Job ID = 14521864 SRX = SRX4623305 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 16032753 spots for SRR7767710/SRR7767710.sra Written 16032753 spots for SRR7767710/SRR7767710.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:08 16032753 reads; of these: 16032753 (100.00%) were paired; of these: 928237 (5.79%) aligned concordantly 0 times 13055220 (81.43%) aligned concordantly exactly 1 time 2049296 (12.78%) aligned concordantly >1 times ---- 928237 pairs aligned concordantly 0 times; of these: 454906 (49.01%) aligned discordantly 1 time ---- 473331 pairs aligned 0 times concordantly or discordantly; of these: 946662 mates make up the pairs; of these: 537238 (56.75%) aligned 0 times 214943 (22.71%) aligned exactly 1 time 194481 (20.54%) aligned >1 times 98.32% overall alignment rate Time searching: 00:09:08 Overall time: 00:09:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 401058 / 15415070 = 0.0260 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:03:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4623305/SRX4623305.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4623305/SRX4623305.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4623305/SRX4623305.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4623305/SRX4623305.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:03:25: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:03:25: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:03:32: 1000000 INFO @ Sat, 15 Jan 2022 22:03:39: 2000000 INFO @ Sat, 15 Jan 2022 22:03:46: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:03:53: 4000000 INFO @ Sat, 15 Jan 2022 22:03:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4623305/SRX4623305.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4623305/SRX4623305.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4623305/SRX4623305.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4623305/SRX4623305.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:03:55: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:03:55: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:04:01: 5000000 INFO @ Sat, 15 Jan 2022 22:04:03: 1000000 INFO @ Sat, 15 Jan 2022 22:04:08: 6000000 INFO @ Sat, 15 Jan 2022 22:04:11: 2000000 INFO @ Sat, 15 Jan 2022 22:04:16: 7000000 INFO @ Sat, 15 Jan 2022 22:04:18: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:04:23: 8000000 INFO @ Sat, 15 Jan 2022 22:04:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4623305/SRX4623305.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4623305/SRX4623305.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4623305/SRX4623305.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4623305/SRX4623305.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:04:25: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:04:25: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:04:26: 4000000 INFO @ Sat, 15 Jan 2022 22:04:31: 9000000 INFO @ Sat, 15 Jan 2022 22:04:34: 5000000 INFO @ Sat, 15 Jan 2022 22:04:35: 1000000 INFO @ Sat, 15 Jan 2022 22:04:38: 10000000 INFO @ Sat, 15 Jan 2022 22:04:41: 6000000 INFO @ Sat, 15 Jan 2022 22:04:45: 2000000 INFO @ Sat, 15 Jan 2022 22:04:46: 11000000 INFO @ Sat, 15 Jan 2022 22:04:49: 7000000 INFO @ Sat, 15 Jan 2022 22:04:54: 12000000 INFO @ Sat, 15 Jan 2022 22:04:54: 3000000 INFO @ Sat, 15 Jan 2022 22:04:57: 8000000 INFO @ Sat, 15 Jan 2022 22:05:02: 13000000 INFO @ Sat, 15 Jan 2022 22:05:04: 4000000 INFO @ Sat, 15 Jan 2022 22:05:05: 9000000 INFO @ Sat, 15 Jan 2022 22:05:09: 14000000 INFO @ Sat, 15 Jan 2022 22:05:12: 10000000 INFO @ Sat, 15 Jan 2022 22:05:13: 5000000 INFO @ Sat, 15 Jan 2022 22:05:17: 15000000 INFO @ Sat, 15 Jan 2022 22:05:19: 11000000 INFO @ Sat, 15 Jan 2022 22:05:23: 6000000 INFO @ Sat, 15 Jan 2022 22:05:25: 16000000 INFO @ Sat, 15 Jan 2022 22:05:27: 12000000 INFO @ Sat, 15 Jan 2022 22:05:33: 17000000 INFO @ Sat, 15 Jan 2022 22:05:33: 7000000 INFO @ Sat, 15 Jan 2022 22:05:35: 13000000 INFO @ Sat, 15 Jan 2022 22:05:40: 18000000 INFO @ Sat, 15 Jan 2022 22:05:43: 14000000 INFO @ Sat, 15 Jan 2022 22:05:43: 8000000 INFO @ Sat, 15 Jan 2022 22:05:48: 19000000 INFO @ Sat, 15 Jan 2022 22:05:50: 15000000 INFO @ Sat, 15 Jan 2022 22:05:53: 9000000 INFO @ Sat, 15 Jan 2022 22:05:56: 20000000 INFO @ Sat, 15 Jan 2022 22:05:59: 16000000 INFO @ Sat, 15 Jan 2022 22:06:03: 10000000 INFO @ Sat, 15 Jan 2022 22:06:04: 21000000 INFO @ Sat, 15 Jan 2022 22:06:06: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:06:12: 22000000 INFO @ Sat, 15 Jan 2022 22:06:13: 11000000 INFO @ Sat, 15 Jan 2022 22:06:14: 18000000 INFO @ Sat, 15 Jan 2022 22:06:20: 23000000 INFO @ Sat, 15 Jan 2022 22:06:22: 19000000 INFO @ Sat, 15 Jan 2022 22:06:23: 12000000 INFO @ Sat, 15 Jan 2022 22:06:28: 24000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:06:30: 20000000 INFO @ Sat, 15 Jan 2022 22:06:33: 13000000 INFO @ Sat, 15 Jan 2022 22:06:36: 25000000 INFO @ Sat, 15 Jan 2022 22:06:38: 21000000 INFO @ Sat, 15 Jan 2022 22:06:43: 14000000 INFO @ Sat, 15 Jan 2022 22:06:44: 26000000 INFO @ Sat, 15 Jan 2022 22:06:45: 22000000 INFO @ Sat, 15 Jan 2022 22:06:51: 27000000 INFO @ Sat, 15 Jan 2022 22:06:52: 15000000 INFO @ Sat, 15 Jan 2022 22:06:52: 23000000 INFO @ Sat, 15 Jan 2022 22:06:59: 28000000 INFO @ Sat, 15 Jan 2022 22:07:00: 24000000 INFO @ Sat, 15 Jan 2022 22:07:01: 16000000 INFO @ Sat, 15 Jan 2022 22:07:07: 29000000 INFO @ Sat, 15 Jan 2022 22:07:07: 25000000 INFO @ Sat, 15 Jan 2022 22:07:10: 17000000 INFO @ Sat, 15 Jan 2022 22:07:15: 30000000 INFO @ Sat, 15 Jan 2022 22:07:15: 26000000 INFO @ Sat, 15 Jan 2022 22:07:19: 18000000 INFO @ Sat, 15 Jan 2022 22:07:20: #1 tag size is determined as 25 bps INFO @ Sat, 15 Jan 2022 22:07:20: #1 tag size = 25 INFO @ Sat, 15 Jan 2022 22:07:20: #1 total tags in treatment: 14704730 INFO @ Sat, 15 Jan 2022 22:07:20: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:07:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:07:21: #1 tags after filtering in treatment: 9539226 INFO @ Sat, 15 Jan 2022 22:07:21: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 15 Jan 2022 22:07:21: #1 finished! INFO @ Sat, 15 Jan 2022 22:07:21: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:07:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:07:21: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:07:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:07:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4623305/SRX4623305.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623305/SRX4623305.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623305/SRX4623305.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623305/SRX4623305.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:07:22: 27000000 INFO @ Sat, 15 Jan 2022 22:07:27: 19000000 INFO @ Sat, 15 Jan 2022 22:07:30: 28000000 INFO @ Sat, 15 Jan 2022 22:07:36: 20000000 INFO @ Sat, 15 Jan 2022 22:07:37: 29000000 INFO @ Sat, 15 Jan 2022 22:07:44: 30000000 INFO @ Sat, 15 Jan 2022 22:07:46: 21000000 INFO @ Sat, 15 Jan 2022 22:07:50: #1 tag size is determined as 25 bps INFO @ Sat, 15 Jan 2022 22:07:50: #1 tag size = 25 INFO @ Sat, 15 Jan 2022 22:07:50: #1 total tags in treatment: 14704730 INFO @ Sat, 15 Jan 2022 22:07:50: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:07:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:07:50: #1 tags after filtering in treatment: 9539226 INFO @ Sat, 15 Jan 2022 22:07:50: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 15 Jan 2022 22:07:50: #1 finished! INFO @ Sat, 15 Jan 2022 22:07:50: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:07:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:07:51: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:07:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:07:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4623305/SRX4623305.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623305/SRX4623305.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623305/SRX4623305.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623305/SRX4623305.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:07:55: 22000000 INFO @ Sat, 15 Jan 2022 22:08:05: 23000000 INFO @ Sat, 15 Jan 2022 22:08:15: 24000000 INFO @ Sat, 15 Jan 2022 22:08:26: 25000000 INFO @ Sat, 15 Jan 2022 22:08:37: 26000000 INFO @ Sat, 15 Jan 2022 22:08:47: 27000000 INFO @ Sat, 15 Jan 2022 22:08:57: 28000000 INFO @ Sat, 15 Jan 2022 22:09:06: 29000000 INFO @ Sat, 15 Jan 2022 22:09:15: 30000000 INFO @ Sat, 15 Jan 2022 22:09:22: #1 tag size is determined as 25 bps INFO @ Sat, 15 Jan 2022 22:09:22: #1 tag size = 25 INFO @ Sat, 15 Jan 2022 22:09:22: #1 total tags in treatment: 14704730 INFO @ Sat, 15 Jan 2022 22:09:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:09:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:09:23: #1 tags after filtering in treatment: 9539226 INFO @ Sat, 15 Jan 2022 22:09:23: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 15 Jan 2022 22:09:23: #1 finished! INFO @ Sat, 15 Jan 2022 22:09:23: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:09:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:09:23: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:09:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:09:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4623305/SRX4623305.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623305/SRX4623305.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623305/SRX4623305.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623305/SRX4623305.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling