Job ID = 14521865 SRX = SRX4623306 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 19053685 spots for SRR7767711/SRR7767711.sra Written 19053685 spots for SRR7767711/SRR7767711.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:10 19053685 reads; of these: 19053685 (100.00%) were paired; of these: 1136283 (5.96%) aligned concordantly 0 times 15426620 (80.96%) aligned concordantly exactly 1 time 2490782 (13.07%) aligned concordantly >1 times ---- 1136283 pairs aligned concordantly 0 times; of these: 547412 (48.18%) aligned discordantly 1 time ---- 588871 pairs aligned 0 times concordantly or discordantly; of these: 1177742 mates make up the pairs; of these: 699253 (59.37%) aligned 0 times 241274 (20.49%) aligned exactly 1 time 237215 (20.14%) aligned >1 times 98.17% overall alignment rate Time searching: 00:07:10 Overall time: 00:07:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 406521 / 18333627 = 0.0222 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:59:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4623306/SRX4623306.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4623306/SRX4623306.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4623306/SRX4623306.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4623306/SRX4623306.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:59:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:59:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:59:56: 1000000 INFO @ Sat, 15 Jan 2022 22:00:01: 2000000 INFO @ Sat, 15 Jan 2022 22:00:05: 3000000 INFO @ Sat, 15 Jan 2022 22:00:10: 4000000 INFO @ Sat, 15 Jan 2022 22:00:14: 5000000 INFO @ Sat, 15 Jan 2022 22:00:18: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:00:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4623306/SRX4623306.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4623306/SRX4623306.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4623306/SRX4623306.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4623306/SRX4623306.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:00:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:00:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:00:23: 7000000 INFO @ Sat, 15 Jan 2022 22:00:27: 1000000 INFO @ Sat, 15 Jan 2022 22:00:28: 8000000 INFO @ Sat, 15 Jan 2022 22:00:32: 2000000 INFO @ Sat, 15 Jan 2022 22:00:33: 9000000 INFO @ Sat, 15 Jan 2022 22:00:38: 3000000 INFO @ Sat, 15 Jan 2022 22:00:39: 10000000 INFO @ Sat, 15 Jan 2022 22:00:43: 4000000 INFO @ Sat, 15 Jan 2022 22:00:44: 11000000 INFO @ Sat, 15 Jan 2022 22:00:48: 5000000 INFO @ Sat, 15 Jan 2022 22:00:49: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:00:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4623306/SRX4623306.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4623306/SRX4623306.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4623306/SRX4623306.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4623306/SRX4623306.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:00:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:00:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:00:54: 6000000 INFO @ Sat, 15 Jan 2022 22:00:54: 13000000 INFO @ Sat, 15 Jan 2022 22:00:57: 1000000 INFO @ Sat, 15 Jan 2022 22:00:59: 7000000 INFO @ Sat, 15 Jan 2022 22:00:59: 14000000 INFO @ Sat, 15 Jan 2022 22:01:02: 2000000 INFO @ Sat, 15 Jan 2022 22:01:04: 8000000 INFO @ Sat, 15 Jan 2022 22:01:05: 15000000 INFO @ Sat, 15 Jan 2022 22:01:08: 3000000 INFO @ Sat, 15 Jan 2022 22:01:09: 9000000 INFO @ Sat, 15 Jan 2022 22:01:10: 16000000 INFO @ Sat, 15 Jan 2022 22:01:13: 4000000 INFO @ Sat, 15 Jan 2022 22:01:15: 17000000 INFO @ Sat, 15 Jan 2022 22:01:15: 10000000 INFO @ Sat, 15 Jan 2022 22:01:18: 5000000 INFO @ Sat, 15 Jan 2022 22:01:20: 18000000 INFO @ Sat, 15 Jan 2022 22:01:21: 11000000 INFO @ Sat, 15 Jan 2022 22:01:23: 6000000 INFO @ Sat, 15 Jan 2022 22:01:26: 19000000 INFO @ Sat, 15 Jan 2022 22:01:26: 12000000 INFO @ Sat, 15 Jan 2022 22:01:29: 7000000 INFO @ Sat, 15 Jan 2022 22:01:31: 20000000 INFO @ Sat, 15 Jan 2022 22:01:31: 13000000 INFO @ Sat, 15 Jan 2022 22:01:34: 8000000 INFO @ Sat, 15 Jan 2022 22:01:36: 21000000 INFO @ Sat, 15 Jan 2022 22:01:36: 14000000 INFO @ Sat, 15 Jan 2022 22:01:39: 9000000 INFO @ Sat, 15 Jan 2022 22:01:41: 22000000 INFO @ Sat, 15 Jan 2022 22:01:42: 15000000 INFO @ Sat, 15 Jan 2022 22:01:44: 10000000 INFO @ Sat, 15 Jan 2022 22:01:47: 16000000 INFO @ Sat, 15 Jan 2022 22:01:47: 23000000 INFO @ Sat, 15 Jan 2022 22:01:50: 11000000 INFO @ Sat, 15 Jan 2022 22:01:52: 17000000 INFO @ Sat, 15 Jan 2022 22:01:52: 24000000 INFO @ Sat, 15 Jan 2022 22:01:55: 12000000 INFO @ Sat, 15 Jan 2022 22:01:57: 18000000 INFO @ Sat, 15 Jan 2022 22:01:58: 25000000 INFO @ Sat, 15 Jan 2022 22:02:00: 13000000 INFO @ Sat, 15 Jan 2022 22:02:03: 19000000 INFO @ Sat, 15 Jan 2022 22:02:03: 26000000 INFO @ Sat, 15 Jan 2022 22:02:06: 14000000 INFO @ Sat, 15 Jan 2022 22:02:08: 20000000 INFO @ Sat, 15 Jan 2022 22:02:08: 27000000 INFO @ Sat, 15 Jan 2022 22:02:11: 15000000 INFO @ Sat, 15 Jan 2022 22:02:13: 21000000 INFO @ Sat, 15 Jan 2022 22:02:13: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:02:17: 16000000 INFO @ Sat, 15 Jan 2022 22:02:19: 29000000 INFO @ Sat, 15 Jan 2022 22:02:19: 22000000 INFO @ Sat, 15 Jan 2022 22:02:23: 17000000 INFO @ Sat, 15 Jan 2022 22:02:24: 30000000 INFO @ Sat, 15 Jan 2022 22:02:24: 23000000 INFO @ Sat, 15 Jan 2022 22:02:29: 18000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:02:30: 31000000 INFO @ Sat, 15 Jan 2022 22:02:30: 24000000 INFO @ Sat, 15 Jan 2022 22:02:34: 19000000 INFO @ Sat, 15 Jan 2022 22:02:35: 32000000 INFO @ Sat, 15 Jan 2022 22:02:36: 25000000 INFO @ Sat, 15 Jan 2022 22:02:40: 20000000 INFO @ Sat, 15 Jan 2022 22:02:41: 33000000 INFO @ Sat, 15 Jan 2022 22:02:41: 26000000 INFO @ Sat, 15 Jan 2022 22:02:45: 21000000 INFO @ Sat, 15 Jan 2022 22:02:47: 34000000 INFO @ Sat, 15 Jan 2022 22:02:47: 27000000 INFO @ Sat, 15 Jan 2022 22:02:51: 22000000 INFO @ Sat, 15 Jan 2022 22:02:52: 35000000 INFO @ Sat, 15 Jan 2022 22:02:52: 28000000 INFO @ Sat, 15 Jan 2022 22:02:57: 23000000 INFO @ Sat, 15 Jan 2022 22:02:58: 36000000 INFO @ Sat, 15 Jan 2022 22:02:58: 29000000 INFO @ Sat, 15 Jan 2022 22:03:01: #1 tag size is determined as 25 bps INFO @ Sat, 15 Jan 2022 22:03:01: #1 tag size = 25 INFO @ Sat, 15 Jan 2022 22:03:01: #1 total tags in treatment: 17512306 INFO @ Sat, 15 Jan 2022 22:03:01: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:03:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:03:01: #1 tags after filtering in treatment: 11170726 INFO @ Sat, 15 Jan 2022 22:03:01: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 15 Jan 2022 22:03:01: #1 finished! INFO @ Sat, 15 Jan 2022 22:03:01: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:03:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:03:02: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:03:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:03:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4623306/SRX4623306.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623306/SRX4623306.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623306/SRX4623306.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623306/SRX4623306.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:03:02: 24000000 INFO @ Sat, 15 Jan 2022 22:03:03: 30000000 INFO @ Sat, 15 Jan 2022 22:03:07: 25000000 INFO @ Sat, 15 Jan 2022 22:03:09: 31000000 INFO @ Sat, 15 Jan 2022 22:03:13: 26000000 INFO @ Sat, 15 Jan 2022 22:03:14: 32000000 INFO @ Sat, 15 Jan 2022 22:03:18: 27000000 INFO @ Sat, 15 Jan 2022 22:03:20: 33000000 INFO @ Sat, 15 Jan 2022 22:03:24: 28000000 INFO @ Sat, 15 Jan 2022 22:03:25: 34000000 INFO @ Sat, 15 Jan 2022 22:03:29: 29000000 INFO @ Sat, 15 Jan 2022 22:03:30: 35000000 INFO @ Sat, 15 Jan 2022 22:03:34: 30000000 INFO @ Sat, 15 Jan 2022 22:03:36: 36000000 INFO @ Sat, 15 Jan 2022 22:03:39: #1 tag size is determined as 25 bps INFO @ Sat, 15 Jan 2022 22:03:39: #1 tag size = 25 INFO @ Sat, 15 Jan 2022 22:03:39: #1 total tags in treatment: 17512306 INFO @ Sat, 15 Jan 2022 22:03:39: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:03:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:03:39: #1 tags after filtering in treatment: 11170726 INFO @ Sat, 15 Jan 2022 22:03:39: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 15 Jan 2022 22:03:39: #1 finished! INFO @ Sat, 15 Jan 2022 22:03:39: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:03:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:03:40: 31000000 INFO @ Sat, 15 Jan 2022 22:03:40: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:03:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:03:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4623306/SRX4623306.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623306/SRX4623306.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623306/SRX4623306.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623306/SRX4623306.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:03:45: 32000000 INFO @ Sat, 15 Jan 2022 22:03:50: 33000000 INFO @ Sat, 15 Jan 2022 22:03:54: 34000000 INFO @ Sat, 15 Jan 2022 22:03:59: 35000000 INFO @ Sat, 15 Jan 2022 22:04:04: 36000000 INFO @ Sat, 15 Jan 2022 22:04:06: #1 tag size is determined as 25 bps INFO @ Sat, 15 Jan 2022 22:04:06: #1 tag size = 25 INFO @ Sat, 15 Jan 2022 22:04:06: #1 total tags in treatment: 17512306 INFO @ Sat, 15 Jan 2022 22:04:06: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:04:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:04:07: #1 tags after filtering in treatment: 11170726 INFO @ Sat, 15 Jan 2022 22:04:07: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 15 Jan 2022 22:04:07: #1 finished! INFO @ Sat, 15 Jan 2022 22:04:07: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:04:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:04:07: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:04:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:04:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4623306/SRX4623306.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623306/SRX4623306.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623306/SRX4623306.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623306/SRX4623306.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling