Job ID = 14521883 SRX = SRX4623314 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 8563106 spots for SRR7767719/SRR7767719.sra Written 8563106 spots for SRR7767719/SRR7767719.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:13 8563106 reads; of these: 8563106 (100.00%) were paired; of these: 666337 (7.78%) aligned concordantly 0 times 6732075 (78.62%) aligned concordantly exactly 1 time 1164694 (13.60%) aligned concordantly >1 times ---- 666337 pairs aligned concordantly 0 times; of these: 148197 (22.24%) aligned discordantly 1 time ---- 518140 pairs aligned 0 times concordantly or discordantly; of these: 1036280 mates make up the pairs; of these: 773935 (74.68%) aligned 0 times 170529 (16.46%) aligned exactly 1 time 91816 (8.86%) aligned >1 times 95.48% overall alignment rate Time searching: 00:03:13 Overall time: 00:03:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 177024 / 7973229 = 0.0222 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:52:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4623314/SRX4623314.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4623314/SRX4623314.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4623314/SRX4623314.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4623314/SRX4623314.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:52:39: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:52:39: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:52:44: 1000000 INFO @ Sat, 15 Jan 2022 21:52:49: 2000000 INFO @ Sat, 15 Jan 2022 21:52:53: 3000000 INFO @ Sat, 15 Jan 2022 21:52:58: 4000000 INFO @ Sat, 15 Jan 2022 21:53:02: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:53:07: 6000000 INFO @ Sat, 15 Jan 2022 21:53:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4623314/SRX4623314.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4623314/SRX4623314.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4623314/SRX4623314.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4623314/SRX4623314.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:53:09: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:53:09: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:53:13: 7000000 INFO @ Sat, 15 Jan 2022 21:53:15: 1000000 INFO @ Sat, 15 Jan 2022 21:53:19: 8000000 INFO @ Sat, 15 Jan 2022 21:53:21: 2000000 INFO @ Sat, 15 Jan 2022 21:53:25: 9000000 INFO @ Sat, 15 Jan 2022 21:53:28: 3000000 INFO @ Sat, 15 Jan 2022 21:53:31: 10000000 INFO @ Sat, 15 Jan 2022 21:53:35: 4000000 INFO @ Sat, 15 Jan 2022 21:53:37: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:53:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4623314/SRX4623314.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4623314/SRX4623314.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4623314/SRX4623314.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4623314/SRX4623314.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:53:39: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:53:39: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:53:42: 5000000 INFO @ Sat, 15 Jan 2022 21:53:43: 12000000 INFO @ Sat, 15 Jan 2022 21:53:45: 1000000 INFO @ Sat, 15 Jan 2022 21:53:49: 13000000 INFO @ Sat, 15 Jan 2022 21:53:49: 6000000 INFO @ Sat, 15 Jan 2022 21:53:52: 2000000 INFO @ Sat, 15 Jan 2022 21:53:55: 14000000 INFO @ Sat, 15 Jan 2022 21:53:55: 7000000 INFO @ Sat, 15 Jan 2022 21:53:58: 3000000 INFO @ Sat, 15 Jan 2022 21:54:01: 15000000 INFO @ Sat, 15 Jan 2022 21:54:02: 8000000 INFO @ Sat, 15 Jan 2022 21:54:04: 4000000 INFO @ Sat, 15 Jan 2022 21:54:07: #1 tag size is determined as 25 bps INFO @ Sat, 15 Jan 2022 21:54:07: #1 tag size = 25 INFO @ Sat, 15 Jan 2022 21:54:07: #1 total tags in treatment: 7720187 INFO @ Sat, 15 Jan 2022 21:54:07: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:54:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:54:07: #1 tags after filtering in treatment: 5885965 INFO @ Sat, 15 Jan 2022 21:54:07: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 21:54:07: #1 finished! INFO @ Sat, 15 Jan 2022 21:54:07: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:54:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:54:07: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:54:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:54:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4623314/SRX4623314.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623314/SRX4623314.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623314/SRX4623314.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623314/SRX4623314.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:54:09: 9000000 INFO @ Sat, 15 Jan 2022 21:54:10: 5000000 INFO @ Sat, 15 Jan 2022 21:54:16: 6000000 INFO @ Sat, 15 Jan 2022 21:54:16: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:54:22: 7000000 INFO @ Sat, 15 Jan 2022 21:54:23: 11000000 INFO @ Sat, 15 Jan 2022 21:54:28: 8000000 INFO @ Sat, 15 Jan 2022 21:54:30: 12000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:54:34: 9000000 INFO @ Sat, 15 Jan 2022 21:54:37: 13000000 INFO @ Sat, 15 Jan 2022 21:54:40: 10000000 INFO @ Sat, 15 Jan 2022 21:54:44: 14000000 INFO @ Sat, 15 Jan 2022 21:54:46: 11000000 INFO @ Sat, 15 Jan 2022 21:54:51: 15000000 INFO @ Sat, 15 Jan 2022 21:54:52: 12000000 INFO @ Sat, 15 Jan 2022 21:54:58: #1 tag size is determined as 25 bps INFO @ Sat, 15 Jan 2022 21:54:58: #1 tag size = 25 INFO @ Sat, 15 Jan 2022 21:54:58: #1 total tags in treatment: 7720187 INFO @ Sat, 15 Jan 2022 21:54:58: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:54:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:54:58: #1 tags after filtering in treatment: 5885965 INFO @ Sat, 15 Jan 2022 21:54:58: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 21:54:58: #1 finished! INFO @ Sat, 15 Jan 2022 21:54:58: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:54:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:54:58: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:54:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:54:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4623314/SRX4623314.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623314/SRX4623314.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623314/SRX4623314.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623314/SRX4623314.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:54:58: 13000000 INFO @ Sat, 15 Jan 2022 21:55:04: 14000000 INFO @ Sat, 15 Jan 2022 21:55:09: 15000000 INFO @ Sat, 15 Jan 2022 21:55:14: #1 tag size is determined as 25 bps INFO @ Sat, 15 Jan 2022 21:55:14: #1 tag size = 25 INFO @ Sat, 15 Jan 2022 21:55:14: #1 total tags in treatment: 7720187 INFO @ Sat, 15 Jan 2022 21:55:14: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:55:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:55:14: #1 tags after filtering in treatment: 5885965 INFO @ Sat, 15 Jan 2022 21:55:14: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 21:55:14: #1 finished! INFO @ Sat, 15 Jan 2022 21:55:14: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:55:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:55:15: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:55:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:55:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4623314/SRX4623314.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623314/SRX4623314.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623314/SRX4623314.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623314/SRX4623314.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling