Job ID = 14521861 SRX = SRX4623302 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 21929877 spots for SRR7767707/SRR7767707.sra Written 21929877 spots for SRR7767707/SRR7767707.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:49 21929877 reads; of these: 21929877 (100.00%) were paired; of these: 1412821 (6.44%) aligned concordantly 0 times 17795840 (81.15%) aligned concordantly exactly 1 time 2721216 (12.41%) aligned concordantly >1 times ---- 1412821 pairs aligned concordantly 0 times; of these: 546767 (38.70%) aligned discordantly 1 time ---- 866054 pairs aligned 0 times concordantly or discordantly; of these: 1732108 mates make up the pairs; of these: 1239671 (71.57%) aligned 0 times 259491 (14.98%) aligned exactly 1 time 232946 (13.45%) aligned >1 times 97.17% overall alignment rate Time searching: 00:13:49 Overall time: 00:13:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 463081 / 20920481 = 0.0221 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:11:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4623302/SRX4623302.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4623302/SRX4623302.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4623302/SRX4623302.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4623302/SRX4623302.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:11:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:11:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:11:46: 1000000 INFO @ Sat, 15 Jan 2022 22:11:55: 2000000 INFO @ Sat, 15 Jan 2022 22:12:04: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:12:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4623302/SRX4623302.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4623302/SRX4623302.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4623302/SRX4623302.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4623302/SRX4623302.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:12:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:12:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:12:13: 4000000 INFO @ Sat, 15 Jan 2022 22:12:17: 1000000 INFO @ Sat, 15 Jan 2022 22:12:22: 5000000 INFO @ Sat, 15 Jan 2022 22:12:25: 2000000 INFO @ Sat, 15 Jan 2022 22:12:32: 6000000 INFO @ Sat, 15 Jan 2022 22:12:34: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:12:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4623302/SRX4623302.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4623302/SRX4623302.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4623302/SRX4623302.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4623302/SRX4623302.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:12:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:12:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:12:41: 7000000 INFO @ Sat, 15 Jan 2022 22:12:43: 4000000 INFO @ Sat, 15 Jan 2022 22:12:49: 1000000 INFO @ Sat, 15 Jan 2022 22:12:50: 8000000 INFO @ Sat, 15 Jan 2022 22:12:52: 5000000 INFO @ Sat, 15 Jan 2022 22:12:59: 2000000 INFO @ Sat, 15 Jan 2022 22:12:59: 9000000 INFO @ Sat, 15 Jan 2022 22:13:01: 6000000 INFO @ Sat, 15 Jan 2022 22:13:08: 10000000 INFO @ Sat, 15 Jan 2022 22:13:09: 3000000 INFO @ Sat, 15 Jan 2022 22:13:10: 7000000 INFO @ Sat, 15 Jan 2022 22:13:17: 11000000 INFO @ Sat, 15 Jan 2022 22:13:19: 8000000 INFO @ Sat, 15 Jan 2022 22:13:19: 4000000 INFO @ Sat, 15 Jan 2022 22:13:25: 12000000 INFO @ Sat, 15 Jan 2022 22:13:27: 9000000 INFO @ Sat, 15 Jan 2022 22:13:30: 5000000 INFO @ Sat, 15 Jan 2022 22:13:34: 13000000 INFO @ Sat, 15 Jan 2022 22:13:36: 10000000 INFO @ Sat, 15 Jan 2022 22:13:40: 6000000 INFO @ Sat, 15 Jan 2022 22:13:43: 14000000 INFO @ Sat, 15 Jan 2022 22:13:44: 11000000 INFO @ Sat, 15 Jan 2022 22:13:50: 7000000 INFO @ Sat, 15 Jan 2022 22:13:52: 15000000 INFO @ Sat, 15 Jan 2022 22:13:52: 12000000 INFO @ Sat, 15 Jan 2022 22:14:01: 8000000 INFO @ Sat, 15 Jan 2022 22:14:01: 13000000 INFO @ Sat, 15 Jan 2022 22:14:02: 16000000 INFO @ Sat, 15 Jan 2022 22:14:10: 14000000 INFO @ Sat, 15 Jan 2022 22:14:10: 17000000 INFO @ Sat, 15 Jan 2022 22:14:11: 9000000 INFO @ Sat, 15 Jan 2022 22:14:18: 15000000 INFO @ Sat, 15 Jan 2022 22:14:18: 18000000 INFO @ Sat, 15 Jan 2022 22:14:21: 10000000 INFO @ Sat, 15 Jan 2022 22:14:27: 16000000 INFO @ Sat, 15 Jan 2022 22:14:27: 19000000 INFO @ Sat, 15 Jan 2022 22:14:31: 11000000 INFO @ Sat, 15 Jan 2022 22:14:35: 17000000 INFO @ Sat, 15 Jan 2022 22:14:36: 20000000 INFO @ Sat, 15 Jan 2022 22:14:41: 12000000 INFO @ Sat, 15 Jan 2022 22:14:44: 18000000 INFO @ Sat, 15 Jan 2022 22:14:44: 21000000 INFO @ Sat, 15 Jan 2022 22:14:51: 13000000 INFO @ Sat, 15 Jan 2022 22:14:53: 22000000 INFO @ Sat, 15 Jan 2022 22:14:53: 19000000 INFO @ Sat, 15 Jan 2022 22:15:01: 14000000 INFO @ Sat, 15 Jan 2022 22:15:01: 23000000 INFO @ Sat, 15 Jan 2022 22:15:01: 20000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:15:10: 24000000 INFO @ Sat, 15 Jan 2022 22:15:10: 21000000 INFO @ Sat, 15 Jan 2022 22:15:11: 15000000 INFO @ Sat, 15 Jan 2022 22:15:19: 25000000 INFO @ Sat, 15 Jan 2022 22:15:19: 22000000 INFO @ Sat, 15 Jan 2022 22:15:21: 16000000 INFO @ Sat, 15 Jan 2022 22:15:28: 26000000 INFO @ Sat, 15 Jan 2022 22:15:28: 23000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:15:31: 17000000 INFO @ Sat, 15 Jan 2022 22:15:37: 27000000 INFO @ Sat, 15 Jan 2022 22:15:37: 24000000 INFO @ Sat, 15 Jan 2022 22:15:41: 18000000 INFO @ Sat, 15 Jan 2022 22:15:46: 28000000 INFO @ Sat, 15 Jan 2022 22:15:46: 25000000 INFO @ Sat, 15 Jan 2022 22:15:51: 19000000 INFO @ Sat, 15 Jan 2022 22:15:55: 29000000 INFO @ Sat, 15 Jan 2022 22:15:55: 26000000 INFO @ Sat, 15 Jan 2022 22:16:01: 20000000 INFO @ Sat, 15 Jan 2022 22:16:04: 30000000 INFO @ Sat, 15 Jan 2022 22:16:04: 27000000 INFO @ Sat, 15 Jan 2022 22:16:11: 21000000 INFO @ Sat, 15 Jan 2022 22:16:13: 31000000 INFO @ Sat, 15 Jan 2022 22:16:13: 28000000 INFO @ Sat, 15 Jan 2022 22:16:21: 22000000 INFO @ Sat, 15 Jan 2022 22:16:22: 32000000 INFO @ Sat, 15 Jan 2022 22:16:22: 29000000 INFO @ Sat, 15 Jan 2022 22:16:30: 33000000 INFO @ Sat, 15 Jan 2022 22:16:32: 23000000 INFO @ Sat, 15 Jan 2022 22:16:32: 30000000 INFO @ Sat, 15 Jan 2022 22:16:39: 34000000 INFO @ Sat, 15 Jan 2022 22:16:41: 31000000 INFO @ Sat, 15 Jan 2022 22:16:42: 24000000 INFO @ Sat, 15 Jan 2022 22:16:47: 35000000 INFO @ Sat, 15 Jan 2022 22:16:50: 32000000 INFO @ Sat, 15 Jan 2022 22:16:52: 25000000 INFO @ Sat, 15 Jan 2022 22:16:56: 36000000 INFO @ Sat, 15 Jan 2022 22:16:59: 33000000 INFO @ Sat, 15 Jan 2022 22:17:02: 26000000 INFO @ Sat, 15 Jan 2022 22:17:04: 37000000 INFO @ Sat, 15 Jan 2022 22:17:08: 34000000 INFO @ Sat, 15 Jan 2022 22:17:12: 27000000 INFO @ Sat, 15 Jan 2022 22:17:13: 38000000 INFO @ Sat, 15 Jan 2022 22:17:17: 35000000 INFO @ Sat, 15 Jan 2022 22:17:21: 28000000 INFO @ Sat, 15 Jan 2022 22:17:22: 39000000 INFO @ Sat, 15 Jan 2022 22:17:26: 36000000 INFO @ Sat, 15 Jan 2022 22:17:30: 40000000 INFO @ Sat, 15 Jan 2022 22:17:31: 29000000 INFO @ Sat, 15 Jan 2022 22:17:34: 37000000 INFO @ Sat, 15 Jan 2022 22:17:39: 41000000 INFO @ Sat, 15 Jan 2022 22:17:40: 30000000 INFO @ Sat, 15 Jan 2022 22:17:43: 38000000 INFO @ Sat, 15 Jan 2022 22:17:44: #1 tag size is determined as 25 bps INFO @ Sat, 15 Jan 2022 22:17:44: #1 tag size = 25 INFO @ Sat, 15 Jan 2022 22:17:44: #1 total tags in treatment: 20055455 INFO @ Sat, 15 Jan 2022 22:17:44: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:17:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:17:45: #1 tags after filtering in treatment: 12455570 INFO @ Sat, 15 Jan 2022 22:17:45: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 15 Jan 2022 22:17:45: #1 finished! INFO @ Sat, 15 Jan 2022 22:17:45: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:17:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:17:46: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:17:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:17:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4623302/SRX4623302.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623302/SRX4623302.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623302/SRX4623302.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623302/SRX4623302.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:17:50: 31000000 INFO @ Sat, 15 Jan 2022 22:17:51: 39000000 INFO @ Sat, 15 Jan 2022 22:18:00: 40000000 INFO @ Sat, 15 Jan 2022 22:18:00: 32000000 INFO @ Sat, 15 Jan 2022 22:18:08: 41000000 INFO @ Sat, 15 Jan 2022 22:18:10: 33000000 INFO @ Sat, 15 Jan 2022 22:18:15: #1 tag size is determined as 25 bps INFO @ Sat, 15 Jan 2022 22:18:15: #1 tag size = 25 INFO @ Sat, 15 Jan 2022 22:18:15: #1 total tags in treatment: 20055455 INFO @ Sat, 15 Jan 2022 22:18:15: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:18:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:18:15: #1 tags after filtering in treatment: 12455570 INFO @ Sat, 15 Jan 2022 22:18:15: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 15 Jan 2022 22:18:15: #1 finished! INFO @ Sat, 15 Jan 2022 22:18:15: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:18:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:18:16: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:18:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:18:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4623302/SRX4623302.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623302/SRX4623302.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623302/SRX4623302.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623302/SRX4623302.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:18:20: 34000000 INFO @ Sat, 15 Jan 2022 22:18:30: 35000000 INFO @ Sat, 15 Jan 2022 22:18:40: 36000000 INFO @ Sat, 15 Jan 2022 22:18:50: 37000000 INFO @ Sat, 15 Jan 2022 22:18:59: 38000000 INFO @ Sat, 15 Jan 2022 22:19:09: 39000000 INFO @ Sat, 15 Jan 2022 22:19:19: 40000000 INFO @ Sat, 15 Jan 2022 22:19:30: 41000000 INFO @ Sat, 15 Jan 2022 22:19:38: #1 tag size is determined as 25 bps INFO @ Sat, 15 Jan 2022 22:19:38: #1 tag size = 25 INFO @ Sat, 15 Jan 2022 22:19:38: #1 total tags in treatment: 20055455 INFO @ Sat, 15 Jan 2022 22:19:38: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:19:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:19:38: #1 tags after filtering in treatment: 12455570 INFO @ Sat, 15 Jan 2022 22:19:38: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 15 Jan 2022 22:19:38: #1 finished! INFO @ Sat, 15 Jan 2022 22:19:38: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:19:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:19:39: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:19:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:19:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4623302/SRX4623302.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623302/SRX4623302.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623302/SRX4623302.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623302/SRX4623302.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling