:17:45: 5000000 INFO @ Mon, 04 Nov 2019 02:17:55: 6000000 INFO @ Mon, 04 Nov 2019 02:18:06: 7000000 INFO @ Mon, 04 Nov 2019 02:18:10: #1 tag size is determined as 101 bps INFO @ Mon, 04 Nov 2019 02:18:10: #1 tag size = 101 INFO @ Mon, 04 Nov 2019 02:18:10: #1 total tags in treatment: 7451136 INFO @ Mon, 04 Nov 2019 02:18:10: #1 user defined the maximum tags... INFO @ Mon, 04 Nov 2019 02:18:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 04 Nov 2019 02:18:10: #1 tags after filtering in treatment: 7451136 INFO @ Mon, 04 Nov 2019 02:18:10: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 04 Nov 2019 02:18:10: #1 finished! INFO @ Mon, 04 Nov 2019 02:18:10: #2 Build Peak Model... INFO @ Mon, 04 Nov 2019 02:18:10: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 04 Nov 2019 02:18:11: #2 number of paired peaks: 0 WARNING @ Mon, 04 Nov 2019 02:18:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 04 Nov 2019 02:18:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4702301/SRX4702301.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4702301/SRX4702301.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4702301/SRX4702301.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4702301/SRX4702301.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。