Job ID = 10223926 SRX = SRX4623316 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 7122020 spots for SRR7767721/SRR7767721.sra Written 7122020 spots for SRR7767721/SRR7767721.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:33 7122020 reads; of these: 7122020 (100.00%) were paired; of these: 363948 (5.11%) aligned concordantly 0 times 5880357 (82.57%) aligned concordantly exactly 1 time 877715 (12.32%) aligned concordantly >1 times ---- 363948 pairs aligned concordantly 0 times; of these: 12348 (3.39%) aligned discordantly 1 time ---- 351600 pairs aligned 0 times concordantly or discordantly; of these: 703200 mates make up the pairs; of these: 616823 (87.72%) aligned 0 times 66624 (9.47%) aligned exactly 1 time 19753 (2.81%) aligned >1 times 95.67% overall alignment rate Time searching: 00:02:33 Overall time: 00:02:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 133378 / 6762655 = 0.0197 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:54:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:54:54: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:54:54: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:54:58: 1000000 INFO @ Fri, 16 Oct 2020 08:55:03: 2000000 INFO @ Fri, 16 Oct 2020 08:55:07: 3000000 INFO @ Fri, 16 Oct 2020 08:55:11: 4000000 INFO @ Fri, 16 Oct 2020 08:55:16: 5000000 INFO @ Fri, 16 Oct 2020 08:55:20: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:55:24: 7000000 INFO @ Fri, 16 Oct 2020 08:55:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:55:24: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:55:24: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:55:28: 8000000 INFO @ Fri, 16 Oct 2020 08:55:29: 1000000 INFO @ Fri, 16 Oct 2020 08:55:33: 9000000 INFO @ Fri, 16 Oct 2020 08:55:33: 2000000 INFO @ Fri, 16 Oct 2020 08:55:37: 10000000 INFO @ Fri, 16 Oct 2020 08:55:37: 3000000 INFO @ Fri, 16 Oct 2020 08:55:42: 4000000 INFO @ Fri, 16 Oct 2020 08:55:42: 11000000 INFO @ Fri, 16 Oct 2020 08:55:46: 12000000 INFO @ Fri, 16 Oct 2020 08:55:46: 5000000 INFO @ Fri, 16 Oct 2020 08:55:50: 13000000 INFO @ Fri, 16 Oct 2020 08:55:50: 6000000 INFO @ Fri, 16 Oct 2020 08:55:52: #1 tag size is determined as 25 bps INFO @ Fri, 16 Oct 2020 08:55:52: #1 tag size = 25 INFO @ Fri, 16 Oct 2020 08:55:52: #1 total tags in treatment: 6624738 INFO @ Fri, 16 Oct 2020 08:55:52: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 08:55:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 08:55:52: #1 tags after filtering in treatment: 5358506 INFO @ Fri, 16 Oct 2020 08:55:52: #1 Redundant rate of treatment: 0.19 INFO @ Fri, 16 Oct 2020 08:55:52: #1 finished! INFO @ Fri, 16 Oct 2020 08:55:52: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 08:55:52: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 08:55:52: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 08:55:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 08:55:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:55:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:55:54: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:55:54: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:55:54: 7000000 INFO @ Fri, 16 Oct 2020 08:55:59: 8000000 INFO @ Fri, 16 Oct 2020 08:55:59: 1000000 INFO @ Fri, 16 Oct 2020 08:56:03: 9000000 INFO @ Fri, 16 Oct 2020 08:56:04: 2000000 INFO @ Fri, 16 Oct 2020 08:56:08: 10000000 INFO @ Fri, 16 Oct 2020 08:56:09: 3000000 INFO @ Fri, 16 Oct 2020 08:56:12: 11000000 INFO @ Fri, 16 Oct 2020 08:56:14: 4000000 INFO @ Fri, 16 Oct 2020 08:56:16: 12000000 INFO @ Fri, 16 Oct 2020 08:56:19: 5000000 INFO @ Fri, 16 Oct 2020 08:56:21: 13000000 INFO @ Fri, 16 Oct 2020 08:56:22: #1 tag size is determined as 25 bps INFO @ Fri, 16 Oct 2020 08:56:22: #1 tag size = 25 INFO @ Fri, 16 Oct 2020 08:56:22: #1 total tags in treatment: 6624738 INFO @ Fri, 16 Oct 2020 08:56:22: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 08:56:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 08:56:22: #1 tags after filtering in treatment: 5358506 INFO @ Fri, 16 Oct 2020 08:56:22: #1 Redundant rate of treatment: 0.19 INFO @ Fri, 16 Oct 2020 08:56:22: #1 finished! INFO @ Fri, 16 Oct 2020 08:56:22: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 08:56:22: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 08:56:23: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 08:56:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 08:56:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 16 Oct 2020 08:56:23: 6000000 INFO @ Fri, 16 Oct 2020 08:56:28: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 16 Oct 2020 08:56:33: 8000000 INFO @ Fri, 16 Oct 2020 08:56:37: 9000000 BigWig に変換しました。 INFO @ Fri, 16 Oct 2020 08:56:42: 10000000 INFO @ Fri, 16 Oct 2020 08:56:46: 11000000 INFO @ Fri, 16 Oct 2020 08:56:51: 12000000 INFO @ Fri, 16 Oct 2020 08:56:56: 13000000 INFO @ Fri, 16 Oct 2020 08:56:57: #1 tag size is determined as 25 bps INFO @ Fri, 16 Oct 2020 08:56:57: #1 tag size = 25 INFO @ Fri, 16 Oct 2020 08:56:57: #1 total tags in treatment: 6624738 INFO @ Fri, 16 Oct 2020 08:56:57: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 08:56:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 08:56:57: #1 tags after filtering in treatment: 5358506 INFO @ Fri, 16 Oct 2020 08:56:57: #1 Redundant rate of treatment: 0.19 INFO @ Fri, 16 Oct 2020 08:56:57: #1 finished! INFO @ Fri, 16 Oct 2020 08:56:57: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 08:56:57: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 08:56:58: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 08:56:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 08:56:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623316/SRX4623316.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling