Job ID = 11245155 sra ファイルのダウンロード中... Completed: 385738K bytes transferred in 6 seconds (479034K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 7798787 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4669605/SRR7818188.sra Written 7798787 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4669605/SRR7818188.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:45 7798787 reads; of these: 7798787 (100.00%) were paired; of these: 963198 (12.35%) aligned concordantly 0 times 5893241 (75.57%) aligned concordantly exactly 1 time 942348 (12.08%) aligned concordantly >1 times ---- 963198 pairs aligned concordantly 0 times; of these: 41885 (4.35%) aligned discordantly 1 time ---- 921313 pairs aligned 0 times concordantly or discordantly; of these: 1842626 mates make up the pairs; of these: 1540677 (83.61%) aligned 0 times 207984 (11.29%) aligned exactly 1 time 93965 (5.10%) aligned >1 times 90.12% overall alignment rate Time searching: 00:05:45 Overall time: 00:05:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 103625 / 6851273 = 0.0151 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 09 Oct 2018 23:45:57: # Command line: callpeak -t SRX4669605.bam -f BAM -g 12100000 -n SRX4669605.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4669605.10 # format = BAM # ChIP-seq file = ['SRX4669605.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 09 Oct 2018 23:45:57: #1 read tag files... INFO @ Tue, 09 Oct 2018 23:45:57: #1 read treatment tags... INFO @ Tue, 09 Oct 2018 23:45:57: # Command line: callpeak -t SRX4669605.bam -f BAM -g 12100000 -n SRX4669605.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4669605.05 # format = BAM # ChIP-seq file = ['SRX4669605.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 09 Oct 2018 23:45:57: #1 read tag files... INFO @ Tue, 09 Oct 2018 23:45:57: #1 read treatment tags... INFO @ Tue, 09 Oct 2018 23:45:57: # Command line: callpeak -t SRX4669605.bam -f BAM -g 12100000 -n SRX4669605.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4669605.20 # format = BAM # ChIP-seq file = ['SRX4669605.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 09 Oct 2018 23:45:57: #1 read tag files... INFO @ Tue, 09 Oct 2018 23:45:57: #1 read treatment tags... INFO @ Tue, 09 Oct 2018 23:46:03: 1000000 INFO @ Tue, 09 Oct 2018 23:46:04: 1000000 INFO @ Tue, 09 Oct 2018 23:46:04: 1000000 INFO @ Tue, 09 Oct 2018 23:46:09: 2000000 INFO @ Tue, 09 Oct 2018 23:46:11: 2000000 INFO @ Tue, 09 Oct 2018 23:46:11: 2000000 INFO @ Tue, 09 Oct 2018 23:46:15: 3000000 INFO @ Tue, 09 Oct 2018 23:46:18: 3000000 INFO @ Tue, 09 Oct 2018 23:46:18: 3000000 INFO @ Tue, 09 Oct 2018 23:46:20: 4000000 INFO @ Tue, 09 Oct 2018 23:46:25: 4000000 INFO @ Tue, 09 Oct 2018 23:46:25: 4000000 INFO @ Tue, 09 Oct 2018 23:46:25: 5000000 INFO @ Tue, 09 Oct 2018 23:46:31: 6000000 INFO @ Tue, 09 Oct 2018 23:46:32: 5000000 INFO @ Tue, 09 Oct 2018 23:46:32: 5000000 INFO @ Tue, 09 Oct 2018 23:46:36: 7000000 INFO @ Tue, 09 Oct 2018 23:46:40: 6000000 INFO @ Tue, 09 Oct 2018 23:46:40: 6000000 INFO @ Tue, 09 Oct 2018 23:46:42: 8000000 INFO @ Tue, 09 Oct 2018 23:46:47: 7000000 INFO @ Tue, 09 Oct 2018 23:46:47: 7000000 INFO @ Tue, 09 Oct 2018 23:46:47: 9000000 INFO @ Tue, 09 Oct 2018 23:46:52: 10000000 INFO @ Tue, 09 Oct 2018 23:46:54: 8000000 INFO @ Tue, 09 Oct 2018 23:46:54: 8000000 INFO @ Tue, 09 Oct 2018 23:46:58: 11000000 INFO @ Tue, 09 Oct 2018 23:47:01: 9000000 INFO @ Tue, 09 Oct 2018 23:47:01: 9000000 INFO @ Tue, 09 Oct 2018 23:47:03: 12000000 INFO @ Tue, 09 Oct 2018 23:47:08: 10000000 INFO @ Tue, 09 Oct 2018 23:47:08: 10000000 INFO @ Tue, 09 Oct 2018 23:47:09: 13000000 INFO @ Tue, 09 Oct 2018 23:47:13: #1 tag