Job ID = 11245159 sra ファイルのダウンロード中... Completed: 337539K bytes transferred in 6 seconds (415056K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 6973093 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4669608/SRR7818191.sra Written 6973093 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4669608/SRR7818191.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:50 6973093 reads; of these: 6973093 (100.00%) were paired; of these: 716165 (10.27%) aligned concordantly 0 times 5412962 (77.63%) aligned concordantly exactly 1 time 843966 (12.10%) aligned concordantly >1 times ---- 716165 pairs aligned concordantly 0 times; of these: 32456 (4.53%) aligned discordantly 1 time ---- 683709 pairs aligned 0 times concordantly or discordantly; of these: 1367418 mates make up the pairs; of these: 1110617 (81.22%) aligned 0 times 175681 (12.85%) aligned exactly 1 time 81120 (5.93%) aligned >1 times 92.04% overall alignment rate Time searching: 00:04:50 Overall time: 00:04:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 96847 / 6272839 = 0.0154 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 09 Oct 2018 23:47:32: # Command line: callpeak -t SRX4669608.bam -f BAM -g 12100000 -n SRX4669608.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4669608.05 # format = BAM # ChIP-seq file = ['SRX4669608.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 09 Oct 2018 23:47:32: #1 read tag files... INFO @ Tue, 09 Oct 2018 23:47:32: #1 read treatment tags... INFO @ Tue, 09 Oct 2018 23:47:32: # Command line: callpeak -t SRX4669608.bam -f BAM -g 12100000 -n SRX4669608.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4669608.10 # format = BAM # ChIP-seq file = ['SRX4669608.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 09 Oct 2018 23:47:32: #1 read tag files... INFO @ Tue, 09 Oct 2018 23:47:32: #1 read treatment tags... INFO @ Tue, 09 Oct 2018 23:47:32: # Command line: callpeak -t SRX4669608.bam -f BAM -g 12100000 -n SRX4669608.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4669608.20 # format = BAM # ChIP-seq file = ['SRX4669608.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 09 Oct 2018 23:47:32: #1 read tag files... INFO @ Tue, 09 Oct 2018 23:47:32: #1 read treatment tags... INFO @ Tue, 09 Oct 2018 23:47:37: 1000000 INFO @ Tue, 09 Oct 2018 23:47:37: 1000000 INFO @ Tue, 09 Oct 2018 23:47:37: 1000000 INFO @ Tue, 09 Oct 2018 23:47:43: 2000000 INFO @ Tue, 09 Oct 2018 23:47:43: 2000000 INFO @ Tue, 09 Oct 2018 23:47:43: 2000000 INFO @ Tue, 09 Oct 2018 23:47:49: 3000000 INFO @ Tue, 09 Oct 2018 23:47:49: 3000000 INFO @ Tue, 09 Oct 2018 23:47:49: 3000000 INFO @ Tue, 09 Oct 2018 23:47:55: 4000000 INFO @ Tue, 09 Oct 2018 23:47:55: 4000000 INFO @ Tue, 09 Oct 2018 23:47:55: 4000000 INFO @ Tue, 09 Oct 2018 23:48:00: 5000000 INFO @ Tue, 09 Oct 2018 23:48:00: 5000000 INFO @ Tue, 09 Oct 2018 23:48:01: 5000000 INFO @ Tue, 09 Oct 2018 23:48:06: 6000000 INFO @ Tue, 09 Oct 2018 23:48:06: 6000000 INFO @ Tue, 09 Oct 2018 23:48:07: 6000000 INFO @ Tue, 09 Oct 2018 23:48:12: 7000000 INFO @ Tue, 09 Oct 2018 23:48:12: 7000000 INFO @ Tue, 09 Oct 2018 23:48:13: 7000000 INFO @ Tue, 09 Oct 2018 23:48:18: 8000000 INFO @ Tue, 09 Oct 2018 23:48:18: 8000000 INFO @ Tue, 09 Oct 2018 23:48:18: 8000000 INFO @ Tue, 09 Oct 2018 23:48:23: 9000000 INFO @ Tue, 09 Oct 2018 23:48:24: 9000000 INFO @ Tue, 09 Oct 2018 23:48:24: 9000000 INFO @ Tue, 09 Oct 2018 23:48:29: 10000000 INFO @ Tue, 09 Oct 2018 23:48:30: 10000000 INFO @ Tue, 09 Oct 2018 23:48:30: 10000000 INFO @ Tue, 09 Oct 2018 23:48:35: 11000000 INFO @ Tue, 09 Oct 2018 23:48:35: 11000000 INFO @ Tue, 09 Oct 2018 23:48:36: 11000000 INFO @ Tue, 09 Oct 2018 23:48:41: 12000000 INFO @ Tue, 09 Oct 2018 23:48:41: 12000000 INFO @ Tue, 09 Oct 2018 23:48:41: 12000000 INFO @ Tue, 09 Oct 2018 23:48:44: #1 tag size is determined as 50 bps INFO @ Tue, 09 Oct 2018 23:48:44: #1 tag size = 50 INFO @ Tue, 09 Oct 2018 23:48:44: #1 total tags in treatment: 6160420 INFO @ Tue, 09 Oct 2018 23:48:44: #1 user defined the maximum tags... INFO @ Tue, 09 Oct 2018 23:48:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 09 Oct 2018 23:48:44: #1 tags after filtering in treatment: 4960028 INFO @ Tue, 09 Oct 2018 23:48:44: #1 Redundant rate of treatment: 0.19 INFO @ Tue, 09 Oct 2018 23:48:44: #1 finished! INFO @ Tue, 09 Oct 2018 23:48:44: #2 Build Peak Model... INFO @ Tue, 09 Oct 2018 23:48:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 09 Oct 2018 23:48:45: #2 number of paired peaks: 0 WARNING @ Tue, 09 Oct 2018 23:48:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 09 Oct 2018 23:48:45: Process for pairing-model is terminated! cat: SRX4669608.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4669608.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669608.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669608.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 09 Oct 2018 23:48:45: #1 tag size is determined as 50 bps INFO @ Tue, 09 Oct 2018 23:48:45: #1 tag size = 50 INFO @ Tue, 09 Oct 2018 23:48:45: #1 total tags in treatment: 6160420 INFO @ Tue, 09 Oct 2018 23:48:45: #1 user defined the maximum tags... INFO @ Tue, 09 Oct 2018 23:48:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 09 Oct 2018 23:48:45: #1 tags after filtering in treatment: 4960028 INFO @ Tue, 09 Oct 2018 23:48:45: #1 Redundant rate of treatment: 0.19 INFO @ Tue, 09 Oct 2018 23:48:45: #1 finished! INFO @ Tue, 09 Oct 2018 23:48:45: #2 Build Peak Model... INFO @ Tue, 09 Oct 2018 23:48:45: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 09 Oct 2018 23:48:45: #1 tag size is determined as 50 bps INFO @ Tue, 09 Oct 2018 23:48:45: #1 tag size = 50 INFO @ Tue, 09 Oct 2018 23:48:45: #1 total tags in treatment: 6160420 INFO @ Tue, 09 Oct 2018 23:48:45: #1 user defined the maximum tags... INFO @ Tue, 09 Oct 2018 23:48:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 09 Oct 2018 23:48:45: #2 number of paired peaks: 0 WARNING @ Tue, 09 Oct 2018 23:48:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 09 Oct 2018 23:48:45: Process for pairing-model is terminated! INFO @ Tue, 09 Oct 2018 23:48:45: #1 tags after filtering in treatment: 4960028 INFO @ Tue, 09 Oct 2018 23:48:45: #1 Redundant rate of treatment: 0.19 INFO @ Tue, 09 Oct 2018 23:48:45: #1 finished! INFO @ Tue, 09 Oct 2018 23:48:45: #2 Build Peak Model... INFO @ Tue, 09 Oct 2018 23:48:45: #2 looking for paired plus/minus strand peaks... cat: SRX4669608.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4669608.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669608.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669608.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 09 Oct 2018 23:48:46: #2 number of paired peaks: 0 WARNING @ Tue, 09 Oct 2018 23:48:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 09 Oct 2018 23:48:46: Process for pairing-model is terminated! cat: SRX4669608.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4669608.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669608.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669608.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。