Job ID = 11245196 sra ファイルのダウンロード中... Completed: 412380K bytes transferred in 5 seconds (574547K bits/sec), in 1 file. Completed: 13778K bytes transferred in 2 seconds (39151K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 127694 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4669650/SRR7818265.sra Written 127694 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4669650/SRR7818265.sra Read 4000000 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4669650/SRR7818264.sra Written 4000000 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4669650/SRR7818264.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:51 4127694 reads; of these: 4127694 (100.00%) were paired; of these: 706770 (17.12%) aligned concordantly 0 times 3066181 (74.28%) aligned concordantly exactly 1 time 354743 (8.59%) aligned concordantly >1 times ---- 706770 pairs aligned concordantly 0 times; of these: 49922 (7.06%) aligned discordantly 1 time ---- 656848 pairs aligned 0 times concordantly or discordantly; of these: 1313696 mates make up the pairs; of these: 1182459 (90.01%) aligned 0 times 92019 (7.00%) aligned exactly 1 time 39218 (2.99%) aligned >1 times 85.68% overall alignment rate Time searching: 00:03:51 Overall time: 00:03:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 32570 / 3461025 = 0.0094 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 09 Oct 2018 23:54:01: # Command line: callpeak -t SRX4669650.bam -f BAM -g 12100000 -n SRX4669650.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4669650.20 # format = BAM # ChIP-seq file = ['SRX4669650.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 09 Oct 2018 23:54:01: #1 read tag files... INFO @ Tue, 09 Oct 2018 23:54:01: #1 read treatment tags... INFO @ Tue, 09 Oct 2018 23:54:01: # Command line: callpeak -t SRX4669650.bam -f BAM -g 12100000 -n SRX4669650.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4669650.10 # format = BAM # ChIP-seq file = ['SRX4669650.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 09 Oct 2018 23:54:01: #1 read tag files... INFO @ Tue, 09 Oct 2018 23:54:01: # Command line: callpeak -t SRX4669650.bam -f BAM -g 12100000 -n SRX4669650.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4669650.05 # format = BAM # ChIP-seq file = ['SRX4669650.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 09 Oct 2018 23:54:01: #1 read treatment tags... INFO @ Tue, 09 Oct 2018 23:54:01: #1 read tag files... INFO @ Tue, 09 Oct 2018 23:54:01: #1 read treatment tags... INFO @ Tue, 09 Oct 2018 23:54:07: 1000000 INFO @ Tue, 09 Oct 2018 23:54:08: 1000000 INFO @ Tue, 09 Oct 2018 23:54:08: 1000000 INFO @ Tue, 09 Oct 2018 23:54:14: 2000000 INFO @ Tue, 09 Oct 2018 23:54:14: 2000000 INFO @ Tue, 09 Oct 2018 23:54:14: 2000000 INFO @ Tue, 09 Oct 2018 23:54:20: 3000000 INFO @ Tue, 09 Oct 2018 23:54:21: 3000000 INFO @ Tue, 09 Oct 2018 23:54:21: 3000000 INFO @ Tue, 09 Oct 2018 23:54:27: 4000000 INFO @ Tue, 09 Oct 2018 23:54:28: 4000000 INFO @ Tue, 09 Oct 2018 23:54:28: 4000000 INFO @ Tue, 09 Oct 2018 23:54:33: 5000000 INFO @ Tue, 09 Oct 2018 23:54:35: 5000000 INFO @ Tue, 09 Oct 2018 23:54:35: 5000000 INFO @ Tue, 09 Oct 2018 23:54:39: 6000000 INFO @ Tue, 09 Oct 2018 23:54:42: 6000000 INFO @ Tue, 09 Oct 2018 23:54:42: 6000000 INFO @ Tue, 09 Oct 2018 23:54:46: 7000000 INFO @ Tue, 09 Oct 2018 23:54:46: #1 tag size is determined as 75 bps INFO @ Tue, 09 Oct 2018 23:54:46: #1 tag size = 75 INFO @ Tue, 09 Oct 2018 23:54:46: #1 total tags in treatment: 3388643 INFO @ Tue, 09 Oct 2018 23:54:46: #1 user defined the maximum tags... INFO @ Tue, 09 Oct 2018 23:54:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 09 Oct 2018 23:54:46: #1 tags after filtering in treatment: 2940364 INFO @ Tue, 09 Oct 2018 23:54:46: #1 Redundant rate of treatment: 0.13 INFO @ Tue, 09 Oct 2018 23:54:46: #1 finished! INFO @ Tue, 09 Oct 2018 23:54:46: #2 Build Peak Model... INFO @ Tue, 09 Oct 2018 23:54:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 09 Oct 2018 23:54:46: #2 number of paired peaks: 49 WARNING @ Tue, 09 Oct 2018 23:54:46: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 09 Oct 2018 23:54:46: Process for pairing-model is terminated! cat: SRX4669650.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4669650.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669650.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669650.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 09 Oct 2018 23:54:49: 7000000 INFO @ Tue, 09 Oct 2018 23:54:49: 7000000 INFO @ Tue, 09 Oct 2018 23:54:49: #1 tag size is determined as 75 bps INFO @ Tue, 09 Oct 2018 23:54:49: #1 tag size = 75 INFO @ Tue, 09 Oct 2018 23:54:49: #1 total tags in treatment: 3388643 INFO @ Tue, 09 Oct 2018 23:54:49: #1 user defined the maximum tags... INFO @ Tue, 09 Oct 2018 23:54:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 09 Oct 2018 23:54:49: #1 tag size is determined as 75 bps INFO @ Tue, 09 Oct 2018 23:54:49: #1 tag size = 75 INFO @ Tue, 09 Oct 2018 23:54:49: #1 total tags in treatment: 3388643 INFO @ Tue, 09 Oct 2018 23:54:49: #1 user defined the maximum tags... INFO @ Tue, 09 Oct 2018 23:54:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 09 Oct 2018 23:54:49: #1 tags after filtering in treatment: 2940364 INFO @ Tue, 09 Oct 2018 23:54:49: #1 Redundant rate of treatment: 0.13 INFO @ Tue, 09 Oct 2018 23:54:49: #1 finished! INFO @ Tue, 09 Oct 2018 23:54:49: #2 Build Peak Model... INFO @ Tue, 09 Oct 2018 23:54:49: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 09 Oct 2018 23:54:49: #1 tags after filtering in treatment: 2940364 INFO @ Tue, 09 Oct 2018 23:54:49: #1 Redundant rate of treatment: 0.13 INFO @ Tue, 09 Oct 2018 23:54:49: #1 finished! INFO @ Tue, 09 Oct 2018 23:54:49: #2 Build Peak Model... INFO @ Tue, 09 Oct 2018 23:54:49: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 09 Oct 2018 23:54:50: #2 number of paired peaks: 49 WARNING @ Tue, 09 Oct 2018 23:54:50: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 09 Oct 2018 23:54:50: Process for pairing-model is terminated! INFO @ Tue, 09 Oct 2018 23:54:50: #2 number of paired peaks: 49 WARNING @ Tue, 09 Oct 2018 23:54:50: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 09 Oct 2018 23:54:50: Process for pairing-model is terminated! cat: SRX4669650.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX4669650.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4669650.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669650.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669650.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4669650.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669650.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669650.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。