size is determined as 50 bps INFO @ Tue, 09 Oct 2018 23:47:13: #1 tag size = 50 INFO @ Tue, 09 Oct 2018 23:47:13: #1 total tags in treatment: 6732287 INFO @ Tue, 09 Oct 2018 23:47:13: #1 user defined the maximum tags... INFO @ Tue, 09 Oct 2018 23:47:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 09 Oct 2018 23:47:13: #1 tags after filtering in treatment: 5360208 INFO @ Tue, 09 Oct 2018 23:47:13: #1 Redundant rate of treatment: 0.20 INFO @ Tue, 09 Oct 2018 23:47:13: #1 finished! INFO @ Tue, 09 Oct 2018 23:47:13: #2 Build Peak Model... INFO @ Tue, 09 Oct 2018 23:47:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 09 Oct 2018 23:47:14: #2 number of paired peaks: 0 WARNING @ Tue, 09 Oct 2018 23:47:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 09 Oct 2018 23:47:14: Process for pairing-model is terminated! cat: SRX4669605.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4669605.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669605.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669605.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 09 Oct 2018 23:47:15: 11000000 INFO @ Tue, 09 Oct 2018 23:47:15: 11000000 INFO @ Tue, 09 Oct 2018 23:47:22: 12000000 INFO @ Tue, 09 Oct 2018 23:47:22: 12000000 INFO @ Tue, 09 Oct 2018 23:47:30: 13000000 INFO @ Tue, 09 Oct 2018 23:47:30: 13000000 INFO @ Tue, 09 Oct 2018 23:47:36: #1 tag size is determined as 50 bps INFO @ Tue, 09 Oct 2018 23:47:36: #1 tag size is determined as 50 bps INFO @ Tue, 09 Oct 2018 23:47:36: #1 tag size = 50 INFO @ Tue, 09 Oct 2018 23:47:36: #1 tag size = 50 INFO @ Tue, 09 Oct 2018 23:47:36: #1 total tags in treatment: 6732287 INFO @ Tue, 09 Oct 2018 23:47:36: #1 total tags in treatment: 6732287 INFO @ Tue, 09 Oct 2018 23:47:36: #1 user defined the maximum tags... INFO @ Tue, 09 Oct 2018 23:47:36: #1 user defined the maximum tags... INFO @ Tue, 09 Oct 2018 23:47:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 09 Oct 2018 23:47:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 09 Oct 2018 23:47:36: #1 tags after filtering in treatment: 5360208 INFO @ Tue, 09 Oct 2018 23:47:36: #1 tags after filtering in treatment: 5360208 INFO @ Tue, 09 Oct 2018 23:47:36: #1 Redundant rate of treatment: 0.20 INFO @ Tue, 09 Oct 2018 23:47:36: #1 Redundant rate of treatment: 0.20 INFO @ Tue, 09 Oct 2018 23:47:36: #1 finished! INFO @ Tue, 09 Oct 2018 23:47:36: #1 finished! INFO @ Tue, 09 Oct 2018 23:47:36: #2 Build Peak Model... INFO @ Tue, 09 Oct 2018 23:47:36: #2 Build Peak Model... INFO @ Tue, 09 Oct 2018 23:47:36: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 09 Oct 2018 23:47:36: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 09 Oct 2018 23:47:36: #2 number of paired peaks: 0 WARNING @ Tue, 09 Oct 2018 23:47:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 09 Oct 2018 23:47:36: Process for pairing-model is terminated! INFO @ Tue, 09 Oct 2018 23:47:36: #2 number of paired peaks: 0 WARNING @ Tue, 09 Oct 2018 23:47:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 09 Oct 2018 23:47:36: Process for pairing-model is terminated! cat: SRX4669605.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX4669605.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 1 millis rm: cannot remove `SRX4669605.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669605.20_*.xls': そのようなファイルやディレクトリはありません needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4669605.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling rm: cannot remove `SRX4669605.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669605.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669605.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。