Job ID = 14521885 SRX = SRX4623316 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 7122020 spots for SRR7767721/SRR7767721.sra Written 7122020 spots for SRR7767721/SRR7767721.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:37 7122020 reads; of these: 7122020 (100.00%) were paired; of these: 363948 (5.11%) aligned concordantly 0 times 5880357 (82.57%) aligned concordantly exactly 1 time 877715 (12.32%) aligned concordantly >1 times ---- 363948 pairs aligned concordantly 0 times; of these: 12348 (3.39%) aligned discordantly 1 time ---- 351600 pairs aligned 0 times concordantly or discordantly; of these: 703200 mates make up the pairs; of these: 616823 (87.72%) aligned 0 times 66624 (9.47%) aligned exactly 1 time 19753 (2.81%) aligned >1 times 95.67% overall alignment rate Time searching: 00:02:37 Overall time: 00:02:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 133378 / 6762655 = 0.0197 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:51:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:51:43: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:51:43: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:51:48: 1000000 INFO @ Sat, 15 Jan 2022 21:51:53: 2000000 INFO @ Sat, 15 Jan 2022 21:51:57: 3000000 INFO @ Sat, 15 Jan 2022 21:52:02: 4000000 INFO @ Sat, 15 Jan 2022 21:52:06: 5000000 INFO @ Sat, 15 Jan 2022 21:52:11: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:52:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:52:13: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:52:13: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:52:15: 7000000 INFO @ Sat, 15 Jan 2022 21:52:18: 1000000 INFO @ Sat, 15 Jan 2022 21:52:20: 8000000 INFO @ Sat, 15 Jan 2022 21:52:23: 2000000 INFO @ Sat, 15 Jan 2022 21:52:25: 9000000 INFO @ Sat, 15 Jan 2022 21:52:28: 3000000 INFO @ Sat, 15 Jan 2022 21:52:30: 10000000 INFO @ Sat, 15 Jan 2022 21:52:33: 4000000 INFO @ Sat, 15 Jan 2022 21:52:35: 11000000 INFO @ Sat, 15 Jan 2022 21:52:38: 5000000 INFO @ Sat, 15 Jan 2022 21:52:40: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:52:43: 6000000 INFO @ Sat, 15 Jan 2022 21:52:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:52:43: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:52:43: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:52:44: 13000000 INFO @ Sat, 15 Jan 2022 21:52:46: #1 tag size is determined as 25 bps INFO @ Sat, 15 Jan 2022 21:52:46: #1 tag size = 25 INFO @ Sat, 15 Jan 2022 21:52:46: #1 total tags in treatment: 6624738 INFO @ Sat, 15 Jan 2022 21:52:46: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:52:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:52:46: #1 tags after filtering in treatment: 5358506 INFO @ Sat, 15 Jan 2022 21:52:46: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 15 Jan 2022 21:52:46: #1 finished! INFO @ Sat, 15 Jan 2022 21:52:46: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:52:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:52:46: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:52:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:52:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:52:48: 7000000 INFO @ Sat, 15 Jan 2022 21:52:48: 1000000 INFO @ Sat, 15 Jan 2022 21:52:52: 2000000 INFO @ Sat, 15 Jan 2022 21:52:53: 8000000 INFO @ Sat, 15 Jan 2022 21:52:57: 3000000 INFO @ Sat, 15 Jan 2022 21:52:58: 9000000 INFO @ Sat, 15 Jan 2022 21:53:01: 4000000 INFO @ Sat, 15 Jan 2022 21:53:03: 10000000 INFO @ Sat, 15 Jan 2022 21:53:05: 5000000 INFO @ Sat, 15 Jan 2022 21:53:08: 11000000 INFO @ Sat, 15 Jan 2022 21:53:09: 6000000 INFO @ Sat, 15 Jan 2022 21:53:13: 12000000 INFO @ Sat, 15 Jan 2022 21:53:14: 7000000 INFO @ Sat, 15 Jan 2022 21:53:18: 13000000 INFO @ Sat, 15 Jan 2022 21:53:18: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:53:19: #1 tag size is determined as 25 bps INFO @ Sat, 15 Jan 2022 21:53:19: #1 tag size = 25 INFO @ Sat, 15 Jan 2022 21:53:19: #1 total tags in treatment: 6624738 INFO @ Sat, 15 Jan 2022 21:53:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:53:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:53:20: #1 tags after filtering in treatment: 5358506 INFO @ Sat, 15 Jan 2022 21:53:20: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 15 Jan 2022 21:53:20: #1 finished! INFO @ Sat, 15 Jan 2022 21:53:20: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:53:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:53:20: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:53:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:53:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:53:22: 9000000 INFO @ Sat, 15 Jan 2022 21:53:26: 10000000 INFO @ Sat, 15 Jan 2022 21:53:30: 11000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:53:34: 12000000 INFO @ Sat, 15 Jan 2022 21:53:38: 13000000 INFO @ Sat, 15 Jan 2022 21:53:40: #1 tag size is determined as 25 bps INFO @ Sat, 15 Jan 2022 21:53:40: #1 tag size = 25 INFO @ Sat, 15 Jan 2022 21:53:40: #1 total tags in treatment: 6624738 INFO @ Sat, 15 Jan 2022 21:53:40: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:53:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:53:40: #1 tags after filtering in treatment: 5358506 INFO @ Sat, 15 Jan 2022 21:53:40: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 15 Jan 2022 21:53:40: #1 finished! INFO @ Sat, 15 Jan 2022 21:53:40: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:53:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:53:40: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:53:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:53:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling